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Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures
OBJECTIVE: We aim to directly detect Mycoplasma DNA in a U937 suspension cell culture without using DNA purification. In order to make Mycoplasma contamination monitoring easier, we optimized a commercially available quantitative PCR (qPCR)-based detection kit. We compared the sensitivity of direct...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823952/ https://www.ncbi.nlm.nih.gov/pubmed/31675990 http://dx.doi.org/10.1186/s13104-019-4763-5 |