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Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana
Plant root system architecture in response to nitrate availability represents a notable example to study developmental plasticity, but the underlying mechanism remains largely unknown. Xyloglucan endotransglucosylases (XTHs) play a critical role in cell wall biosynthesis. Here we assessed the gene e...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821136/ https://www.ncbi.nlm.nih.gov/pubmed/31626627 http://dx.doi.org/10.1371/journal.pgen.1008465 |
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author | Xu, Peipei Cai, Weiming |
author_facet | Xu, Peipei Cai, Weiming |
author_sort | Xu, Peipei |
collection | PubMed |
description | Plant root system architecture in response to nitrate availability represents a notable example to study developmental plasticity, but the underlying mechanism remains largely unknown. Xyloglucan endotransglucosylases (XTHs) play a critical role in cell wall biosynthesis. Here we assessed the gene expression of XTH1-11 belonging to group I of XTHs in lateral root (LR) primordia and found that XTH9 was highly expressed. Correspondingly, an xth9 mutant displayed less LR, while overexpressing XTH9 presented more LR, suggesting the potential function of XTH9 in controlling LR development. XTH9 gene mutation obviously alters the properties of the cell wall. Furthermore, nitrogen signals stimulated the expression of XTH9 to promote LRs. Genetic analysis revealed that the function of XTH9 was dependent on auxin-mediated ARF7/19 and downstream AFB3 in response to nitrogen signals. In addition, we identified another transcription factor, OBP4, that was also induced by nitrogen treatment, but the induction was much slower than that of XTH9. In contrast to XTH9, overexpressing OBP4 caused fewer LRs while OBP4 knockdown with OBP4-RNAi or an artificial miRNA silenced amiOBP4 line produced more LR. We further found OBP4 bound to the promoter of XTH9 to suppress XTH9 expression. In agreement with this, both OBP4-RNAi and crossed OBP4-RNAi & 35S::XTH9 lines led to more LR, but OBP4-RNAi & xth9 produced less LR, similar to xth9. Based on these findings we propose a novel mechanism by which OBP4 antagonistically controls XTH9 expression and the OBP4-XTH9 module elaborately sustains LR development in response to nitrate treatment. |
format | Online Article Text |
id | pubmed-6821136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-68211362019-11-08 Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana Xu, Peipei Cai, Weiming PLoS Genet Research Article Plant root system architecture in response to nitrate availability represents a notable example to study developmental plasticity, but the underlying mechanism remains largely unknown. Xyloglucan endotransglucosylases (XTHs) play a critical role in cell wall biosynthesis. Here we assessed the gene expression of XTH1-11 belonging to group I of XTHs in lateral root (LR) primordia and found that XTH9 was highly expressed. Correspondingly, an xth9 mutant displayed less LR, while overexpressing XTH9 presented more LR, suggesting the potential function of XTH9 in controlling LR development. XTH9 gene mutation obviously alters the properties of the cell wall. Furthermore, nitrogen signals stimulated the expression of XTH9 to promote LRs. Genetic analysis revealed that the function of XTH9 was dependent on auxin-mediated ARF7/19 and downstream AFB3 in response to nitrogen signals. In addition, we identified another transcription factor, OBP4, that was also induced by nitrogen treatment, but the induction was much slower than that of XTH9. In contrast to XTH9, overexpressing OBP4 caused fewer LRs while OBP4 knockdown with OBP4-RNAi or an artificial miRNA silenced amiOBP4 line produced more LR. We further found OBP4 bound to the promoter of XTH9 to suppress XTH9 expression. In agreement with this, both OBP4-RNAi and crossed OBP4-RNAi & 35S::XTH9 lines led to more LR, but OBP4-RNAi & xth9 produced less LR, similar to xth9. Based on these findings we propose a novel mechanism by which OBP4 antagonistically controls XTH9 expression and the OBP4-XTH9 module elaborately sustains LR development in response to nitrate treatment. Public Library of Science 2019-10-18 /pmc/articles/PMC6821136/ /pubmed/31626627 http://dx.doi.org/10.1371/journal.pgen.1008465 Text en © 2019 Xu, Cai http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Xu, Peipei Cai, Weiming Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana |
title | Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana |
title_full | Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana |
title_fullStr | Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana |
title_full_unstemmed | Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana |
title_short | Nitrate-responsive OBP4-XTH9 regulatory module controls lateral root development in Arabidopsis thaliana |
title_sort | nitrate-responsive obp4-xth9 regulatory module controls lateral root development in arabidopsis thaliana |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821136/ https://www.ncbi.nlm.nih.gov/pubmed/31626627 http://dx.doi.org/10.1371/journal.pgen.1008465 |
work_keys_str_mv | AT xupeipei nitrateresponsiveobp4xth9regulatorymodulecontrolslateralrootdevelopmentinarabidopsisthaliana AT caiweiming nitrateresponsiveobp4xth9regulatorymodulecontrolslateralrootdevelopmentinarabidopsisthaliana |