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Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae)
We constructed 13 microsatellite markers for Chrysodeixis includens (Walker), a serious crop pest in the Americas. All SSR (Simple Sequence Repeat) markers were polymorphic, with no evidence of linkage disequilibrium between any loci in any population. The total number of alleles per locus ranged fr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822687/ https://www.ncbi.nlm.nih.gov/pubmed/31671175 http://dx.doi.org/10.1093/jisesa/iez101 |
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author | Silva, C S Cordeiro, E M G Corrêa, A S |
author_facet | Silva, C S Cordeiro, E M G Corrêa, A S |
author_sort | Silva, C S |
collection | PubMed |
description | We constructed 13 microsatellite markers for Chrysodeixis includens (Walker), a serious crop pest in the Americas. All SSR (Simple Sequence Repeat) markers were polymorphic, with no evidence of linkage disequilibrium between any loci in any population. The total number of alleles per locus ranged from 5 for L3, L9, and L11 to 26 for L6; the mean number of alleles per locus in three populations of C. includens ranged from 2.33 for L3 to 14.67 for L6. Hardy–Weinberg equilibrium (HWE) deviation was not observed in four loci for at least one population (L3, L5, L9, L10). Markers L6, L7, L8, L10, L11, L12, and L13 showed a frequency of null alleles > 0.2 for at least one population. STRUCTURE and F-statistics revealed low population structure among the populations (F(ST) = 0.013) and a high degree of inbreeding (F(IS) = 0.658). The SSR markers developed here will be useful in future studies on the ecology, demography, host dynamics, and gene flow of C. includens. This information is essential to understand the recent status of C. includens as a key pest in South America. |
format | Online Article Text |
id | pubmed-6822687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-68226872019-11-04 Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae) Silva, C S Cordeiro, E M G Corrêa, A S J Insect Sci Molecular Entomological Genetics We constructed 13 microsatellite markers for Chrysodeixis includens (Walker), a serious crop pest in the Americas. All SSR (Simple Sequence Repeat) markers were polymorphic, with no evidence of linkage disequilibrium between any loci in any population. The total number of alleles per locus ranged from 5 for L3, L9, and L11 to 26 for L6; the mean number of alleles per locus in three populations of C. includens ranged from 2.33 for L3 to 14.67 for L6. Hardy–Weinberg equilibrium (HWE) deviation was not observed in four loci for at least one population (L3, L5, L9, L10). Markers L6, L7, L8, L10, L11, L12, and L13 showed a frequency of null alleles > 0.2 for at least one population. STRUCTURE and F-statistics revealed low population structure among the populations (F(ST) = 0.013) and a high degree of inbreeding (F(IS) = 0.658). The SSR markers developed here will be useful in future studies on the ecology, demography, host dynamics, and gene flow of C. includens. This information is essential to understand the recent status of C. includens as a key pest in South America. Oxford University Press 2019-10-31 /pmc/articles/PMC6822687/ /pubmed/31671175 http://dx.doi.org/10.1093/jisesa/iez101 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Molecular Entomological Genetics Silva, C S Cordeiro, E M G Corrêa, A S Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae) |
title | Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae) |
title_full | Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae) |
title_fullStr | Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae) |
title_full_unstemmed | Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae) |
title_short | Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae) |
title_sort | isolation and characterization of microsatellite markers for soybean looper (lepidoptera: noctuidae) |
topic | Molecular Entomological Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822687/ https://www.ncbi.nlm.nih.gov/pubmed/31671175 http://dx.doi.org/10.1093/jisesa/iez101 |
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