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Low Expression of Occludin in the Melanoma Patient

BACKGROUND & OBJECTIVE: Malignant melanoma is the fatal cutaneous neoplasm which is curable by the early diagnosis. The expression of occludin protein which is an integral membrane protein is altered in an epithelial-to-mesenchymal transition. Although, recent studies provide sufficient evidence...

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Autores principales: Salehi, Pouri, Tafvizi, Farzaneh, Kamyab Hesari, Kambiz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Pathology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824771/
https://www.ncbi.nlm.nih.gov/pubmed/31754355
http://dx.doi.org/10.30699/ijp.2019.85213.1801
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author Salehi, Pouri
Tafvizi, Farzaneh
Kamyab Hesari, Kambiz
author_facet Salehi, Pouri
Tafvizi, Farzaneh
Kamyab Hesari, Kambiz
author_sort Salehi, Pouri
collection PubMed
description BACKGROUND & OBJECTIVE: Malignant melanoma is the fatal cutaneous neoplasm which is curable by the early diagnosis. The expression of occludin protein which is an integral membrane protein is altered in an epithelial-to-mesenchymal transition. Although, recent studies provide sufficient evidence supporting the functional importance of occludin in cancer, the prognostic significance of occludin expression levels in melanoma remains obscure. The aim of this study was to determine occludin expression level and its correlation with clinicopathological features of the patients with melanoma. METHODS: The occludin mRNA level was compared between paraffin-embedded tissues of 40 patients with melanoma and 10 subjects with normal skin. The quality and quantity of the RNA was determined and occludin expression level was measured using Real-time PCR and ∆∆CT computational technique. RESULTS: The occludin mRNA level reduced five-fold in the melanoma patients compared to the control group (P=0.000). No significant difference was observed between male and female cases (P=0.533). No significant correlation was observed between occludin mRNA level, mitotic count (P=0.252), and Breslow levels (P=0.171) CONCLUSION: We can conclude that down-regulation of occludin expression in the patients with melanoma is a hallmark of cancer progression and it might be used as a prognostic factor. No significant correlation was found between occludin gene expression and clinicopathological characteristics including Clark level, Breslow staging, mitotic count, age and gender (P<0.05).
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spelling pubmed-68247712019-11-21 Low Expression of Occludin in the Melanoma Patient Salehi, Pouri Tafvizi, Farzaneh Kamyab Hesari, Kambiz Iran J Pathol Original Article BACKGROUND & OBJECTIVE: Malignant melanoma is the fatal cutaneous neoplasm which is curable by the early diagnosis. The expression of occludin protein which is an integral membrane protein is altered in an epithelial-to-mesenchymal transition. Although, recent studies provide sufficient evidence supporting the functional importance of occludin in cancer, the prognostic significance of occludin expression levels in melanoma remains obscure. The aim of this study was to determine occludin expression level and its correlation with clinicopathological features of the patients with melanoma. METHODS: The occludin mRNA level was compared between paraffin-embedded tissues of 40 patients with melanoma and 10 subjects with normal skin. The quality and quantity of the RNA was determined and occludin expression level was measured using Real-time PCR and ∆∆CT computational technique. RESULTS: The occludin mRNA level reduced five-fold in the melanoma patients compared to the control group (P=0.000). No significant difference was observed between male and female cases (P=0.533). No significant correlation was observed between occludin mRNA level, mitotic count (P=0.252), and Breslow levels (P=0.171) CONCLUSION: We can conclude that down-regulation of occludin expression in the patients with melanoma is a hallmark of cancer progression and it might be used as a prognostic factor. No significant correlation was found between occludin gene expression and clinicopathological characteristics including Clark level, Breslow staging, mitotic count, age and gender (P<0.05). Iranian Society of Pathology 2019 2019-09-22 /pmc/articles/PMC6824771/ /pubmed/31754355 http://dx.doi.org/10.30699/ijp.2019.85213.1801 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Salehi, Pouri
Tafvizi, Farzaneh
Kamyab Hesari, Kambiz
Low Expression of Occludin in the Melanoma Patient
title Low Expression of Occludin in the Melanoma Patient
title_full Low Expression of Occludin in the Melanoma Patient
title_fullStr Low Expression of Occludin in the Melanoma Patient
title_full_unstemmed Low Expression of Occludin in the Melanoma Patient
title_short Low Expression of Occludin in the Melanoma Patient
title_sort low expression of occludin in the melanoma patient
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824771/
https://www.ncbi.nlm.nih.gov/pubmed/31754355
http://dx.doi.org/10.30699/ijp.2019.85213.1801
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