Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis

BACKGROUND: Osteosarcoma (OS) is a very aggressive cancer. Nevertheless, how circular RNA (circRNA) contributes to OS progression remains unclear. Here, we aimed to research the functions of circMMP9 in OS progression. METHODS: Gene expression was determined via qRT-PCR. siRNA was used to knock down...

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Detalles Bibliográficos
Autores principales: Pan, Guangjie, Hu, Ting, Chen, Xuewu, Zhang, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825504/
https://www.ncbi.nlm.nih.gov/pubmed/31754311
http://dx.doi.org/10.2147/CMAR.S226264
Descripción
Sumario:BACKGROUND: Osteosarcoma (OS) is a very aggressive cancer. Nevertheless, how circular RNA (circRNA) contributes to OS progression remains unclear. Here, we aimed to research the functions of circMMP9 in OS progression. METHODS: Gene expression was determined via qRT-PCR. siRNA was used to knock down circMMP9. Proliferation was analyzed using CCK8 and colony formation assays. Migration and invasion were measured using Transwell assay. RESULTS: circMMP9 was overexpressed in cancer tissues. Overexpressed circMMP9 was correlated with advanced tumor stage and predicted poor prognosis. circMMP9 knockdown exhibited a tumor-suppressive phenotype via suppressing proliferation, migration and invasion. Besides, decreased circMMP9 level promoted OS cellular apoptosis. Mechanistically, circMMP9 was shown to be located in the cytoplasm and sponge miR-1265. Furthermore, miR-1265 directly targeted CHI3L1. CHI3L1 levels were modulated by circMMP9/miR-1265 axis. Rescue experiments indicated that circMMP9 contributes to OS development through the miR-1265/CHI3L1 pathway. CONCLUSION: Our findings provide a novel insight about how circRNA regulates OS progression.

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