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Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma

Canine cutaneous squamous cell carcinoma (cSCC) is the most common skin cancer in dogs, and, due to its low metastatic rate, local treatments, such as electrochemotherapy (ECT), promote disease control or even complete remission (CR). This study aimed to evaluate the gene and protein expression of B...

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Autores principales: Dos Anjos, Denner S., Bueno, Cynthia, Magalhães, Larissa F., Magalhães, Georgia M., Mattos-Junior, Ewaldo, Pinto, Marcela M. R., De Nardi, Andrigo B., Brunner, Carlos H. M., Leis-Filho, Antonio F., Calazans, Sabryna G., Fonseca-Alves, Carlos E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825193/
https://www.ncbi.nlm.nih.gov/pubmed/31676831
http://dx.doi.org/10.1038/s41598-019-52461-6
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author Dos Anjos, Denner S.
Bueno, Cynthia
Magalhães, Larissa F.
Magalhães, Georgia M.
Mattos-Junior, Ewaldo
Pinto, Marcela M. R.
De Nardi, Andrigo B.
Brunner, Carlos H. M.
Leis-Filho, Antonio F.
Calazans, Sabryna G.
Fonseca-Alves, Carlos E.
author_facet Dos Anjos, Denner S.
Bueno, Cynthia
Magalhães, Larissa F.
Magalhães, Georgia M.
Mattos-Junior, Ewaldo
Pinto, Marcela M. R.
De Nardi, Andrigo B.
Brunner, Carlos H. M.
Leis-Filho, Antonio F.
Calazans, Sabryna G.
Fonseca-Alves, Carlos E.
author_sort Dos Anjos, Denner S.
collection PubMed
description Canine cutaneous squamous cell carcinoma (cSCC) is the most common skin cancer in dogs, and, due to its low metastatic rate, local treatments, such as electrochemotherapy (ECT), promote disease control or even complete remission (CR). This study aimed to evaluate the gene and protein expression of Bcl-2 and Bcl-2 associated X protein (BAX), the proliferative index and clinical parameters in dogs with cSCC subjected to ECT. A prospective nonrandomized clinical study was performed using dogs with naturally occurring cSCC that was treated with ECT. Eighteen lesions from 11 dogs were selected. The tumor size at day 0 (D0) had no impact on survival or prognosis (P > 0.05). Tumor samples had a lower proliferative index after ECT (D21) than before ECT (P = 0.031). The survival of subjects with Ki67 values lower and higher than the Ki67 median value were not significantly different (P > 0.05). Regarding apoptotic markers, there were no significant differences in the gene and protein expression levels of BAX or Bcl-2 at D0 and D21 (P > 0.05) or in the overall survival of subjects with different levels of apoptotic markers. In conclusion, there was no change in BAX or Bcl-2 gene and protein expression in response to ECT at the time points evaluated, but ECT was able to reduce tumor volume and cellular proliferation in cSCC.
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spelling pubmed-68251932019-11-12 Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma Dos Anjos, Denner S. Bueno, Cynthia Magalhães, Larissa F. Magalhães, Georgia M. Mattos-Junior, Ewaldo Pinto, Marcela M. R. De Nardi, Andrigo B. Brunner, Carlos H. M. Leis-Filho, Antonio F. Calazans, Sabryna G. Fonseca-Alves, Carlos E. Sci Rep Article Canine cutaneous squamous cell carcinoma (cSCC) is the most common skin cancer in dogs, and, due to its low metastatic rate, local treatments, such as electrochemotherapy (ECT), promote disease control or even complete remission (CR). This study aimed to evaluate the gene and protein expression of Bcl-2 and Bcl-2 associated X protein (BAX), the proliferative index and clinical parameters in dogs with cSCC subjected to ECT. A prospective nonrandomized clinical study was performed using dogs with naturally occurring cSCC that was treated with ECT. Eighteen lesions from 11 dogs were selected. The tumor size at day 0 (D0) had no impact on survival or prognosis (P > 0.05). Tumor samples had a lower proliferative index after ECT (D21) than before ECT (P = 0.031). The survival of subjects with Ki67 values lower and higher than the Ki67 median value were not significantly different (P > 0.05). Regarding apoptotic markers, there were no significant differences in the gene and protein expression levels of BAX or Bcl-2 at D0 and D21 (P > 0.05) or in the overall survival of subjects with different levels of apoptotic markers. In conclusion, there was no change in BAX or Bcl-2 gene and protein expression in response to ECT at the time points evaluated, but ECT was able to reduce tumor volume and cellular proliferation in cSCC. Nature Publishing Group UK 2019-11-01 /pmc/articles/PMC6825193/ /pubmed/31676831 http://dx.doi.org/10.1038/s41598-019-52461-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dos Anjos, Denner S.
Bueno, Cynthia
Magalhães, Larissa F.
Magalhães, Georgia M.
Mattos-Junior, Ewaldo
Pinto, Marcela M. R.
De Nardi, Andrigo B.
Brunner, Carlos H. M.
Leis-Filho, Antonio F.
Calazans, Sabryna G.
Fonseca-Alves, Carlos E.
Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
title Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
title_full Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
title_fullStr Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
title_full_unstemmed Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
title_short Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
title_sort electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825193/
https://www.ncbi.nlm.nih.gov/pubmed/31676831
http://dx.doi.org/10.1038/s41598-019-52461-6
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