Cargando…

Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa

Pseudomonas aeruginosa, an opportunistic pathogen of humans, uses quorum sensing (QS) to regulate the production of extracellular products that can benefit all members of the population. P. aeruginosa can police QS-deficient cheaters by producing hydrogen cyanide, which is also QS regulated; however...

Descripción completa

Detalles Bibliográficos
Autores principales: Yan, Huicong, Asfahl, Kyle L., Li, Na, Sun, Feng, Xiao, Junwei, Shen, Dongsheng, Dandekar, Ajai A., Wang, Meizhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825135/
https://www.ncbi.nlm.nih.gov/pubmed/31676850
http://dx.doi.org/10.1038/s41467-019-13013-8
_version_ 1783464843701911552
author Yan, Huicong
Asfahl, Kyle L.
Li, Na
Sun, Feng
Xiao, Junwei
Shen, Dongsheng
Dandekar, Ajai A.
Wang, Meizhen
author_facet Yan, Huicong
Asfahl, Kyle L.
Li, Na
Sun, Feng
Xiao, Junwei
Shen, Dongsheng
Dandekar, Ajai A.
Wang, Meizhen
author_sort Yan, Huicong
collection PubMed
description Pseudomonas aeruginosa, an opportunistic pathogen of humans, uses quorum sensing (QS) to regulate the production of extracellular products that can benefit all members of the population. P. aeruginosa can police QS-deficient cheaters by producing hydrogen cyanide, which is also QS regulated; however, the mechanism by which cooperators selectively protect themselves from the toxicity of cyanide remained unresolved. Here, we show that a cyanide-insensitive terminal oxidase encoded by cioAB provides resistance to cyanide, but only in QS-proficient strains. QS-deficient cheaters do not activate cioAB transcription. QS-mediated regulation of cioAB expression depends on production of both cyanide by cooperators (which is QS regulated) and reactive oxygen species (ROS) from cheaters (which is not QS regulated). This type of regulatory system allows cooperating populations to respond, via ROS, to the presence of cheaters, and might allow them to defer the substantial metabolic cost of policing until cheaters are present in the population.
format Online
Article
Text
id pubmed-6825135
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-68251352019-11-04 Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa Yan, Huicong Asfahl, Kyle L. Li, Na Sun, Feng Xiao, Junwei Shen, Dongsheng Dandekar, Ajai A. Wang, Meizhen Nat Commun Article Pseudomonas aeruginosa, an opportunistic pathogen of humans, uses quorum sensing (QS) to regulate the production of extracellular products that can benefit all members of the population. P. aeruginosa can police QS-deficient cheaters by producing hydrogen cyanide, which is also QS regulated; however, the mechanism by which cooperators selectively protect themselves from the toxicity of cyanide remained unresolved. Here, we show that a cyanide-insensitive terminal oxidase encoded by cioAB provides resistance to cyanide, but only in QS-proficient strains. QS-deficient cheaters do not activate cioAB transcription. QS-mediated regulation of cioAB expression depends on production of both cyanide by cooperators (which is QS regulated) and reactive oxygen species (ROS) from cheaters (which is not QS regulated). This type of regulatory system allows cooperating populations to respond, via ROS, to the presence of cheaters, and might allow them to defer the substantial metabolic cost of policing until cheaters are present in the population. Nature Publishing Group UK 2019-11-01 /pmc/articles/PMC6825135/ /pubmed/31676850 http://dx.doi.org/10.1038/s41467-019-13013-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yan, Huicong
Asfahl, Kyle L.
Li, Na
Sun, Feng
Xiao, Junwei
Shen, Dongsheng
Dandekar, Ajai A.
Wang, Meizhen
Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa
title Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa
title_full Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa
title_fullStr Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa
title_full_unstemmed Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa
title_short Conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of Pseudomonas aeruginosa
title_sort conditional quorum-sensing induction of a cyanide-insensitive terminal oxidase stabilizes cooperating populations of pseudomonas aeruginosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825135/
https://www.ncbi.nlm.nih.gov/pubmed/31676850
http://dx.doi.org/10.1038/s41467-019-13013-8
work_keys_str_mv AT yanhuicong conditionalquorumsensinginductionofacyanideinsensitiveterminaloxidasestabilizescooperatingpopulationsofpseudomonasaeruginosa
AT asfahlkylel conditionalquorumsensinginductionofacyanideinsensitiveterminaloxidasestabilizescooperatingpopulationsofpseudomonasaeruginosa
AT lina conditionalquorumsensinginductionofacyanideinsensitiveterminaloxidasestabilizescooperatingpopulationsofpseudomonasaeruginosa
AT sunfeng conditionalquorumsensinginductionofacyanideinsensitiveterminaloxidasestabilizescooperatingpopulationsofpseudomonasaeruginosa
AT xiaojunwei conditionalquorumsensinginductionofacyanideinsensitiveterminaloxidasestabilizescooperatingpopulationsofpseudomonasaeruginosa
AT shendongsheng conditionalquorumsensinginductionofacyanideinsensitiveterminaloxidasestabilizescooperatingpopulationsofpseudomonasaeruginosa
AT dandekarajaia conditionalquorumsensinginductionofacyanideinsensitiveterminaloxidasestabilizescooperatingpopulationsofpseudomonasaeruginosa
AT wangmeizhen conditionalquorumsensinginductionofacyanideinsensitiveterminaloxidasestabilizescooperatingpopulationsofpseudomonasaeruginosa