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Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals
It is highly intriguing how bacterial pathogens can quickly shut down energy-costly infection machinery once successful infection is established. This study depicts that mutation of repressor SghR increases the expression of hydrolase SghA in Agrobacterium tumefaciens, which releases plant defense s...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825286/ https://www.ncbi.nlm.nih.gov/pubmed/31604827 http://dx.doi.org/10.1073/pnas.1903695116 |
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author | Wang, Chao Ye, Fuzhou Chang, Changqing Liu, Xiaoling Wang, Jianhe Wang, Jinpei Yan, Xin-Fu Fu, Qinqin Zhou, Jianuan Chen, Shaohua Gao, Yong-Gui Zhang, Lian-Hui |
author_facet | Wang, Chao Ye, Fuzhou Chang, Changqing Liu, Xiaoling Wang, Jianhe Wang, Jinpei Yan, Xin-Fu Fu, Qinqin Zhou, Jianuan Chen, Shaohua Gao, Yong-Gui Zhang, Lian-Hui |
author_sort | Wang, Chao |
collection | PubMed |
description | It is highly intriguing how bacterial pathogens can quickly shut down energy-costly infection machinery once successful infection is established. This study depicts that mutation of repressor SghR increases the expression of hydrolase SghA in Agrobacterium tumefaciens, which releases plant defense signal salicylic acid (SA) from its storage form SA β-glucoside (SAG). Addition of SA substantially reduces gene expression of bacterial virulence. Bacterial vir genes and sghA are differentially transcribed at early and later infection stages, respectively. Plant metabolite sucrose is a signal ligand that inactivates SghR and consequently induces sghA expression. Disruption of sghA leads to increased vir expression in planta and enhances tumor formation whereas mutation of sghR decreases vir expression and tumor formation. These results depict a remarkable mechanism by which A. tumefaciens taps on the reserved pool of plant signal SA to reprogram its virulence upon establishment of infection. |
format | Online Article Text |
id | pubmed-6825286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-68252862019-11-06 Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals Wang, Chao Ye, Fuzhou Chang, Changqing Liu, Xiaoling Wang, Jianhe Wang, Jinpei Yan, Xin-Fu Fu, Qinqin Zhou, Jianuan Chen, Shaohua Gao, Yong-Gui Zhang, Lian-Hui Proc Natl Acad Sci U S A PNAS Plus It is highly intriguing how bacterial pathogens can quickly shut down energy-costly infection machinery once successful infection is established. This study depicts that mutation of repressor SghR increases the expression of hydrolase SghA in Agrobacterium tumefaciens, which releases plant defense signal salicylic acid (SA) from its storage form SA β-glucoside (SAG). Addition of SA substantially reduces gene expression of bacterial virulence. Bacterial vir genes and sghA are differentially transcribed at early and later infection stages, respectively. Plant metabolite sucrose is a signal ligand that inactivates SghR and consequently induces sghA expression. Disruption of sghA leads to increased vir expression in planta and enhances tumor formation whereas mutation of sghR decreases vir expression and tumor formation. These results depict a remarkable mechanism by which A. tumefaciens taps on the reserved pool of plant signal SA to reprogram its virulence upon establishment of infection. National Academy of Sciences 2019-10-29 2019-10-11 /pmc/articles/PMC6825286/ /pubmed/31604827 http://dx.doi.org/10.1073/pnas.1903695116 Text en Copyright © 2019 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | PNAS Plus Wang, Chao Ye, Fuzhou Chang, Changqing Liu, Xiaoling Wang, Jianhe Wang, Jinpei Yan, Xin-Fu Fu, Qinqin Zhou, Jianuan Chen, Shaohua Gao, Yong-Gui Zhang, Lian-Hui Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals |
title | Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals |
title_full | Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals |
title_fullStr | Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals |
title_full_unstemmed | Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals |
title_short | Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals |
title_sort | agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals |
topic | PNAS Plus |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825286/ https://www.ncbi.nlm.nih.gov/pubmed/31604827 http://dx.doi.org/10.1073/pnas.1903695116 |
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