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Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone

Monocarboxylate transporters (MCTs) provide transmembrane transport of monocarboxylates such as lactate and pyruvate. The present results showed that α-cyano-4-hydroxycinnamic acid (CHC), an inhibitor of MCTs, promoted osteoclast differentiation from macrophages at lower concentrations (0.1–0.3 mM)...

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Autores principales: Imai, Hiroko, Yoshimura, Kentaro, Miyamoto, Yoichi, Sasa, Kiyohito, Sugano, Marika, Chatani, Masahiro, Takami, Masamichi, Yamamoto, Matsuo, Kamijo, Ryutaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821745/
https://www.ncbi.nlm.nih.gov/pubmed/31666601
http://dx.doi.org/10.1038/s41598-019-52128-2
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author Imai, Hiroko
Yoshimura, Kentaro
Miyamoto, Yoichi
Sasa, Kiyohito
Sugano, Marika
Chatani, Masahiro
Takami, Masamichi
Yamamoto, Matsuo
Kamijo, Ryutaro
author_facet Imai, Hiroko
Yoshimura, Kentaro
Miyamoto, Yoichi
Sasa, Kiyohito
Sugano, Marika
Chatani, Masahiro
Takami, Masamichi
Yamamoto, Matsuo
Kamijo, Ryutaro
author_sort Imai, Hiroko
collection PubMed
description Monocarboxylate transporters (MCTs) provide transmembrane transport of monocarboxylates such as lactate and pyruvate. The present results showed that α-cyano-4-hydroxycinnamic acid (CHC), an inhibitor of MCTs, promoted osteoclast differentiation from macrophages at lower concentrations (0.1–0.3 mM) and suppressed that at a higher concentration (1.0 mM). On the other hand, CHC reduced the number of mature osteoclasts on the surface of dentin in a concentration-dependent manner. Additionally, macrophages and osteoclasts were found to express the Mct1, Mct2, and Mct4 genes, with Mct1 and Mct4 expression higher in macrophages, and that of Mct2 higher in osteoclasts. Although Mct1 gene knockdown in macrophages enhanced osteoclast formation induced by RANKL, Mct2 gene knockdown suppressed that. Finally, Mct2 gene silencing in mature osteoclasts decreased their number and, thereby, bone resorption. These results suggest that MCT1 is a negative regulator and MCT2 a positive regulator of osteoclast differentiation, while MCT2 is required for bone resorption by osteoclasts.
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spelling pubmed-68217452019-11-05 Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone Imai, Hiroko Yoshimura, Kentaro Miyamoto, Yoichi Sasa, Kiyohito Sugano, Marika Chatani, Masahiro Takami, Masamichi Yamamoto, Matsuo Kamijo, Ryutaro Sci Rep Article Monocarboxylate transporters (MCTs) provide transmembrane transport of monocarboxylates such as lactate and pyruvate. The present results showed that α-cyano-4-hydroxycinnamic acid (CHC), an inhibitor of MCTs, promoted osteoclast differentiation from macrophages at lower concentrations (0.1–0.3 mM) and suppressed that at a higher concentration (1.0 mM). On the other hand, CHC reduced the number of mature osteoclasts on the surface of dentin in a concentration-dependent manner. Additionally, macrophages and osteoclasts were found to express the Mct1, Mct2, and Mct4 genes, with Mct1 and Mct4 expression higher in macrophages, and that of Mct2 higher in osteoclasts. Although Mct1 gene knockdown in macrophages enhanced osteoclast formation induced by RANKL, Mct2 gene knockdown suppressed that. Finally, Mct2 gene silencing in mature osteoclasts decreased their number and, thereby, bone resorption. These results suggest that MCT1 is a negative regulator and MCT2 a positive regulator of osteoclast differentiation, while MCT2 is required for bone resorption by osteoclasts. Nature Publishing Group UK 2019-10-30 /pmc/articles/PMC6821745/ /pubmed/31666601 http://dx.doi.org/10.1038/s41598-019-52128-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Imai, Hiroko
Yoshimura, Kentaro
Miyamoto, Yoichi
Sasa, Kiyohito
Sugano, Marika
Chatani, Masahiro
Takami, Masamichi
Yamamoto, Matsuo
Kamijo, Ryutaro
Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone
title Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone
title_full Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone
title_fullStr Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone
title_full_unstemmed Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone
title_short Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone
title_sort roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821745/
https://www.ncbi.nlm.nih.gov/pubmed/31666601
http://dx.doi.org/10.1038/s41598-019-52128-2
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