Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis

BACKGROUND: Osteosarcoma (OS) is a very aggressive cancer. Nevertheless, how circular RNA (circRNA) contributes to OS progression remains unclear. Here, we aimed to research the functions of circMMP9 in OS progression. METHODS: Gene expression was determined via qRT-PCR. siRNA was used to knock down...

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Autores principales: Pan, Guangjie, Hu, Ting, Chen, Xuewu, Zhang, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825504/
https://www.ncbi.nlm.nih.gov/pubmed/31754311
http://dx.doi.org/10.2147/CMAR.S226264
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author Pan, Guangjie
Hu, Ting
Chen, Xuewu
Zhang, Chao
author_facet Pan, Guangjie
Hu, Ting
Chen, Xuewu
Zhang, Chao
author_sort Pan, Guangjie
collection PubMed
description BACKGROUND: Osteosarcoma (OS) is a very aggressive cancer. Nevertheless, how circular RNA (circRNA) contributes to OS progression remains unclear. Here, we aimed to research the functions of circMMP9 in OS progression. METHODS: Gene expression was determined via qRT-PCR. siRNA was used to knock down circMMP9. Proliferation was analyzed using CCK8 and colony formation assays. Migration and invasion were measured using Transwell assay. RESULTS: circMMP9 was overexpressed in cancer tissues. Overexpressed circMMP9 was correlated with advanced tumor stage and predicted poor prognosis. circMMP9 knockdown exhibited a tumor-suppressive phenotype via suppressing proliferation, migration and invasion. Besides, decreased circMMP9 level promoted OS cellular apoptosis. Mechanistically, circMMP9 was shown to be located in the cytoplasm and sponge miR-1265. Furthermore, miR-1265 directly targeted CHI3L1. CHI3L1 levels were modulated by circMMP9/miR-1265 axis. Rescue experiments indicated that circMMP9 contributes to OS development through the miR-1265/CHI3L1 pathway. CONCLUSION: Our findings provide a novel insight about how circRNA regulates OS progression.
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spelling pubmed-68255042019-11-21 Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis Pan, Guangjie Hu, Ting Chen, Xuewu Zhang, Chao Cancer Manag Res Original Research BACKGROUND: Osteosarcoma (OS) is a very aggressive cancer. Nevertheless, how circular RNA (circRNA) contributes to OS progression remains unclear. Here, we aimed to research the functions of circMMP9 in OS progression. METHODS: Gene expression was determined via qRT-PCR. siRNA was used to knock down circMMP9. Proliferation was analyzed using CCK8 and colony formation assays. Migration and invasion were measured using Transwell assay. RESULTS: circMMP9 was overexpressed in cancer tissues. Overexpressed circMMP9 was correlated with advanced tumor stage and predicted poor prognosis. circMMP9 knockdown exhibited a tumor-suppressive phenotype via suppressing proliferation, migration and invasion. Besides, decreased circMMP9 level promoted OS cellular apoptosis. Mechanistically, circMMP9 was shown to be located in the cytoplasm and sponge miR-1265. Furthermore, miR-1265 directly targeted CHI3L1. CHI3L1 levels were modulated by circMMP9/miR-1265 axis. Rescue experiments indicated that circMMP9 contributes to OS development through the miR-1265/CHI3L1 pathway. CONCLUSION: Our findings provide a novel insight about how circRNA regulates OS progression. Dove 2019-10-29 /pmc/articles/PMC6825504/ /pubmed/31754311 http://dx.doi.org/10.2147/CMAR.S226264 Text en © 2019 Pan et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Pan, Guangjie
Hu, Ting
Chen, Xuewu
Zhang, Chao
Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis
title Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis
title_full Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis
title_fullStr Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis
title_full_unstemmed Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis
title_short Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis
title_sort upregulation of circmmp9 promotes osteosarcoma progression via targeting mir-1265/chi3l1 axis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825504/
https://www.ncbi.nlm.nih.gov/pubmed/31754311
http://dx.doi.org/10.2147/CMAR.S226264
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AT chenxuewu upregulationofcircmmp9promotesosteosarcomaprogressionviatargetingmir1265chi3l1axis
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