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Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress

Kandelia candel is one of the mangrove species that are most resistant to environmental stress. As a typical nonsalt-secreting mangrove plant, K. candel is an ideal biological material to analyze the molecular mechanism of salt tolerance in woody plants. In this study, changes in protein abundance a...

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Autores principales: XING, Jianhong, PAN, Dezhuo, WANG, Lingxia, TAN, Fanglin, CHEN, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific and Technological Research Council of Turkey 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823913/
https://www.ncbi.nlm.nih.gov/pubmed/31768104
http://dx.doi.org/10.3906/biy-1906-22
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author XING, Jianhong
PAN, Dezhuo
WANG, Lingxia
TAN, Fanglin
CHEN, Wei
author_facet XING, Jianhong
PAN, Dezhuo
WANG, Lingxia
TAN, Fanglin
CHEN, Wei
author_sort XING, Jianhong
collection PubMed
description Kandelia candel is one of the mangrove species that are most resistant to environmental stress. As a typical nonsalt-secreting mangrove plant, K. candel is an ideal biological material to analyze the molecular mechanism of salt tolerance in woody plants. In this study, changes in protein abundance and expression profile in K. candel roots under high-salinity stress of 600 mmol L(-1) NaCl were analyzed using isobaric tags for relative and absolute quantification (iTRAQ) assay. Moreover, the physiological parameters associated with metabolic pathways in which the differentially abundant proteins (DAPs) are involved were determined. A total of 5577 proteins were identified by iTRAQ analysis of the K. candel root proteins, of which 227 were DAPs with a fold change ratio >1.2 or a fold change ratio <0.83 and a P-value <0.05. A total of 227 DAPs consisting of 110 up-regulated and 117 down-regulated proteins were identified. Our Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the DAPs were primarily involved in biological processes including carbohydrate and energy metabolisms, stress response and defense, cell wall structure, and secondary metabolism. The results of the physiological parameters showed that their profile changes were consistent with those of the proteome analysis. The results of the proteome and physiological parameters showed that K. candel roots could resist high-salinity stress by maintaining a normal Embden-Meyerhof-Parnas and tricarboxylic acid (EMP-TCA) pathway, increasing the activities of various antioxidant enzymes and antioxidant contents, stabilizing the cell wall structure, and accumulating secondary metabolites such as triterpenoids.
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spelling pubmed-68239132019-11-25 Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress XING, Jianhong PAN, Dezhuo WANG, Lingxia TAN, Fanglin CHEN, Wei Turk J Biol Article Kandelia candel is one of the mangrove species that are most resistant to environmental stress. As a typical nonsalt-secreting mangrove plant, K. candel is an ideal biological material to analyze the molecular mechanism of salt tolerance in woody plants. In this study, changes in protein abundance and expression profile in K. candel roots under high-salinity stress of 600 mmol L(-1) NaCl were analyzed using isobaric tags for relative and absolute quantification (iTRAQ) assay. Moreover, the physiological parameters associated with metabolic pathways in which the differentially abundant proteins (DAPs) are involved were determined. A total of 5577 proteins were identified by iTRAQ analysis of the K. candel root proteins, of which 227 were DAPs with a fold change ratio >1.2 or a fold change ratio <0.83 and a P-value <0.05. A total of 227 DAPs consisting of 110 up-regulated and 117 down-regulated proteins were identified. Our Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the DAPs were primarily involved in biological processes including carbohydrate and energy metabolisms, stress response and defense, cell wall structure, and secondary metabolism. The results of the physiological parameters showed that their profile changes were consistent with those of the proteome analysis. The results of the proteome and physiological parameters showed that K. candel roots could resist high-salinity stress by maintaining a normal Embden-Meyerhof-Parnas and tricarboxylic acid (EMP-TCA) pathway, increasing the activities of various antioxidant enzymes and antioxidant contents, stabilizing the cell wall structure, and accumulating secondary metabolites such as triterpenoids. The Scientific and Technological Research Council of Turkey 2019-10-14 /pmc/articles/PMC6823913/ /pubmed/31768104 http://dx.doi.org/10.3906/biy-1906-22 Text en Copyright © 2019 The Author(s) This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Article
XING, Jianhong
PAN, Dezhuo
WANG, Lingxia
TAN, Fanglin
CHEN, Wei
Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress
title Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress
title_full Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress
title_fullStr Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress
title_full_unstemmed Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress
title_short Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress
title_sort proteomic and physiological responses in mangrove kandelia candel roots under short-term high-salinity stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823913/
https://www.ncbi.nlm.nih.gov/pubmed/31768104
http://dx.doi.org/10.3906/biy-1906-22
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