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Using slow frame rate imaging to extract fast receptive fields

In functional imaging, large numbers of neurons are measured during sensory stimulation or behavior. This data can be used to map receptive fields that describe neural associations with stimuli or with behavior. The temporal resolution of these receptive fields has traditionally been limited by imag...

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Autores principales: Mano, Omer, Creamer, Matthew S., Matulis, Catherine A., Salazar-Gatzimas, Emilio, Chen, Juyue, Zavatone-Veth, Jacob A., Clark, Damon A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823504/
https://www.ncbi.nlm.nih.gov/pubmed/31672963
http://dx.doi.org/10.1038/s41467-019-12974-0
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author Mano, Omer
Creamer, Matthew S.
Matulis, Catherine A.
Salazar-Gatzimas, Emilio
Chen, Juyue
Zavatone-Veth, Jacob A.
Clark, Damon A.
author_facet Mano, Omer
Creamer, Matthew S.
Matulis, Catherine A.
Salazar-Gatzimas, Emilio
Chen, Juyue
Zavatone-Veth, Jacob A.
Clark, Damon A.
author_sort Mano, Omer
collection PubMed
description In functional imaging, large numbers of neurons are measured during sensory stimulation or behavior. This data can be used to map receptive fields that describe neural associations with stimuli or with behavior. The temporal resolution of these receptive fields has traditionally been limited by image acquisition rates. However, even when acquisitions scan slowly across a population of neurons, individual neurons may be measured at precisely known times. Here, we apply a method that leverages the timing of neural measurements to find receptive fields with temporal resolutions higher than the image acquisition rate. We use this temporal super-resolution method to resolve fast voltage and glutamate responses in visual neurons in Drosophila and to extract calcium receptive fields from cortical neurons in mammals. We provide code to easily apply this method to existing datasets. This method requires no specialized hardware and can be used with any optical indicator of neural activity.
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spelling pubmed-68235042019-11-04 Using slow frame rate imaging to extract fast receptive fields Mano, Omer Creamer, Matthew S. Matulis, Catherine A. Salazar-Gatzimas, Emilio Chen, Juyue Zavatone-Veth, Jacob A. Clark, Damon A. Nat Commun Article In functional imaging, large numbers of neurons are measured during sensory stimulation or behavior. This data can be used to map receptive fields that describe neural associations with stimuli or with behavior. The temporal resolution of these receptive fields has traditionally been limited by image acquisition rates. However, even when acquisitions scan slowly across a population of neurons, individual neurons may be measured at precisely known times. Here, we apply a method that leverages the timing of neural measurements to find receptive fields with temporal resolutions higher than the image acquisition rate. We use this temporal super-resolution method to resolve fast voltage and glutamate responses in visual neurons in Drosophila and to extract calcium receptive fields from cortical neurons in mammals. We provide code to easily apply this method to existing datasets. This method requires no specialized hardware and can be used with any optical indicator of neural activity. Nature Publishing Group UK 2019-10-31 /pmc/articles/PMC6823504/ /pubmed/31672963 http://dx.doi.org/10.1038/s41467-019-12974-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mano, Omer
Creamer, Matthew S.
Matulis, Catherine A.
Salazar-Gatzimas, Emilio
Chen, Juyue
Zavatone-Veth, Jacob A.
Clark, Damon A.
Using slow frame rate imaging to extract fast receptive fields
title Using slow frame rate imaging to extract fast receptive fields
title_full Using slow frame rate imaging to extract fast receptive fields
title_fullStr Using slow frame rate imaging to extract fast receptive fields
title_full_unstemmed Using slow frame rate imaging to extract fast receptive fields
title_short Using slow frame rate imaging to extract fast receptive fields
title_sort using slow frame rate imaging to extract fast receptive fields
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823504/
https://www.ncbi.nlm.nih.gov/pubmed/31672963
http://dx.doi.org/10.1038/s41467-019-12974-0
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