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Overexpression of MdIAA9 confers high tolerance to osmotic stress in transgenic tobacco

Auxin is a plant hormone that takes part in a series of developmental and physiological processes. There are three major gene families that play a role in the early response of auxin and auxin/indole-3-acetic acid (Aux/IAA) is one of these. Although the genomic organization and function of Aux/IAA g...

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Autores principales: Huang, Dong, Wang, Qian, Duan, Dingyue, Dong, Qinglong, Zhao, Shuang, Zhang, Maoxue, Jing, Guangquan, Liu, Changhai, van Nocker, Steve, Ma, Fengwang, Li, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825743/
https://www.ncbi.nlm.nih.gov/pubmed/31687272
http://dx.doi.org/10.7717/peerj.7935
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author Huang, Dong
Wang, Qian
Duan, Dingyue
Dong, Qinglong
Zhao, Shuang
Zhang, Maoxue
Jing, Guangquan
Liu, Changhai
van Nocker, Steve
Ma, Fengwang
Li, Chao
author_facet Huang, Dong
Wang, Qian
Duan, Dingyue
Dong, Qinglong
Zhao, Shuang
Zhang, Maoxue
Jing, Guangquan
Liu, Changhai
van Nocker, Steve
Ma, Fengwang
Li, Chao
author_sort Huang, Dong
collection PubMed
description Auxin is a plant hormone that takes part in a series of developmental and physiological processes. There are three major gene families that play a role in the early response of auxin and auxin/indole-3-acetic acid (Aux/IAA) is one of these. Although the genomic organization and function of Aux/IAA genes have been recognized in reference plants there have only been a few focused studies conducted with non-model crop plants, especially in the woody perennial species. We conducted a genomic census and expression analysis of Aux/IAA genes in the cultivated apple (Malus × domestica Borkh.). The Aux/IAA gene family of the apple genome was identified and analyzed in this study. Phylogenetic analysis showed that MdIAAs could be categorized into nine subfamilies and that these MdIAA proteins contained four whole or partially conserved domains of the MdIAA family. The spatio-specific expression profiles showed that most of the MdIAAs were preferentially expressed in specific tissues. Some of these genes were significantly induced by treatments with one or more abiotic stresses. The overexpression of MdIAA9 in tobacco (Nicotiana tabacum L.) plants significantly increased their tolerance to osmotic stresses. Our cumulative data supports the interactions between abiotic stresses and plant hormones and provides a theoretical basis for the mechanism of Aux/IAA and drought resistance in apples.
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spelling pubmed-68257432019-11-04 Overexpression of MdIAA9 confers high tolerance to osmotic stress in transgenic tobacco Huang, Dong Wang, Qian Duan, Dingyue Dong, Qinglong Zhao, Shuang Zhang, Maoxue Jing, Guangquan Liu, Changhai van Nocker, Steve Ma, Fengwang Li, Chao PeerJ Ecology Auxin is a plant hormone that takes part in a series of developmental and physiological processes. There are three major gene families that play a role in the early response of auxin and auxin/indole-3-acetic acid (Aux/IAA) is one of these. Although the genomic organization and function of Aux/IAA genes have been recognized in reference plants there have only been a few focused studies conducted with non-model crop plants, especially in the woody perennial species. We conducted a genomic census and expression analysis of Aux/IAA genes in the cultivated apple (Malus × domestica Borkh.). The Aux/IAA gene family of the apple genome was identified and analyzed in this study. Phylogenetic analysis showed that MdIAAs could be categorized into nine subfamilies and that these MdIAA proteins contained four whole or partially conserved domains of the MdIAA family. The spatio-specific expression profiles showed that most of the MdIAAs were preferentially expressed in specific tissues. Some of these genes were significantly induced by treatments with one or more abiotic stresses. The overexpression of MdIAA9 in tobacco (Nicotiana tabacum L.) plants significantly increased their tolerance to osmotic stresses. Our cumulative data supports the interactions between abiotic stresses and plant hormones and provides a theoretical basis for the mechanism of Aux/IAA and drought resistance in apples. PeerJ Inc. 2019-10-31 /pmc/articles/PMC6825743/ /pubmed/31687272 http://dx.doi.org/10.7717/peerj.7935 Text en © 2019 Huang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Ecology
Huang, Dong
Wang, Qian
Duan, Dingyue
Dong, Qinglong
Zhao, Shuang
Zhang, Maoxue
Jing, Guangquan
Liu, Changhai
van Nocker, Steve
Ma, Fengwang
Li, Chao
Overexpression of MdIAA9 confers high tolerance to osmotic stress in transgenic tobacco
title Overexpression of MdIAA9 confers high tolerance to osmotic stress in transgenic tobacco
title_full Overexpression of MdIAA9 confers high tolerance to osmotic stress in transgenic tobacco
title_fullStr Overexpression of MdIAA9 confers high tolerance to osmotic stress in transgenic tobacco
title_full_unstemmed Overexpression of MdIAA9 confers high tolerance to osmotic stress in transgenic tobacco
title_short Overexpression of MdIAA9 confers high tolerance to osmotic stress in transgenic tobacco
title_sort overexpression of mdiaa9 confers high tolerance to osmotic stress in transgenic tobacco
topic Ecology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825743/
https://www.ncbi.nlm.nih.gov/pubmed/31687272
http://dx.doi.org/10.7717/peerj.7935
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