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Auxin regulation involved in gynoecium morphogenesis of papaya flowers

The morphogenesis of gynoecium is crucial for propagation and productivity of fruit crops. For trioecious papaya (Carica papaya), highly differentiated morphology of gynoecium in flowers of different sex types is controlled by gene networks and influenced by environmental factors, but the regulatory...

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Autores principales: Zhou, Ping, Fatima, Mahpara, Ma, Xinyi, Liu, Juan, Ming, Ray
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823548/
https://www.ncbi.nlm.nih.gov/pubmed/31700646
http://dx.doi.org/10.1038/s41438-019-0205-8
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author Zhou, Ping
Fatima, Mahpara
Ma, Xinyi
Liu, Juan
Ming, Ray
author_facet Zhou, Ping
Fatima, Mahpara
Ma, Xinyi
Liu, Juan
Ming, Ray
author_sort Zhou, Ping
collection PubMed
description The morphogenesis of gynoecium is crucial for propagation and productivity of fruit crops. For trioecious papaya (Carica papaya), highly differentiated morphology of gynoecium in flowers of different sex types is controlled by gene networks and influenced by environmental factors, but the regulatory mechanism in gynoecium morphogenesis is unclear. Gynodioecious and dioecious papaya varieties were used for analysis of differentially expressed genes followed by experiments using auxin and an auxin transporter inhibitor. We first compared differential gene expression in functional and rudimentary gynoecium at early stage of their development and detected significant difference in phytohormone modulating and transduction processes, particularly auxin. Enhanced auxin signal transduction in rudimentary gynoecium was observed. To determine the role auxin plays in the papaya gynoecium, auxin transport inhibitor (N-1-Naphthylphthalamic acid, NPA) and synthetic auxin analogs with different concentrations gradient were sprayed to the trunk apex of male and female plants of dioecious papaya. Weakening of auxin transport by 10 mg/L NPA treatment resulted in female fertility restoration in male flowers, while female flowers did not show changes. NPA treatment with higher concentration (30 and 50 mg/L) caused deformed flowers in both male and female plants. We hypothesize that the occurrence of rudimentary gynoecium patterning might associate with auxin homeostasis alteration. Proper auxin concentration and auxin homeostasis might be crucial for functional gynoecium morphogenesis in papaya flowers. These results will lead to further investigation on the auxin homeostasis and gynoecium morphogenesis in papaya.
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spelling pubmed-68235482019-11-07 Auxin regulation involved in gynoecium morphogenesis of papaya flowers Zhou, Ping Fatima, Mahpara Ma, Xinyi Liu, Juan Ming, Ray Hortic Res Article The morphogenesis of gynoecium is crucial for propagation and productivity of fruit crops. For trioecious papaya (Carica papaya), highly differentiated morphology of gynoecium in flowers of different sex types is controlled by gene networks and influenced by environmental factors, but the regulatory mechanism in gynoecium morphogenesis is unclear. Gynodioecious and dioecious papaya varieties were used for analysis of differentially expressed genes followed by experiments using auxin and an auxin transporter inhibitor. We first compared differential gene expression in functional and rudimentary gynoecium at early stage of their development and detected significant difference in phytohormone modulating and transduction processes, particularly auxin. Enhanced auxin signal transduction in rudimentary gynoecium was observed. To determine the role auxin plays in the papaya gynoecium, auxin transport inhibitor (N-1-Naphthylphthalamic acid, NPA) and synthetic auxin analogs with different concentrations gradient were sprayed to the trunk apex of male and female plants of dioecious papaya. Weakening of auxin transport by 10 mg/L NPA treatment resulted in female fertility restoration in male flowers, while female flowers did not show changes. NPA treatment with higher concentration (30 and 50 mg/L) caused deformed flowers in both male and female plants. We hypothesize that the occurrence of rudimentary gynoecium patterning might associate with auxin homeostasis alteration. Proper auxin concentration and auxin homeostasis might be crucial for functional gynoecium morphogenesis in papaya flowers. These results will lead to further investigation on the auxin homeostasis and gynoecium morphogenesis in papaya. Nature Publishing Group UK 2019-11-01 /pmc/articles/PMC6823548/ /pubmed/31700646 http://dx.doi.org/10.1038/s41438-019-0205-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhou, Ping
Fatima, Mahpara
Ma, Xinyi
Liu, Juan
Ming, Ray
Auxin regulation involved in gynoecium morphogenesis of papaya flowers
title Auxin regulation involved in gynoecium morphogenesis of papaya flowers
title_full Auxin regulation involved in gynoecium morphogenesis of papaya flowers
title_fullStr Auxin regulation involved in gynoecium morphogenesis of papaya flowers
title_full_unstemmed Auxin regulation involved in gynoecium morphogenesis of papaya flowers
title_short Auxin regulation involved in gynoecium morphogenesis of papaya flowers
title_sort auxin regulation involved in gynoecium morphogenesis of papaya flowers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823548/
https://www.ncbi.nlm.nih.gov/pubmed/31700646
http://dx.doi.org/10.1038/s41438-019-0205-8
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