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Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study

METHOD: A cohort study was carried out for a year to evaluate the presence of G-to-A transition in 5′-untranslated region of ankylosis human (ANKH) gene in Indian Khatri patients (closely resembling Europeans of primary knee osteoarthritis (OA), residing in Lucknow, India. RESULTS: In the total part...

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Autores principales: Kumar, Puneet, Sharma, Aarti, Das, Siddharth, Srivastava, Ragini, Gupta, Nikhil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820404/
https://www.ncbi.nlm.nih.gov/pubmed/31681671
http://dx.doi.org/10.4103/jfmpc.jfmpc_471_19
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author Kumar, Puneet
Sharma, Aarti
Das, Siddharth
Srivastava, Ragini
Gupta, Nikhil
author_facet Kumar, Puneet
Sharma, Aarti
Das, Siddharth
Srivastava, Ragini
Gupta, Nikhil
author_sort Kumar, Puneet
collection PubMed
description METHOD: A cohort study was carried out for a year to evaluate the presence of G-to-A transition in 5′-untranslated region of ankylosis human (ANKH) gene in Indian Khatri patients (closely resembling Europeans of primary knee osteoarthritis (OA), residing in Lucknow, India. RESULTS: In the total participants, 25 were Khatri primary knee OA patients (cases) residing in Lucknow and 101 were random blood donors’ samples (controls) collected from a blood bank. All were studied for the abovementioned mutation using real-time polymerase chain reaction (RT-PCR). GG genotype was present in 72.3% of controls and 76% of Khatri knee OA patients. The studied G-to-A mutation was found to be positive in 24.8% of controls and 16% of cases, odds ratio (95% confidence interval) being 0.6 (0.19–1.98, P = 0.42). The frequency of AA (D) genotype found around 3% (cases) and 8% (controls) with P value of 0.70. The combined frequency of both homozygous and heterozygous mutation (GA and AA) in the studied population was 28 (27.7%) in controls and 6 (24%) in cases with the odds ratio (OD) ratio of 0.82 (0.29–2.27, P = 0.70). No significant differences were observed at both genotype and allelic level in the distribution of ANKH-4 G-to-A gene polymorphism in studied subjects. CONCLUSION: This study did not show any significant G to A mutation in the studied subjects.
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spelling pubmed-68204042019-11-01 Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study Kumar, Puneet Sharma, Aarti Das, Siddharth Srivastava, Ragini Gupta, Nikhil J Family Med Prim Care Original Article METHOD: A cohort study was carried out for a year to evaluate the presence of G-to-A transition in 5′-untranslated region of ankylosis human (ANKH) gene in Indian Khatri patients (closely resembling Europeans of primary knee osteoarthritis (OA), residing in Lucknow, India. RESULTS: In the total participants, 25 were Khatri primary knee OA patients (cases) residing in Lucknow and 101 were random blood donors’ samples (controls) collected from a blood bank. All were studied for the abovementioned mutation using real-time polymerase chain reaction (RT-PCR). GG genotype was present in 72.3% of controls and 76% of Khatri knee OA patients. The studied G-to-A mutation was found to be positive in 24.8% of controls and 16% of cases, odds ratio (95% confidence interval) being 0.6 (0.19–1.98, P = 0.42). The frequency of AA (D) genotype found around 3% (cases) and 8% (controls) with P value of 0.70. The combined frequency of both homozygous and heterozygous mutation (GA and AA) in the studied population was 28 (27.7%) in controls and 6 (24%) in cases with the odds ratio (OD) ratio of 0.82 (0.29–2.27, P = 0.70). No significant differences were observed at both genotype and allelic level in the distribution of ANKH-4 G-to-A gene polymorphism in studied subjects. CONCLUSION: This study did not show any significant G to A mutation in the studied subjects. Wolters Kluwer - Medknow 2019-09-30 /pmc/articles/PMC6820404/ /pubmed/31681671 http://dx.doi.org/10.4103/jfmpc.jfmpc_471_19 Text en Copyright: © 2019 Journal of Family Medicine and Primary Care http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Kumar, Puneet
Sharma, Aarti
Das, Siddharth
Srivastava, Ragini
Gupta, Nikhil
Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study
title Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study
title_full Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study
title_fullStr Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study
title_full_unstemmed Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study
title_short Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study
title_sort association of 4-basepair g-to-a transition in the 5′-untranslated region of ankh gene with selected patients of primary knee osteoarthritis: a cross sectional study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820404/
https://www.ncbi.nlm.nih.gov/pubmed/31681671
http://dx.doi.org/10.4103/jfmpc.jfmpc_471_19
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