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The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue
Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective pla...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824906/ https://www.ncbi.nlm.nih.gov/pubmed/31527839 http://dx.doi.org/10.1038/s41592-019-0554-0 |
| _version_ | 1783464822075031552 |
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| author | Voigt, Fabian F. Kirschenbaum, Daniel Platonova, Evgenia Pagès, Stéphane Campbell, Robert A. A. Kastli, Rahel Schaettin, Martina Egolf, Ladan van der Bourg, Alexander Bethge, Philipp Haenraets, Karen Frézel, Noémie Topilko, Thomas Perin, Paola Hillier, Daniel Hildebrand, Sven Schueth, Anna Roebroeck, Alard Roska, Botond Stoeckli, Esther T. Pizzala, Roberto Renier, Nicolas Zeilhofer, Hanns Ulrich Karayannis, Theofanis Ziegler, Urs Batti, Laura Holtmaat, Anthony Lüscher, Christian Aguzzi, Adriano Helmchen, Fritjof |
| author_facet | Voigt, Fabian F. Kirschenbaum, Daniel Platonova, Evgenia Pagès, Stéphane Campbell, Robert A. A. Kastli, Rahel Schaettin, Martina Egolf, Ladan van der Bourg, Alexander Bethge, Philipp Haenraets, Karen Frézel, Noémie Topilko, Thomas Perin, Paola Hillier, Daniel Hildebrand, Sven Schueth, Anna Roebroeck, Alard Roska, Botond Stoeckli, Esther T. Pizzala, Roberto Renier, Nicolas Zeilhofer, Hanns Ulrich Karayannis, Theofanis Ziegler, Urs Batti, Laura Holtmaat, Anthony Lüscher, Christian Aguzzi, Adriano Helmchen, Fritjof |
| author_sort | Voigt, Fabian F. |
| collection | PubMed |
| description | Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy (mesoSPIM) initiative, an open-hardware project for building and operating a light sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample (www.mesospim.org). |
| format | Online Article Text |
| id | pubmed-6824906 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-68249062020-03-16 The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue Voigt, Fabian F. Kirschenbaum, Daniel Platonova, Evgenia Pagès, Stéphane Campbell, Robert A. A. Kastli, Rahel Schaettin, Martina Egolf, Ladan van der Bourg, Alexander Bethge, Philipp Haenraets, Karen Frézel, Noémie Topilko, Thomas Perin, Paola Hillier, Daniel Hildebrand, Sven Schueth, Anna Roebroeck, Alard Roska, Botond Stoeckli, Esther T. Pizzala, Roberto Renier, Nicolas Zeilhofer, Hanns Ulrich Karayannis, Theofanis Ziegler, Urs Batti, Laura Holtmaat, Anthony Lüscher, Christian Aguzzi, Adriano Helmchen, Fritjof Nat Methods Article Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy (mesoSPIM) initiative, an open-hardware project for building and operating a light sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample (www.mesospim.org). 2019-09-16 2019-11 /pmc/articles/PMC6824906/ /pubmed/31527839 http://dx.doi.org/10.1038/s41592-019-0554-0 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Voigt, Fabian F. Kirschenbaum, Daniel Platonova, Evgenia Pagès, Stéphane Campbell, Robert A. A. Kastli, Rahel Schaettin, Martina Egolf, Ladan van der Bourg, Alexander Bethge, Philipp Haenraets, Karen Frézel, Noémie Topilko, Thomas Perin, Paola Hillier, Daniel Hildebrand, Sven Schueth, Anna Roebroeck, Alard Roska, Botond Stoeckli, Esther T. Pizzala, Roberto Renier, Nicolas Zeilhofer, Hanns Ulrich Karayannis, Theofanis Ziegler, Urs Batti, Laura Holtmaat, Anthony Lüscher, Christian Aguzzi, Adriano Helmchen, Fritjof The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue |
| title | The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue |
| title_full | The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue |
| title_fullStr | The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue |
| title_full_unstemmed | The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue |
| title_short | The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue |
| title_sort | mesospim initiative – open-source light-sheet microscopes for imaging cleared tissue |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824906/ https://www.ncbi.nlm.nih.gov/pubmed/31527839 http://dx.doi.org/10.1038/s41592-019-0554-0 |
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