Cargando…
Intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis
BACKGROUND: Ulcerative colitis (UC) is a lifelong inflammatory bowel disease characterized by periods of intense colonic inflammation leading to debilitating symptoms. Delivery methods of current UC treatments are suboptimal and associated with side effects. Silica particles are a potential alternat...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hellenic Society of Gastroenterology
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826066/ https://www.ncbi.nlm.nih.gov/pubmed/31700235 http://dx.doi.org/10.20524/aog.2019.0411 |
_version_ | 1783465005096632320 |
---|---|
author | Tam, Shu Yie Janine Coller, Janet K. Wignall, Anthony Gibson, Rachel J. Khatri, Aparajita Barbé, Chris Bowen, Joanne M. |
author_facet | Tam, Shu Yie Janine Coller, Janet K. Wignall, Anthony Gibson, Rachel J. Khatri, Aparajita Barbé, Chris Bowen, Joanne M. |
author_sort | Tam, Shu Yie Janine |
collection | PubMed |
description | BACKGROUND: Ulcerative colitis (UC) is a lifelong inflammatory bowel disease characterized by periods of intense colonic inflammation leading to debilitating symptoms. Delivery methods of current UC treatments are suboptimal and associated with side effects. Silica particles are a potential alternative delivery method for UC therapeutics, given their promising drug-loading and safety profiles. However, it is unknown whether silica particles preferably accumulate at sites of colonic inflammation. This study aimed to correlate silica particle accumulation with colonic inflammation in a rat UC model. METHODS: Albino Wistar rats received 4.5% dextran sulfate sodium (DSS) in drinking water (n=6) for 7 days to induce UC. Control rats (n=6) received drinking water only. UC activity was assessed daily using disease activity index. All rats were orally gavaged with silica particles labeled with Alexa-633 tags on day 9, followed by imaging at 3, 6, and 24 h. Silica particle distribution and accumulation were examined using biophotonic imaging, confocal microscopy and fluorescent spectrophotometry. Rats were killed on day 10, with jejunum, ileum and colon collected for histopathological scoring and quantification of fluorescence. RESULTS: Rats treated with DSS had significantly higher UC disease activity (P=0.033) and colonic histopathological scores (P=0.0087) compared to controls. No statistically significant between-group differences in silica particle accumulation were seen on live imaging or tissue analysis. CONCLUSIONS: No correlation was seen between silica particle accumulation and colonic inflammation. However to draw clear conclusions, further research is required to establish the potential of silica particles as a UC-targeted delivery method. |
format | Online Article Text |
id | pubmed-6826066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hellenic Society of Gastroenterology |
record_format | MEDLINE/PubMed |
spelling | pubmed-68260662019-11-07 Intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis Tam, Shu Yie Janine Coller, Janet K. Wignall, Anthony Gibson, Rachel J. Khatri, Aparajita Barbé, Chris Bowen, Joanne M. Ann Gastroenterol Original Article BACKGROUND: Ulcerative colitis (UC) is a lifelong inflammatory bowel disease characterized by periods of intense colonic inflammation leading to debilitating symptoms. Delivery methods of current UC treatments are suboptimal and associated with side effects. Silica particles are a potential alternative delivery method for UC therapeutics, given their promising drug-loading and safety profiles. However, it is unknown whether silica particles preferably accumulate at sites of colonic inflammation. This study aimed to correlate silica particle accumulation with colonic inflammation in a rat UC model. METHODS: Albino Wistar rats received 4.5% dextran sulfate sodium (DSS) in drinking water (n=6) for 7 days to induce UC. Control rats (n=6) received drinking water only. UC activity was assessed daily using disease activity index. All rats were orally gavaged with silica particles labeled with Alexa-633 tags on day 9, followed by imaging at 3, 6, and 24 h. Silica particle distribution and accumulation were examined using biophotonic imaging, confocal microscopy and fluorescent spectrophotometry. Rats were killed on day 10, with jejunum, ileum and colon collected for histopathological scoring and quantification of fluorescence. RESULTS: Rats treated with DSS had significantly higher UC disease activity (P=0.033) and colonic histopathological scores (P=0.0087) compared to controls. No statistically significant between-group differences in silica particle accumulation were seen on live imaging or tissue analysis. CONCLUSIONS: No correlation was seen between silica particle accumulation and colonic inflammation. However to draw clear conclusions, further research is required to establish the potential of silica particles as a UC-targeted delivery method. Hellenic Society of Gastroenterology 2019 2019-08-03 /pmc/articles/PMC6826066/ /pubmed/31700235 http://dx.doi.org/10.20524/aog.2019.0411 Text en Copyright: © Hellenic Society of Gastroenterology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Tam, Shu Yie Janine Coller, Janet K. Wignall, Anthony Gibson, Rachel J. Khatri, Aparajita Barbé, Chris Bowen, Joanne M. Intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis |
title | Intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis |
title_full | Intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis |
title_fullStr | Intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis |
title_full_unstemmed | Intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis |
title_short | Intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis |
title_sort | intestinal accumulation of silica particles in a rat model of dextran sulfate sodium-induced colitis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826066/ https://www.ncbi.nlm.nih.gov/pubmed/31700235 http://dx.doi.org/10.20524/aog.2019.0411 |
work_keys_str_mv | AT tamshuyiejanine intestinalaccumulationofsilicaparticlesinaratmodelofdextransulfatesodiuminducedcolitis AT collerjanetk intestinalaccumulationofsilicaparticlesinaratmodelofdextransulfatesodiuminducedcolitis AT wignallanthony intestinalaccumulationofsilicaparticlesinaratmodelofdextransulfatesodiuminducedcolitis AT gibsonrachelj intestinalaccumulationofsilicaparticlesinaratmodelofdextransulfatesodiuminducedcolitis AT khatriaparajita intestinalaccumulationofsilicaparticlesinaratmodelofdextransulfatesodiuminducedcolitis AT barbechris intestinalaccumulationofsilicaparticlesinaratmodelofdextransulfatesodiuminducedcolitis AT bowenjoannem intestinalaccumulationofsilicaparticlesinaratmodelofdextransulfatesodiuminducedcolitis |