Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures

OBJECTIVE: We aim to directly detect Mycoplasma DNA in a U937 suspension cell culture without using DNA purification. In order to make Mycoplasma contamination monitoring easier, we optimized a commercially available quantitative PCR (qPCR)-based detection kit. We compared the sensitivity of direct...

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Detalles Bibliográficos
Autores principales: Baaity, Zain, Breunig, Sven, Önder, Kamil, Somogyvári, Ferenc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823952/
https://www.ncbi.nlm.nih.gov/pubmed/31675990
http://dx.doi.org/10.1186/s13104-019-4763-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823952/
https://www.ncbi.nlm.nih.gov/pubmed/31675990
http://dx.doi.org/10.1186/s13104-019-4763-5

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