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Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α
Methylation of hypoxia-inducible factor-3α (HIF3A) was previously demonstrated to be highly associated with insulin resistance (IR) in patients with gestational diabetes mellitus (GDM). We aimed to study the therapeutic effects of Berberine (BBR) on GDM and the possible mechanisms. The expressions a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822485/ https://www.ncbi.nlm.nih.gov/pubmed/31652442 http://dx.doi.org/10.1042/BSR20192059 |
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author | Wang, Yuanli Gong, Wenwen Lv, Shaofang Qu, Hongmei He, Yanling |
author_facet | Wang, Yuanli Gong, Wenwen Lv, Shaofang Qu, Hongmei He, Yanling |
author_sort | Wang, Yuanli |
collection | PubMed |
description | Methylation of hypoxia-inducible factor-3α (HIF3A) was previously demonstrated to be highly associated with insulin resistance (IR) in patients with gestational diabetes mellitus (GDM). We aimed to study the therapeutic effects of Berberine (BBR) on GDM and the possible mechanisms. The expressions and methylated states of HIF3A in pregnant women with GDM were compared with that in healthy controls. The IR cell models of 3T3-L1 adipocytes was constructed by 1 μmol/l dexamethasone (Dex) and 1 μmol/l insulin (Ins). To evaluate the effects of BBR on IR adipocyte models, cells were subjected to BBR treatment at different concentrations. Transfection of HIF3A siRNA further confirmed the role of HIF3A in the BBR-induced improving effects. Low expression and high methylation of HIF3A gene were frequent in the GDM pregnancies. BBR treatment noticeably increased the glucose usage rates, adiponectin secretion and cell differentiation of IR 3T3-L1 adipocytes. Increased HIF3A expression and decreased methylated state of HIF3A were also found in IR adipocytes. Furthermore, HIF3A silencing not only reversed the effects of BBR on improving insulin sensibility, but also partially abolished the expression alterations of insulin-related genes in IR adipocytes induced by BBR treatment. Our results suggest that BBR improves insulin sensibility in IR adipocyte models, and the improving effects of BBR are possibly realized through the inhibition of HIF3A methylation. |
format | Online Article Text |
id | pubmed-6822485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68224852019-11-06 Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α Wang, Yuanli Gong, Wenwen Lv, Shaofang Qu, Hongmei He, Yanling Biosci Rep Pharmacology & Toxicology Methylation of hypoxia-inducible factor-3α (HIF3A) was previously demonstrated to be highly associated with insulin resistance (IR) in patients with gestational diabetes mellitus (GDM). We aimed to study the therapeutic effects of Berberine (BBR) on GDM and the possible mechanisms. The expressions and methylated states of HIF3A in pregnant women with GDM were compared with that in healthy controls. The IR cell models of 3T3-L1 adipocytes was constructed by 1 μmol/l dexamethasone (Dex) and 1 μmol/l insulin (Ins). To evaluate the effects of BBR on IR adipocyte models, cells were subjected to BBR treatment at different concentrations. Transfection of HIF3A siRNA further confirmed the role of HIF3A in the BBR-induced improving effects. Low expression and high methylation of HIF3A gene were frequent in the GDM pregnancies. BBR treatment noticeably increased the glucose usage rates, adiponectin secretion and cell differentiation of IR 3T3-L1 adipocytes. Increased HIF3A expression and decreased methylated state of HIF3A were also found in IR adipocytes. Furthermore, HIF3A silencing not only reversed the effects of BBR on improving insulin sensibility, but also partially abolished the expression alterations of insulin-related genes in IR adipocytes induced by BBR treatment. Our results suggest that BBR improves insulin sensibility in IR adipocyte models, and the improving effects of BBR are possibly realized through the inhibition of HIF3A methylation. Portland Press Ltd. 2019-10-25 /pmc/articles/PMC6822485/ /pubmed/31652442 http://dx.doi.org/10.1042/BSR20192059 Text en © 2019 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Pharmacology & Toxicology Wang, Yuanli Gong, Wenwen Lv, Shaofang Qu, Hongmei He, Yanling Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α |
title | Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α |
title_full | Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α |
title_fullStr | Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α |
title_full_unstemmed | Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α |
title_short | Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α |
title_sort | berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α |
topic | Pharmacology & Toxicology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822485/ https://www.ncbi.nlm.nih.gov/pubmed/31652442 http://dx.doi.org/10.1042/BSR20192059 |
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