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Annotation of a hypothetical protein coding gene PAS_chr2-2_0152 containing Lysine Methyl transferase SMYD domain from Komagataella phaffii GS115

The methylotrophic yeast Komagataella phaffii is an industrial workhorse yeast species that has been widely used in biotechnology industries for recombinant protein production. Genome sequencing of this yeast in 2009 have enabled scientists to assign and characterize functions to most of its protein...

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Autores principales: Gogoi, Ridip Kumar, Chorei, Ringhoilal, Prasad, Himanshu Kishore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822524/
https://www.ncbi.nlm.nih.gov/pubmed/31719763
http://dx.doi.org/10.6026/97320630015542
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author Gogoi, Ridip Kumar
Chorei, Ringhoilal
Prasad, Himanshu Kishore
author_facet Gogoi, Ridip Kumar
Chorei, Ringhoilal
Prasad, Himanshu Kishore
author_sort Gogoi, Ridip Kumar
collection PubMed
description The methylotrophic yeast Komagataella phaffii is an industrial workhorse yeast species that has been widely used in biotechnology industries for recombinant protein production. Genome sequencing of this yeast in 2009 have enabled scientists to assign and characterize functions to most of its proteins while few hypothetical proteins remain uncharacterized. Therefore, it is of interest to characterize the hypothetical protein coding gene PAS_chr2-2_0152 as SET containing the ZNF-MYND (SMYD) domain. They share a homology with other methylotrophic and non-methylotrophic yeast species together with known SMYD proteins of Homo sapiens, with conserved distinctive SMYD domain patterns. A homology model is developed using the crystal structure of human histone-lysine methyl transferase smyd3 as template. These data points to that the hypothetical protein is a potential histones and non-histone lysine methyl transferase regulating cell cycle, chromatin remodeling, DNA damage response, homologous recombination and transcription in Komagataella phaffii. Data also suggests the evolutionary syntenic conservation of DNA damage regulator (RFX) and lysine methyl transferase (SMYD) genes in some yeast lineages, pointing to a conserved role requiring further confirmation.
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spelling pubmed-68225242019-11-12 Annotation of a hypothetical protein coding gene PAS_chr2-2_0152 containing Lysine Methyl transferase SMYD domain from Komagataella phaffii GS115 Gogoi, Ridip Kumar Chorei, Ringhoilal Prasad, Himanshu Kishore Bioinformation Research Article The methylotrophic yeast Komagataella phaffii is an industrial workhorse yeast species that has been widely used in biotechnology industries for recombinant protein production. Genome sequencing of this yeast in 2009 have enabled scientists to assign and characterize functions to most of its proteins while few hypothetical proteins remain uncharacterized. Therefore, it is of interest to characterize the hypothetical protein coding gene PAS_chr2-2_0152 as SET containing the ZNF-MYND (SMYD) domain. They share a homology with other methylotrophic and non-methylotrophic yeast species together with known SMYD proteins of Homo sapiens, with conserved distinctive SMYD domain patterns. A homology model is developed using the crystal structure of human histone-lysine methyl transferase smyd3 as template. These data points to that the hypothetical protein is a potential histones and non-histone lysine methyl transferase regulating cell cycle, chromatin remodeling, DNA damage response, homologous recombination and transcription in Komagataella phaffii. Data also suggests the evolutionary syntenic conservation of DNA damage regulator (RFX) and lysine methyl transferase (SMYD) genes in some yeast lineages, pointing to a conserved role requiring further confirmation. Biomedical Informatics 2019-08-31 /pmc/articles/PMC6822524/ /pubmed/31719763 http://dx.doi.org/10.6026/97320630015542 Text en © 2019 Biomedical Informatics http://creativecommons.org/licenses/by/3.0/ This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Article
Gogoi, Ridip Kumar
Chorei, Ringhoilal
Prasad, Himanshu Kishore
Annotation of a hypothetical protein coding gene PAS_chr2-2_0152 containing Lysine Methyl transferase SMYD domain from Komagataella phaffii GS115
title Annotation of a hypothetical protein coding gene PAS_chr2-2_0152 containing Lysine Methyl transferase SMYD domain from Komagataella phaffii GS115
title_full Annotation of a hypothetical protein coding gene PAS_chr2-2_0152 containing Lysine Methyl transferase SMYD domain from Komagataella phaffii GS115
title_fullStr Annotation of a hypothetical protein coding gene PAS_chr2-2_0152 containing Lysine Methyl transferase SMYD domain from Komagataella phaffii GS115
title_full_unstemmed Annotation of a hypothetical protein coding gene PAS_chr2-2_0152 containing Lysine Methyl transferase SMYD domain from Komagataella phaffii GS115
title_short Annotation of a hypothetical protein coding gene PAS_chr2-2_0152 containing Lysine Methyl transferase SMYD domain from Komagataella phaffii GS115
title_sort annotation of a hypothetical protein coding gene pas_chr2-2_0152 containing lysine methyl transferase smyd domain from komagataella phaffii gs115
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822524/
https://www.ncbi.nlm.nih.gov/pubmed/31719763
http://dx.doi.org/10.6026/97320630015542
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