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The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death

OBJECTIVE: Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of Viola odorata L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of...

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Autores principales: Tayarani-Najaran, Zahra, Yazdian-Robati, Rezvan, Amini, Elaheh, Salek, Farzaneh, Arasteh, Fatemeh, Emami, Seyed Ahmad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823524/
https://www.ncbi.nlm.nih.gov/pubmed/31763208
http://dx.doi.org/10.22038/AJP.2019.13098
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author Tayarani-Najaran, Zahra
Yazdian-Robati, Rezvan
Amini, Elaheh
Salek, Farzaneh
Arasteh, Fatemeh
Emami, Seyed Ahmad
author_facet Tayarani-Najaran, Zahra
Yazdian-Robati, Rezvan
Amini, Elaheh
Salek, Farzaneh
Arasteh, Fatemeh
Emami, Seyed Ahmad
author_sort Tayarani-Najaran, Zahra
collection PubMed
description OBJECTIVE: Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of Viola odorata L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of methanol (MeOH) extract and other fractions isolated from V. odorata in PC12 cell line in serum/glucose deprivation (SGD) condition. MATERIALS AND METHODS: The PC12 neuronal cells were pretreated for 6 hr with MeOH extract and fractions of V. odorata (1 to 25 μg/ml) followed by 24 hr incubation under SGD condition. Cell viability was measured by Alamar Blue(® )assay. The level of ROS was calculated using DCFH-DA. Also, Bax/Bcl-2 protein ratio was analyzed by western blot assay. RESULTS: SGD condition significantly decreased cells viability (p<0.001). Pretreatment with EtOAc (12.5 and 25 µg/ml), BuOH (12, 25, 50 µg/ml) and CH(2)Cl(2 )(1.5 µg/ml) fractions of V. odorata reduced SGD-induced cytotoxicity. MeOH extract could not increase the viability significantly. All four semi polar fractions (EtOAc, BuOH, CH2Cl2 and MeOH) decreased SGD-induced ROS production and changed Bax/Bcl-2 ratio. CONCLUSION: V. odorata showed promising effects against SGD condition; further mechanistic and clinical studies are warranted before application of V. odorata as a neuro-protective agent.
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spelling pubmed-68235242019-11-22 The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death Tayarani-Najaran, Zahra Yazdian-Robati, Rezvan Amini, Elaheh Salek, Farzaneh Arasteh, Fatemeh Emami, Seyed Ahmad Avicenna J Phytomed Short Communication OBJECTIVE: Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of Viola odorata L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of methanol (MeOH) extract and other fractions isolated from V. odorata in PC12 cell line in serum/glucose deprivation (SGD) condition. MATERIALS AND METHODS: The PC12 neuronal cells were pretreated for 6 hr with MeOH extract and fractions of V. odorata (1 to 25 μg/ml) followed by 24 hr incubation under SGD condition. Cell viability was measured by Alamar Blue(® )assay. The level of ROS was calculated using DCFH-DA. Also, Bax/Bcl-2 protein ratio was analyzed by western blot assay. RESULTS: SGD condition significantly decreased cells viability (p<0.001). Pretreatment with EtOAc (12.5 and 25 µg/ml), BuOH (12, 25, 50 µg/ml) and CH(2)Cl(2 )(1.5 µg/ml) fractions of V. odorata reduced SGD-induced cytotoxicity. MeOH extract could not increase the viability significantly. All four semi polar fractions (EtOAc, BuOH, CH2Cl2 and MeOH) decreased SGD-induced ROS production and changed Bax/Bcl-2 ratio. CONCLUSION: V. odorata showed promising effects against SGD condition; further mechanistic and clinical studies are warranted before application of V. odorata as a neuro-protective agent. Mashhad University of Medical Sciences 2019 /pmc/articles/PMC6823524/ /pubmed/31763208 http://dx.doi.org/10.22038/AJP.2019.13098 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Tayarani-Najaran, Zahra
Yazdian-Robati, Rezvan
Amini, Elaheh
Salek, Farzaneh
Arasteh, Fatemeh
Emami, Seyed Ahmad
The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
title The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
title_full The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
title_fullStr The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
title_full_unstemmed The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
title_short The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
title_sort mechanism of neuroprotective effect of viola odorata against serum/glucose deprivation-induced pc12 cell death
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823524/
https://www.ncbi.nlm.nih.gov/pubmed/31763208
http://dx.doi.org/10.22038/AJP.2019.13098
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