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Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
Canine cutaneous squamous cell carcinoma (cSCC) is the most common skin cancer in dogs, and, due to its low metastatic rate, local treatments, such as electrochemotherapy (ECT), promote disease control or even complete remission (CR). This study aimed to evaluate the gene and protein expression of B...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825193/ https://www.ncbi.nlm.nih.gov/pubmed/31676831 http://dx.doi.org/10.1038/s41598-019-52461-6 |
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author | Dos Anjos, Denner S. Bueno, Cynthia Magalhães, Larissa F. Magalhães, Georgia M. Mattos-Junior, Ewaldo Pinto, Marcela M. R. De Nardi, Andrigo B. Brunner, Carlos H. M. Leis-Filho, Antonio F. Calazans, Sabryna G. Fonseca-Alves, Carlos E. |
author_facet | Dos Anjos, Denner S. Bueno, Cynthia Magalhães, Larissa F. Magalhães, Georgia M. Mattos-Junior, Ewaldo Pinto, Marcela M. R. De Nardi, Andrigo B. Brunner, Carlos H. M. Leis-Filho, Antonio F. Calazans, Sabryna G. Fonseca-Alves, Carlos E. |
author_sort | Dos Anjos, Denner S. |
collection | PubMed |
description | Canine cutaneous squamous cell carcinoma (cSCC) is the most common skin cancer in dogs, and, due to its low metastatic rate, local treatments, such as electrochemotherapy (ECT), promote disease control or even complete remission (CR). This study aimed to evaluate the gene and protein expression of Bcl-2 and Bcl-2 associated X protein (BAX), the proliferative index and clinical parameters in dogs with cSCC subjected to ECT. A prospective nonrandomized clinical study was performed using dogs with naturally occurring cSCC that was treated with ECT. Eighteen lesions from 11 dogs were selected. The tumor size at day 0 (D0) had no impact on survival or prognosis (P > 0.05). Tumor samples had a lower proliferative index after ECT (D21) than before ECT (P = 0.031). The survival of subjects with Ki67 values lower and higher than the Ki67 median value were not significantly different (P > 0.05). Regarding apoptotic markers, there were no significant differences in the gene and protein expression levels of BAX or Bcl-2 at D0 and D21 (P > 0.05) or in the overall survival of subjects with different levels of apoptotic markers. In conclusion, there was no change in BAX or Bcl-2 gene and protein expression in response to ECT at the time points evaluated, but ECT was able to reduce tumor volume and cellular proliferation in cSCC. |
format | Online Article Text |
id | pubmed-6825193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68251932019-11-12 Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma Dos Anjos, Denner S. Bueno, Cynthia Magalhães, Larissa F. Magalhães, Georgia M. Mattos-Junior, Ewaldo Pinto, Marcela M. R. De Nardi, Andrigo B. Brunner, Carlos H. M. Leis-Filho, Antonio F. Calazans, Sabryna G. Fonseca-Alves, Carlos E. Sci Rep Article Canine cutaneous squamous cell carcinoma (cSCC) is the most common skin cancer in dogs, and, due to its low metastatic rate, local treatments, such as electrochemotherapy (ECT), promote disease control or even complete remission (CR). This study aimed to evaluate the gene and protein expression of Bcl-2 and Bcl-2 associated X protein (BAX), the proliferative index and clinical parameters in dogs with cSCC subjected to ECT. A prospective nonrandomized clinical study was performed using dogs with naturally occurring cSCC that was treated with ECT. Eighteen lesions from 11 dogs were selected. The tumor size at day 0 (D0) had no impact on survival or prognosis (P > 0.05). Tumor samples had a lower proliferative index after ECT (D21) than before ECT (P = 0.031). The survival of subjects with Ki67 values lower and higher than the Ki67 median value were not significantly different (P > 0.05). Regarding apoptotic markers, there were no significant differences in the gene and protein expression levels of BAX or Bcl-2 at D0 and D21 (P > 0.05) or in the overall survival of subjects with different levels of apoptotic markers. In conclusion, there was no change in BAX or Bcl-2 gene and protein expression in response to ECT at the time points evaluated, but ECT was able to reduce tumor volume and cellular proliferation in cSCC. Nature Publishing Group UK 2019-11-01 /pmc/articles/PMC6825193/ /pubmed/31676831 http://dx.doi.org/10.1038/s41598-019-52461-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Dos Anjos, Denner S. Bueno, Cynthia Magalhães, Larissa F. Magalhães, Georgia M. Mattos-Junior, Ewaldo Pinto, Marcela M. R. De Nardi, Andrigo B. Brunner, Carlos H. M. Leis-Filho, Antonio F. Calazans, Sabryna G. Fonseca-Alves, Carlos E. Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma |
title | Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma |
title_full | Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma |
title_fullStr | Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma |
title_full_unstemmed | Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma |
title_short | Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma |
title_sort | electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825193/ https://www.ncbi.nlm.nih.gov/pubmed/31676831 http://dx.doi.org/10.1038/s41598-019-52461-6 |
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