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Responses to Quadrivalent Influenza Vaccine Reveal Distinct Circulating CD4+CXCR5+ T Cell Subsets in Men Living with HIV

T cell help for B cells may be perturbed in people living with HIV (PLWH), even when HIV is suppressed, as evidenced by reports of suboptimal responses to influenza vaccination. We investigated cT(FH) responses to the 2017–18 inactivated quadrivalent influenza vaccine (QIV) in men living with antire...

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Autores principales: Cole, Megan E., Saeed, Zainab, Shaw, A. Torm, Guo, Yanping, Höschler, Katja, Winston, Alan, Cooke, Graham S., Fidler, Sarah, Taylor, Graham P., Pollock, Katrina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821795/
https://www.ncbi.nlm.nih.gov/pubmed/31666568
http://dx.doi.org/10.1038/s41598-019-51961-9
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author Cole, Megan E.
Saeed, Zainab
Shaw, A. Torm
Guo, Yanping
Höschler, Katja
Winston, Alan
Cooke, Graham S.
Fidler, Sarah
Taylor, Graham P.
Pollock, Katrina M.
author_facet Cole, Megan E.
Saeed, Zainab
Shaw, A. Torm
Guo, Yanping
Höschler, Katja
Winston, Alan
Cooke, Graham S.
Fidler, Sarah
Taylor, Graham P.
Pollock, Katrina M.
author_sort Cole, Megan E.
collection PubMed
description T cell help for B cells may be perturbed in people living with HIV (PLWH), even when HIV is suppressed, as evidenced by reports of suboptimal responses to influenza vaccination. We investigated cT(FH) responses to the 2017–18 inactivated quadrivalent influenza vaccine (QIV) in men living with antiretroviral therapy (ART)-suppressed HIV infection who were treated in the early or chronic phase of infection, and control subjects. Here we show that seroprotective antibody responses in serum and oral fluid correlated with cT(FH) activation and were equivalent in all three groups, irrespective of when ART was started. These responses were attenuated in those reporting immunisation with influenza vaccine in the preceding three years, independent of HIV infection. Measurement of influenza-specific IgG in oral fluid was closely correlated with haemagglutination inhibition titre. T-SNE and two-dimensional analysis revealed a subset of CD4(+)CXCR3(+)CXCR5(+) cT(FH) activated at one week after vaccination. This was distinguishable from cTFH not activated by vaccination, and a rare, effector memory CD4(+)CXCR5(hi)CD32(hi) T cell subset. The data support the use of QIV for immunisation of PLWH, reveal distinct circulating CD4(+)CXCR5(+) T cell subsets and demonstrate oral fluid sampling for influenza-specific IgG is an alternative to phlebotomy.
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spelling pubmed-68217952019-11-05 Responses to Quadrivalent Influenza Vaccine Reveal Distinct Circulating CD4+CXCR5+ T Cell Subsets in Men Living with HIV Cole, Megan E. Saeed, Zainab Shaw, A. Torm Guo, Yanping Höschler, Katja Winston, Alan Cooke, Graham S. Fidler, Sarah Taylor, Graham P. Pollock, Katrina M. Sci Rep Article T cell help for B cells may be perturbed in people living with HIV (PLWH), even when HIV is suppressed, as evidenced by reports of suboptimal responses to influenza vaccination. We investigated cT(FH) responses to the 2017–18 inactivated quadrivalent influenza vaccine (QIV) in men living with antiretroviral therapy (ART)-suppressed HIV infection who were treated in the early or chronic phase of infection, and control subjects. Here we show that seroprotective antibody responses in serum and oral fluid correlated with cT(FH) activation and were equivalent in all three groups, irrespective of when ART was started. These responses were attenuated in those reporting immunisation with influenza vaccine in the preceding three years, independent of HIV infection. Measurement of influenza-specific IgG in oral fluid was closely correlated with haemagglutination inhibition titre. T-SNE and two-dimensional analysis revealed a subset of CD4(+)CXCR3(+)CXCR5(+) cT(FH) activated at one week after vaccination. This was distinguishable from cTFH not activated by vaccination, and a rare, effector memory CD4(+)CXCR5(hi)CD32(hi) T cell subset. The data support the use of QIV for immunisation of PLWH, reveal distinct circulating CD4(+)CXCR5(+) T cell subsets and demonstrate oral fluid sampling for influenza-specific IgG is an alternative to phlebotomy. Nature Publishing Group UK 2019-10-30 /pmc/articles/PMC6821795/ /pubmed/31666568 http://dx.doi.org/10.1038/s41598-019-51961-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Cole, Megan E.
Saeed, Zainab
Shaw, A. Torm
Guo, Yanping
Höschler, Katja
Winston, Alan
Cooke, Graham S.
Fidler, Sarah
Taylor, Graham P.
Pollock, Katrina M.
Responses to Quadrivalent Influenza Vaccine Reveal Distinct Circulating CD4+CXCR5+ T Cell Subsets in Men Living with HIV
title Responses to Quadrivalent Influenza Vaccine Reveal Distinct Circulating CD4+CXCR5+ T Cell Subsets in Men Living with HIV
title_full Responses to Quadrivalent Influenza Vaccine Reveal Distinct Circulating CD4+CXCR5+ T Cell Subsets in Men Living with HIV
title_fullStr Responses to Quadrivalent Influenza Vaccine Reveal Distinct Circulating CD4+CXCR5+ T Cell Subsets in Men Living with HIV
title_full_unstemmed Responses to Quadrivalent Influenza Vaccine Reveal Distinct Circulating CD4+CXCR5+ T Cell Subsets in Men Living with HIV
title_short Responses to Quadrivalent Influenza Vaccine Reveal Distinct Circulating CD4+CXCR5+ T Cell Subsets in Men Living with HIV
title_sort responses to quadrivalent influenza vaccine reveal distinct circulating cd4+cxcr5+ t cell subsets in men living with hiv
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821795/
https://www.ncbi.nlm.nih.gov/pubmed/31666568
http://dx.doi.org/10.1038/s41598-019-51961-9
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