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Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways

OBJECTIVES: To investigate the role of TLR4/TRAF6/NF-κB pathways in intestinal mucosal injury induced by obstructive jaundice (OJ). METHODS: A total of 100 male C57BL/6J mice were randomly assigned to two groups: (I) sham operation (SH); (II) OJ. The mice were sacrificed before operation and on the...

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Autores principales: Tian, Xiaopeng, Zhao, Huimin, Zhang, Zixuan, Guo, Zengcai, Li, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822728/
https://www.ncbi.nlm.nih.gov/pubmed/31671112
http://dx.doi.org/10.1371/journal.pone.0223651
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author Tian, Xiaopeng
Zhao, Huimin
Zhang, Zixuan
Guo, Zengcai
Li, Wen
author_facet Tian, Xiaopeng
Zhao, Huimin
Zhang, Zixuan
Guo, Zengcai
Li, Wen
author_sort Tian, Xiaopeng
collection PubMed
description OBJECTIVES: To investigate the role of TLR4/TRAF6/NF-κB pathways in intestinal mucosal injury induced by obstructive jaundice (OJ). METHODS: A total of 100 male C57BL/6J mice were randomly assigned to two groups: (I) sham operation (SH); (II) OJ. The mice were sacrificed before operation and on the 1(st), 3(rd), 5(th) and 7(th) day after operation. The blood and terminal ileum were simultaneously collected under the aseptic condition for further detection. RESULTS: In the SH group, TLR4 protein and mRNA rarely expressed in the intestinal mucosa of the mice and there were no significant differences at different time points (p>0.05). By contrast, in the OJ group TLR4 protein (0.12±0.06, 0.16±0.08, 0.27±0.10, 0.35±0.12 and 0.41±0.13, respectively) and mRNA (0.49±0.19, 0.62±0.23, 0.98±0.32, 1.42±0.41 and 1.72±0.49, respectively) increased gradually with the extension of time (p<0.05). Also in the OJ group, the levels of DAO and endotoxin in plasma as well as the expressions of NF-κB and caspase-3 increased gradually with the extension of time, showing positive correlation with the expression of TLR4 (p<0.05). CONCLUSIONS: The expression of TLR4 was significantly up-regulated in the distal ileum of mice with OJ. Activation of the TLR4/TRAF6/NF-κB pathways was involved in the occurrence and development of intestinal mucosal injury and endotoxemia in mice with OJ.
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spelling pubmed-68227282019-11-08 Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways Tian, Xiaopeng Zhao, Huimin Zhang, Zixuan Guo, Zengcai Li, Wen PLoS One Research Article OBJECTIVES: To investigate the role of TLR4/TRAF6/NF-κB pathways in intestinal mucosal injury induced by obstructive jaundice (OJ). METHODS: A total of 100 male C57BL/6J mice were randomly assigned to two groups: (I) sham operation (SH); (II) OJ. The mice were sacrificed before operation and on the 1(st), 3(rd), 5(th) and 7(th) day after operation. The blood and terminal ileum were simultaneously collected under the aseptic condition for further detection. RESULTS: In the SH group, TLR4 protein and mRNA rarely expressed in the intestinal mucosa of the mice and there were no significant differences at different time points (p>0.05). By contrast, in the OJ group TLR4 protein (0.12±0.06, 0.16±0.08, 0.27±0.10, 0.35±0.12 and 0.41±0.13, respectively) and mRNA (0.49±0.19, 0.62±0.23, 0.98±0.32, 1.42±0.41 and 1.72±0.49, respectively) increased gradually with the extension of time (p<0.05). Also in the OJ group, the levels of DAO and endotoxin in plasma as well as the expressions of NF-κB and caspase-3 increased gradually with the extension of time, showing positive correlation with the expression of TLR4 (p<0.05). CONCLUSIONS: The expression of TLR4 was significantly up-regulated in the distal ileum of mice with OJ. Activation of the TLR4/TRAF6/NF-κB pathways was involved in the occurrence and development of intestinal mucosal injury and endotoxemia in mice with OJ. Public Library of Science 2019-10-31 /pmc/articles/PMC6822728/ /pubmed/31671112 http://dx.doi.org/10.1371/journal.pone.0223651 Text en © 2019 Tian et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tian, Xiaopeng
Zhao, Huimin
Zhang, Zixuan
Guo, Zengcai
Li, Wen
Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways
title Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways
title_full Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways
title_fullStr Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways
title_full_unstemmed Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways
title_short Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways
title_sort intestinal mucosal injury induced by obstructive jaundice is associated with activation of tlr4/traf6/nf-κb pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822728/
https://www.ncbi.nlm.nih.gov/pubmed/31671112
http://dx.doi.org/10.1371/journal.pone.0223651
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