Cargando…
Efficient inhibition of RNA self-primed extension by addition of competing 3′-capture DNA-improved RNA synthesis by T7 RNA polymerase
In vitro synthesized RNA is used widely in studies of RNA biology, biotechnology and RNA therapeutics. However, in vitro synthesized RNA often contains impurities, such as RNAs with lengths shorter and longer than the expected runoff RNA. We have recently confirmed that longer RNA products are forme...
Autores principales: | Gholamalipour, Yasaman, Johnson, William C, Martin, Craig T |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821179/ https://www.ncbi.nlm.nih.gov/pubmed/31392994 http://dx.doi.org/10.1093/nar/gkz700 |
Ejemplares similares
-
3′ end additions by T7 RNA polymerase are RNA self-templated, distributive and diverse in character—RNA-Seq analyses
por: Gholamalipour, Yasaman, et al.
Publicado: (2018) -
Synthesis of 2′-Fluoro RNA by Syn5 RNA polymerase
por: Zhu, Bin, et al.
Publicado: (2015) -
Tn5Prime, a Tn5 based 5′ capture method for single cell RNA-seq
por: Cole, Charles, et al.
Publicado: (2018) -
An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi
por: Zhou, Hongxia, et al.
Publicado: (2005) -
RNA aptamer capture of macromolecular complexes for mass spectrometry analysis
por: Ray, Judhajeet, et al.
Publicado: (2020)