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Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein

A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)...

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Autores principales: Park, Kyung Mi, Chung, Da Jung, Choi, Mijin, Kang, Taejoon, Jeong, Jinyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823421/
https://www.ncbi.nlm.nih.gov/pubmed/31673811
http://dx.doi.org/10.1186/s40580-019-0207-0
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author Park, Kyung Mi
Chung, Da Jung
Choi, Mijin
Kang, Taejoon
Jeong, Jinyoung
author_facet Park, Kyung Mi
Chung, Da Jung
Choi, Mijin
Kang, Taejoon
Jeong, Jinyoung
author_sort Park, Kyung Mi
collection PubMed
description A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.
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spelling pubmed-68234212019-11-14 Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein Park, Kyung Mi Chung, Da Jung Choi, Mijin Kang, Taejoon Jeong, Jinyoung Nano Converg Research A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety. Springer Singapore 2019-11-01 /pmc/articles/PMC6823421/ /pubmed/31673811 http://dx.doi.org/10.1186/s40580-019-0207-0 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Park, Kyung Mi
Chung, Da Jung
Choi, Mijin
Kang, Taejoon
Jeong, Jinyoung
Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein
title Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein
title_full Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein
title_fullStr Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein
title_full_unstemmed Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein
title_short Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein
title_sort fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of c-reactive protein
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823421/
https://www.ncbi.nlm.nih.gov/pubmed/31673811
http://dx.doi.org/10.1186/s40580-019-0207-0
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