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Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana

Plant root system architecture in response to nitrate availability represents a notable example to study developmental plasticity, but the underlying mechanism remains largely unknown. Xyloglucan endotransglucosylases (XTHs) play a critical role in cell wall biosynthesis. Here we assessed the gene e...

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Autores principales: Xu, Peipei, Cai, Weiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821136/
https://www.ncbi.nlm.nih.gov/pubmed/31626627
http://dx.doi.org/10.1371/journal.pgen.1008465
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author Xu, Peipei
Cai, Weiming
author_facet Xu, Peipei
Cai, Weiming
author_sort Xu, Peipei
collection PubMed
description Plant root system architecture in response to nitrate availability represents a notable example to study developmental plasticity, but the underlying mechanism remains largely unknown. Xyloglucan endotransglucosylases (XTHs) play a critical role in cell wall biosynthesis. Here we assessed the gene expression of XTH1-11 belonging to group I of XTHs in lateral root (LR) primordia and found that XTH9 was highly expressed. Correspondingly, an xth9 mutant displayed less LR, while overexpressing XTH9 presented more LR, suggesting the potential function of XTH9 in controlling LR development. XTH9 gene mutation obviously alters the properties of the cell wall. Furthermore, nitrogen signals stimulated the expression of XTH9 to promote LRs. Genetic analysis revealed that the function of XTH9 was dependent on auxin-mediated ARF7/19 and downstream AFB3 in response to nitrogen signals. In addition, we identified another transcription factor, OBP4, that was also induced by nitrogen treatment, but the induction was much slower than that of XTH9. In contrast to XTH9, overexpressing OBP4 caused fewer LRs while OBP4 knockdown with OBP4-RNAi or an artificial miRNA silenced amiOBP4 line produced more LR. We further found OBP4 bound to the promoter of XTH9 to suppress XTH9 expression. In agreement with this, both OBP4-RNAi and crossed OBP4-RNAi & 35S::XTH9 lines led to more LR, but OBP4-RNAi & xth9 produced less LR, similar to xth9. Based on these findings we propose a novel mechanism by which OBP4 antagonistically controls XTH9 expression and the OBP4-XTH9 module elaborately sustains LR development in response to nitrate treatment.
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spelling pubmed-68211362019-11-08 Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana Xu, Peipei Cai, Weiming PLoS Genet Research Article Plant root system architecture in response to nitrate availability represents a notable example to study developmental plasticity, but the underlying mechanism remains largely unknown. Xyloglucan endotransglucosylases (XTHs) play a critical role in cell wall biosynthesis. Here we assessed the gene expression of XTH1-11 belonging to group I of XTHs in lateral root (LR) primordia and found that XTH9 was highly expressed. Correspondingly, an xth9 mutant displayed less LR, while overexpressing XTH9 presented more LR, suggesting the potential function of XTH9 in controlling LR development. XTH9 gene mutation obviously alters the properties of the cell wall. Furthermore, nitrogen signals stimulated the expression of XTH9 to promote LRs. Genetic analysis revealed that the function of XTH9 was dependent on auxin-mediated ARF7/19 and downstream AFB3 in response to nitrogen signals. In addition, we identified another transcription factor, OBP4, that was also induced by nitrogen treatment, but the induction was much slower than that of XTH9. In contrast to XTH9, overexpressing OBP4 caused fewer LRs while OBP4 knockdown with OBP4-RNAi or an artificial miRNA silenced amiOBP4 line produced more LR. We further found OBP4 bound to the promoter of XTH9 to suppress XTH9 expression. In agreement with this, both OBP4-RNAi and crossed OBP4-RNAi & 35S::XTH9 lines led to more LR, but OBP4-RNAi & xth9 produced less LR, similar to xth9. Based on these findings we propose a novel mechanism by which OBP4 antagonistically controls XTH9 expression and the OBP4-XTH9 module elaborately sustains LR development in response to nitrate treatment. Public Library of Science 2019-10-18 /pmc/articles/PMC6821136/ /pubmed/31626627 http://dx.doi.org/10.1371/journal.pgen.1008465 Text en © 2019 Xu, Cai http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xu, Peipei
Cai, Weiming
Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana
title Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana
title_full Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana
title_fullStr Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana
title_full_unstemmed Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana
title_short Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana
title_sort nitrate-responsive obp4-xth9 regulatory module controls lateral root development in arabidopsis thaliana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821136/
https://www.ncbi.nlm.nih.gov/pubmed/31626627
http://dx.doi.org/10.1371/journal.pgen.1008465
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