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De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns

The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves and oscillatory patterns. In the context of developing a synt...

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Autores principales: Godino, Elisa, López, Jonás Noguera, Foschepoth, David, Cleij, Céline, Doerr, Anne, Castellà, Clara Ferrer, Danelon, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823393/
https://www.ncbi.nlm.nih.gov/pubmed/31672986
http://dx.doi.org/10.1038/s41467-019-12932-w
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author Godino, Elisa
López, Jonás Noguera
Foschepoth, David
Cleij, Céline
Doerr, Anne
Castellà, Clara Ferrer
Danelon, Christophe
author_facet Godino, Elisa
López, Jonás Noguera
Foschepoth, David
Cleij, Céline
Doerr, Anne
Castellà, Clara Ferrer
Danelon, Christophe
author_sort Godino, Elisa
collection PubMed
description The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves and oscillatory patterns. In the context of developing a synthetic cell from elementary biological modules, harnessing Min oscillations might allow us to implement higher-order cellular functions. To convey hereditary information, the Min system must be encoded in a DNA molecule that can be copied, transcribed, and translated. Here, the MinD and MinE proteins are synthesized de novo from their genes inside liposomes. Dynamic protein patterns and accompanying liposome shape deformation are observed. When integrated with the cytoskeletal proteins FtsA and FtsZ, the synthetic Min system is able to dynamically regulate FtsZ patterns. By enabling genetic control over Min protein self-organization and membrane remodeling, our methodology offers unique opportunities towards directed evolution of bacterial division processes in vitro.
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spelling pubmed-68233932019-11-04 De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns Godino, Elisa López, Jonás Noguera Foschepoth, David Cleij, Céline Doerr, Anne Castellà, Clara Ferrer Danelon, Christophe Nat Commun Article The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves and oscillatory patterns. In the context of developing a synthetic cell from elementary biological modules, harnessing Min oscillations might allow us to implement higher-order cellular functions. To convey hereditary information, the Min system must be encoded in a DNA molecule that can be copied, transcribed, and translated. Here, the MinD and MinE proteins are synthesized de novo from their genes inside liposomes. Dynamic protein patterns and accompanying liposome shape deformation are observed. When integrated with the cytoskeletal proteins FtsA and FtsZ, the synthetic Min system is able to dynamically regulate FtsZ patterns. By enabling genetic control over Min protein self-organization and membrane remodeling, our methodology offers unique opportunities towards directed evolution of bacterial division processes in vitro. Nature Publishing Group UK 2019-10-31 /pmc/articles/PMC6823393/ /pubmed/31672986 http://dx.doi.org/10.1038/s41467-019-12932-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Godino, Elisa
López, Jonás Noguera
Foschepoth, David
Cleij, Céline
Doerr, Anne
Castellà, Clara Ferrer
Danelon, Christophe
De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
title De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
title_full De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
title_fullStr De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
title_full_unstemmed De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
title_short De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
title_sort de novo synthesized min proteins drive oscillatory liposome deformation and regulate ftsa-ftsz cytoskeletal patterns
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823393/
https://www.ncbi.nlm.nih.gov/pubmed/31672986
http://dx.doi.org/10.1038/s41467-019-12932-w
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