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Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats

BACKGROUND AND AIMS: Cerium oxide nanoparticles are effective scavengers of reactive oxygen species and have been proposed as a treatment for oxidative stress-related diseases. Consequently, we aimed to investigate the effect of these nanoparticles on hepatic regeneration after liver injury by parti...

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Autores principales: Córdoba-Jover, Bernat, Arce-Cerezo, Altamira, Ribera, Jordi, Pauta, Montse, Oró, Denise, Casals, Gregori, Fernández-Varo, Guillermo, Casals, Eudald, Puntes, Victor, Jiménez, Wladimiro, Morales-Ruiz, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822381/
https://www.ncbi.nlm.nih.gov/pubmed/31672158
http://dx.doi.org/10.1186/s12951-019-0544-5
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author Córdoba-Jover, Bernat
Arce-Cerezo, Altamira
Ribera, Jordi
Pauta, Montse
Oró, Denise
Casals, Gregori
Fernández-Varo, Guillermo
Casals, Eudald
Puntes, Victor
Jiménez, Wladimiro
Morales-Ruiz, Manuel
author_facet Córdoba-Jover, Bernat
Arce-Cerezo, Altamira
Ribera, Jordi
Pauta, Montse
Oró, Denise
Casals, Gregori
Fernández-Varo, Guillermo
Casals, Eudald
Puntes, Victor
Jiménez, Wladimiro
Morales-Ruiz, Manuel
author_sort Córdoba-Jover, Bernat
collection PubMed
description BACKGROUND AND AIMS: Cerium oxide nanoparticles are effective scavengers of reactive oxygen species and have been proposed as a treatment for oxidative stress-related diseases. Consequently, we aimed to investigate the effect of these nanoparticles on hepatic regeneration after liver injury by partial hepatectomy and acetaminophen overdose. METHODS: All the in vitro experiments were performed in HepG2 cells. For the acetaminophen and partial hepatectomy experimental models, male Wistar rats were divided into three groups: (1) nanoparticles group, which received 0.1 mg/kg cerium nanoparticles i.v. twice a week for 2 weeks before 1 g/kg acetaminophen treatment, (2) N-acetyl-cysteine group, which received 300 mg/kg of N-acetyl-cysteine i.p. 1 h after APAP treatment and (3) partial hepatectomy group, which received the same nanoparticles treatment before partial hepatectomy. Each group was matched with vehicle-controlled rats. RESULTS: In the partial hepatectomy model, rats treated with cerium oxide nanoparticles showed a significant increase in liver regeneration, compared with control rats. In the acetaminophen experimental model, nanoparticles and N-acetyl-cysteine treatments decreased early liver damage in hepatic tissue. However, only the effect of cerium oxide nanoparticles was associated with a significant increment in hepatocellular proliferation. This treatment also reduced stress markers and increased cell cycle progression in hepatocytes and the activation of the transcription factor NF-κB in vitro and in vivo. CONCLUSIONS: Our results demonstrate that the nanomaterial cerium oxide, besides their known antioxidant capacities, can enhance hepatocellular proliferation in experimental models of liver regeneration and drug-induced hepatotoxicity.
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spelling pubmed-68223812019-11-06 Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats Córdoba-Jover, Bernat Arce-Cerezo, Altamira Ribera, Jordi Pauta, Montse Oró, Denise Casals, Gregori Fernández-Varo, Guillermo Casals, Eudald Puntes, Victor Jiménez, Wladimiro Morales-Ruiz, Manuel J Nanobiotechnology Research BACKGROUND AND AIMS: Cerium oxide nanoparticles are effective scavengers of reactive oxygen species and have been proposed as a treatment for oxidative stress-related diseases. Consequently, we aimed to investigate the effect of these nanoparticles on hepatic regeneration after liver injury by partial hepatectomy and acetaminophen overdose. METHODS: All the in vitro experiments were performed in HepG2 cells. For the acetaminophen and partial hepatectomy experimental models, male Wistar rats were divided into three groups: (1) nanoparticles group, which received 0.1 mg/kg cerium nanoparticles i.v. twice a week for 2 weeks before 1 g/kg acetaminophen treatment, (2) N-acetyl-cysteine group, which received 300 mg/kg of N-acetyl-cysteine i.p. 1 h after APAP treatment and (3) partial hepatectomy group, which received the same nanoparticles treatment before partial hepatectomy. Each group was matched with vehicle-controlled rats. RESULTS: In the partial hepatectomy model, rats treated with cerium oxide nanoparticles showed a significant increase in liver regeneration, compared with control rats. In the acetaminophen experimental model, nanoparticles and N-acetyl-cysteine treatments decreased early liver damage in hepatic tissue. However, only the effect of cerium oxide nanoparticles was associated with a significant increment in hepatocellular proliferation. This treatment also reduced stress markers and increased cell cycle progression in hepatocytes and the activation of the transcription factor NF-κB in vitro and in vivo. CONCLUSIONS: Our results demonstrate that the nanomaterial cerium oxide, besides their known antioxidant capacities, can enhance hepatocellular proliferation in experimental models of liver regeneration and drug-induced hepatotoxicity. BioMed Central 2019-10-31 /pmc/articles/PMC6822381/ /pubmed/31672158 http://dx.doi.org/10.1186/s12951-019-0544-5 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Córdoba-Jover, Bernat
Arce-Cerezo, Altamira
Ribera, Jordi
Pauta, Montse
Oró, Denise
Casals, Gregori
Fernández-Varo, Guillermo
Casals, Eudald
Puntes, Victor
Jiménez, Wladimiro
Morales-Ruiz, Manuel
Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
title Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
title_full Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
title_fullStr Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
title_full_unstemmed Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
title_short Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
title_sort cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822381/
https://www.ncbi.nlm.nih.gov/pubmed/31672158
http://dx.doi.org/10.1186/s12951-019-0544-5
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