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Effect of Netrin-1 Anti-Inflammatory Factor on Acute Lung Injury in Sepsis Rats

BACKGROUNDS: Acute lung injury (ALI) often occurs early and seriously in the progress of sepsis. Netrin-1 is demonstrated to be an effective anti-inflammatory agent. However, whether netrin-1 can relieve sepsis-induced ALI remains unknown. MATERIAL/METHODS: The sepsis rat model was built with the me...

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Detalles Bibliográficos
Autores principales: Liu, Jisong, Du, Juan, Cheng, Xiu, Zhang, Xiangzhou, Li, Yong, Fu, Xiujun, Chen, Xulin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820332/
https://www.ncbi.nlm.nih.gov/pubmed/31639817
http://dx.doi.org/10.12659/MSM.917279
Descripción
Sumario:BACKGROUNDS: Acute lung injury (ALI) often occurs early and seriously in the progress of sepsis. Netrin-1 is demonstrated to be an effective anti-inflammatory agent. However, whether netrin-1 can relieve sepsis-induced ALI remains unknown. MATERIAL/METHODS: The sepsis rat model was built with the method of cecal ligation and puncture (CLP). The lung tissue changes were represented as the results of hematoxylin-eosin (HE) staining, wet-to-dry (W/D) ratio, Western blot analysis, and immunohistochemistry. An in vitro lung injury model was simulated with LPS-induced BEAS-2B cells. The cell transfection effects were evaluated by Western blot analysis and RT-qPCR analysis. TNF-α, IL-1β, and IL-6 levels were detected by Western blot analysis in LPS-induced BEAS-2B cells. RESULTS: Obvious inflammation caused by sepsis appeared in lung tissues with the increase of the W/D ratio and expression of inflammatory cytokines. Netrin-1 and its receptor UNC5B were reduced in sepsis. However, upregulation of netrin-1 alleviated the levels of inflammation and increased the UNC5B levels in BEAS-2B cells. CONCLUSIONS: Netrin-1 protects against ALI in sepsis rats through its anti-inflammation effect and may provide a novel treatment to prevent lung injury caused by sepsis.