Cargando…

Integrative proteomic and phosphoproteomic profiling of prostate cell lines

BACKGROUND: Prostate cancer is a major public health issue, mainly because patients relapse after androgen deprivation therapy. Proteomic strategies, aiming to reflect the functional activity of cells, are nowadays among the leading approaches to tackle the challenges not only of better diagnosis, b...

Descripción completa

Detalles Bibliográficos
Autores principales: Katsogiannou, Maria, Boyer, Jean-Baptiste, Valdeolivas, Alberto, Remy, Elisabeth, Calzone, Laurence, Audebert, Stéphane, Rocchi, Palma, Camoin, Luc, Baudot, Anaïs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824562/
https://www.ncbi.nlm.nih.gov/pubmed/31675377
http://dx.doi.org/10.1371/journal.pone.0224148
_version_ 1783464752431759360
author Katsogiannou, Maria
Boyer, Jean-Baptiste
Valdeolivas, Alberto
Remy, Elisabeth
Calzone, Laurence
Audebert, Stéphane
Rocchi, Palma
Camoin, Luc
Baudot, Anaïs
author_facet Katsogiannou, Maria
Boyer, Jean-Baptiste
Valdeolivas, Alberto
Remy, Elisabeth
Calzone, Laurence
Audebert, Stéphane
Rocchi, Palma
Camoin, Luc
Baudot, Anaïs
author_sort Katsogiannou, Maria
collection PubMed
description BACKGROUND: Prostate cancer is a major public health issue, mainly because patients relapse after androgen deprivation therapy. Proteomic strategies, aiming to reflect the functional activity of cells, are nowadays among the leading approaches to tackle the challenges not only of better diagnosis, but also of unraveling mechanistic details related to disease etiology and progression. METHODS: We conducted here a large SILAC-based Mass Spectrometry experiment to map the proteomes and phosphoproteomes of four widely used prostate cell lines, namely PNT1A, LNCaP, DU145 and PC3, representative of different cancerous and hormonal status. RESULTS: We identified more than 3000 proteins and phosphosites, from which we quantified more than 1000 proteins and 500 phosphosites after stringent filtering. Extensive exploration of this proteomics and phosphoproteomics dataset allowed characterizing housekeeping as well as cell-line specific proteins, phosphosites and functional features of each cell line. In addition, by comparing the sensitive and resistant cell lines, we identified protein and phosphosites differentially expressed in the resistance context. Further data integration in a molecular network highlighted the differentially expressed pathways, in particular migration and invasion, RNA splicing, DNA damage repair response and transcription regulation. CONCLUSIONS: Overall, this study proposes a valuable resource toward the characterization of proteome and phosphoproteome of four widely used prostate cell lines and reveals candidates to be involved in prostate cancer progression for further experimental validation.
format Online
Article
Text
id pubmed-6824562
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-68245622019-11-12 Integrative proteomic and phosphoproteomic profiling of prostate cell lines Katsogiannou, Maria Boyer, Jean-Baptiste Valdeolivas, Alberto Remy, Elisabeth Calzone, Laurence Audebert, Stéphane Rocchi, Palma Camoin, Luc Baudot, Anaïs PLoS One Research Article BACKGROUND: Prostate cancer is a major public health issue, mainly because patients relapse after androgen deprivation therapy. Proteomic strategies, aiming to reflect the functional activity of cells, are nowadays among the leading approaches to tackle the challenges not only of better diagnosis, but also of unraveling mechanistic details related to disease etiology and progression. METHODS: We conducted here a large SILAC-based Mass Spectrometry experiment to map the proteomes and phosphoproteomes of four widely used prostate cell lines, namely PNT1A, LNCaP, DU145 and PC3, representative of different cancerous and hormonal status. RESULTS: We identified more than 3000 proteins and phosphosites, from which we quantified more than 1000 proteins and 500 phosphosites after stringent filtering. Extensive exploration of this proteomics and phosphoproteomics dataset allowed characterizing housekeeping as well as cell-line specific proteins, phosphosites and functional features of each cell line. In addition, by comparing the sensitive and resistant cell lines, we identified protein and phosphosites differentially expressed in the resistance context. Further data integration in a molecular network highlighted the differentially expressed pathways, in particular migration and invasion, RNA splicing, DNA damage repair response and transcription regulation. CONCLUSIONS: Overall, this study proposes a valuable resource toward the characterization of proteome and phosphoproteome of four widely used prostate cell lines and reveals candidates to be involved in prostate cancer progression for further experimental validation. Public Library of Science 2019-11-01 /pmc/articles/PMC6824562/ /pubmed/31675377 http://dx.doi.org/10.1371/journal.pone.0224148 Text en © 2019 Katsogiannou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Katsogiannou, Maria
Boyer, Jean-Baptiste
Valdeolivas, Alberto
Remy, Elisabeth
Calzone, Laurence
Audebert, Stéphane
Rocchi, Palma
Camoin, Luc
Baudot, Anaïs
Integrative proteomic and phosphoproteomic profiling of prostate cell lines
title Integrative proteomic and phosphoproteomic profiling of prostate cell lines
title_full Integrative proteomic and phosphoproteomic profiling of prostate cell lines
title_fullStr Integrative proteomic and phosphoproteomic profiling of prostate cell lines
title_full_unstemmed Integrative proteomic and phosphoproteomic profiling of prostate cell lines
title_short Integrative proteomic and phosphoproteomic profiling of prostate cell lines
title_sort integrative proteomic and phosphoproteomic profiling of prostate cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824562/
https://www.ncbi.nlm.nih.gov/pubmed/31675377
http://dx.doi.org/10.1371/journal.pone.0224148
work_keys_str_mv AT katsogiannoumaria integrativeproteomicandphosphoproteomicprofilingofprostatecelllines
AT boyerjeanbaptiste integrativeproteomicandphosphoproteomicprofilingofprostatecelllines
AT valdeolivasalberto integrativeproteomicandphosphoproteomicprofilingofprostatecelllines
AT remyelisabeth integrativeproteomicandphosphoproteomicprofilingofprostatecelllines
AT calzonelaurence integrativeproteomicandphosphoproteomicprofilingofprostatecelllines
AT audebertstephane integrativeproteomicandphosphoproteomicprofilingofprostatecelllines
AT rocchipalma integrativeproteomicandphosphoproteomicprofilingofprostatecelllines
AT camoinluc integrativeproteomicandphosphoproteomicprofilingofprostatecelllines
AT baudotanais integrativeproteomicandphosphoproteomicprofilingofprostatecelllines