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Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury

OBJECTIVES: Primary cilia are sensory organelles which co-ordinate several developmental/repair pathways including hedgehog signalling. Studies of human renal allografts suffering acute tubular necrosis have shown that length of primary cilia borne by epithelial cells doubles throughout the nephron...

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Autores principales: Verghese, Elizabeth, Martelotto, Luciano G., Cain, Jason E., Williams, Timothy M., Wise, Andrea F., Hill, Prudence A., Langham, Robyn G., Watkins, D. Neil, Ricardo, Sharon D., Deane, James A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824085/
https://www.ncbi.nlm.nih.gov/pubmed/31676011
http://dx.doi.org/10.1186/s13104-019-4738-6
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author Verghese, Elizabeth
Martelotto, Luciano G.
Cain, Jason E.
Williams, Timothy M.
Wise, Andrea F.
Hill, Prudence A.
Langham, Robyn G.
Watkins, D. Neil
Ricardo, Sharon D.
Deane, James A.
author_facet Verghese, Elizabeth
Martelotto, Luciano G.
Cain, Jason E.
Williams, Timothy M.
Wise, Andrea F.
Hill, Prudence A.
Langham, Robyn G.
Watkins, D. Neil
Ricardo, Sharon D.
Deane, James A.
author_sort Verghese, Elizabeth
collection PubMed
description OBJECTIVES: Primary cilia are sensory organelles which co-ordinate several developmental/repair pathways including hedgehog signalling. Studies of human renal allografts suffering acute tubular necrosis have shown that length of primary cilia borne by epithelial cells doubles throughout the nephron and collecting duct, and then normalises as renal function returns. Conversely the loss of primary cilia has been reported in chronic allograft rejection and linked to defective hedgehog signalling. We investigated the fate of primary cilia in renal allografts suffering acute rejection. RESULTS: Here we observed that in renal allografts undergoing acute rejection, primary cilia were retained, with their length increasing 1 week after transplantation and remaining elevated. We used a mouse model of acute renal injury to demonstrate that elongated renal primary cilia in the injured renal tubule show evidence of smoothened accumulation, a biomarker for activation of hedgehog signalling. We conclude that primary cilium-mediated activation of hedgehog signalling is still possible during the acute phase of renal allograft rejection.
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spelling pubmed-68240852019-11-06 Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury Verghese, Elizabeth Martelotto, Luciano G. Cain, Jason E. Williams, Timothy M. Wise, Andrea F. Hill, Prudence A. Langham, Robyn G. Watkins, D. Neil Ricardo, Sharon D. Deane, James A. BMC Res Notes Research Note OBJECTIVES: Primary cilia are sensory organelles which co-ordinate several developmental/repair pathways including hedgehog signalling. Studies of human renal allografts suffering acute tubular necrosis have shown that length of primary cilia borne by epithelial cells doubles throughout the nephron and collecting duct, and then normalises as renal function returns. Conversely the loss of primary cilia has been reported in chronic allograft rejection and linked to defective hedgehog signalling. We investigated the fate of primary cilia in renal allografts suffering acute rejection. RESULTS: Here we observed that in renal allografts undergoing acute rejection, primary cilia were retained, with their length increasing 1 week after transplantation and remaining elevated. We used a mouse model of acute renal injury to demonstrate that elongated renal primary cilia in the injured renal tubule show evidence of smoothened accumulation, a biomarker for activation of hedgehog signalling. We conclude that primary cilium-mediated activation of hedgehog signalling is still possible during the acute phase of renal allograft rejection. BioMed Central 2019-11-01 /pmc/articles/PMC6824085/ /pubmed/31676011 http://dx.doi.org/10.1186/s13104-019-4738-6 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Verghese, Elizabeth
Martelotto, Luciano G.
Cain, Jason E.
Williams, Timothy M.
Wise, Andrea F.
Hill, Prudence A.
Langham, Robyn G.
Watkins, D. Neil
Ricardo, Sharon D.
Deane, James A.
Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury
title Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury
title_full Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury
title_fullStr Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury
title_full_unstemmed Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury
title_short Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury
title_sort renal epithelial cells retain primary cilia during human acute renal allograft rejection injury
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824085/
https://www.ncbi.nlm.nih.gov/pubmed/31676011
http://dx.doi.org/10.1186/s13104-019-4738-6
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