Cargando…
The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
OBJECTIVE: Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of Viola odorata L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823524/ https://www.ncbi.nlm.nih.gov/pubmed/31763208 http://dx.doi.org/10.22038/AJP.2019.13098 |
_version_ | 1783464548850728960 |
---|---|
author | Tayarani-Najaran, Zahra Yazdian-Robati, Rezvan Amini, Elaheh Salek, Farzaneh Arasteh, Fatemeh Emami, Seyed Ahmad |
author_facet | Tayarani-Najaran, Zahra Yazdian-Robati, Rezvan Amini, Elaheh Salek, Farzaneh Arasteh, Fatemeh Emami, Seyed Ahmad |
author_sort | Tayarani-Najaran, Zahra |
collection | PubMed |
description | OBJECTIVE: Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of Viola odorata L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of methanol (MeOH) extract and other fractions isolated from V. odorata in PC12 cell line in serum/glucose deprivation (SGD) condition. MATERIALS AND METHODS: The PC12 neuronal cells were pretreated for 6 hr with MeOH extract and fractions of V. odorata (1 to 25 μg/ml) followed by 24 hr incubation under SGD condition. Cell viability was measured by Alamar Blue(® )assay. The level of ROS was calculated using DCFH-DA. Also, Bax/Bcl-2 protein ratio was analyzed by western blot assay. RESULTS: SGD condition significantly decreased cells viability (p<0.001). Pretreatment with EtOAc (12.5 and 25 µg/ml), BuOH (12, 25, 50 µg/ml) and CH(2)Cl(2 )(1.5 µg/ml) fractions of V. odorata reduced SGD-induced cytotoxicity. MeOH extract could not increase the viability significantly. All four semi polar fractions (EtOAc, BuOH, CH2Cl2 and MeOH) decreased SGD-induced ROS production and changed Bax/Bcl-2 ratio. CONCLUSION: V. odorata showed promising effects against SGD condition; further mechanistic and clinical studies are warranted before application of V. odorata as a neuro-protective agent. |
format | Online Article Text |
id | pubmed-6823524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-68235242019-11-22 The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death Tayarani-Najaran, Zahra Yazdian-Robati, Rezvan Amini, Elaheh Salek, Farzaneh Arasteh, Fatemeh Emami, Seyed Ahmad Avicenna J Phytomed Short Communication OBJECTIVE: Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of Viola odorata L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of methanol (MeOH) extract and other fractions isolated from V. odorata in PC12 cell line in serum/glucose deprivation (SGD) condition. MATERIALS AND METHODS: The PC12 neuronal cells were pretreated for 6 hr with MeOH extract and fractions of V. odorata (1 to 25 μg/ml) followed by 24 hr incubation under SGD condition. Cell viability was measured by Alamar Blue(® )assay. The level of ROS was calculated using DCFH-DA. Also, Bax/Bcl-2 protein ratio was analyzed by western blot assay. RESULTS: SGD condition significantly decreased cells viability (p<0.001). Pretreatment with EtOAc (12.5 and 25 µg/ml), BuOH (12, 25, 50 µg/ml) and CH(2)Cl(2 )(1.5 µg/ml) fractions of V. odorata reduced SGD-induced cytotoxicity. MeOH extract could not increase the viability significantly. All four semi polar fractions (EtOAc, BuOH, CH2Cl2 and MeOH) decreased SGD-induced ROS production and changed Bax/Bcl-2 ratio. CONCLUSION: V. odorata showed promising effects against SGD condition; further mechanistic and clinical studies are warranted before application of V. odorata as a neuro-protective agent. Mashhad University of Medical Sciences 2019 /pmc/articles/PMC6823524/ /pubmed/31763208 http://dx.doi.org/10.22038/AJP.2019.13098 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Tayarani-Najaran, Zahra Yazdian-Robati, Rezvan Amini, Elaheh Salek, Farzaneh Arasteh, Fatemeh Emami, Seyed Ahmad The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death |
title | The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death |
title_full | The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death |
title_fullStr | The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death |
title_full_unstemmed | The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death |
title_short | The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death |
title_sort | mechanism of neuroprotective effect of viola odorata against serum/glucose deprivation-induced pc12 cell death |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823524/ https://www.ncbi.nlm.nih.gov/pubmed/31763208 http://dx.doi.org/10.22038/AJP.2019.13098 |
work_keys_str_mv | AT tayaraninajaranzahra themechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT yazdianrobatirezvan themechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT aminielaheh themechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT salekfarzaneh themechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT arastehfatemeh themechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT emamiseyedahmad themechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT tayaraninajaranzahra mechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT yazdianrobatirezvan mechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT aminielaheh mechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT salekfarzaneh mechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT arastehfatemeh mechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath AT emamiseyedahmad mechanismofneuroprotectiveeffectofviolaodorataagainstserumglucosedeprivationinducedpc12celldeath |