Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan

Here we report sequence and phylogenetic analysis of two new isolates of Leishmania RNA virus 2 (LRV2) found in Leishmania major isolated from human patients with cutaneous leishmaniasis in south Uzbekistan. These new virus-infected flagellates were isolated in the same region of Uzbekistan and the...

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Autores principales: Kleschenko, Yuliya, Grybchuk, Danyil, Matveeva, Nadezhda S., Macedo, Diego H., Ponirovsky, Evgeny N., Lukashev, Alexander N., Yurchenko, Vyacheslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826456/
https://www.ncbi.nlm.nih.gov/pubmed/31640177
http://dx.doi.org/10.3390/genes10100830
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author Kleschenko, Yuliya
Grybchuk, Danyil
Matveeva, Nadezhda S.
Macedo, Diego H.
Ponirovsky, Evgeny N.
Lukashev, Alexander N.
Yurchenko, Vyacheslav
author_facet Kleschenko, Yuliya
Grybchuk, Danyil
Matveeva, Nadezhda S.
Macedo, Diego H.
Ponirovsky, Evgeny N.
Lukashev, Alexander N.
Yurchenko, Vyacheslav
author_sort Kleschenko, Yuliya
collection PubMed
description Here we report sequence and phylogenetic analysis of two new isolates of Leishmania RNA virus 2 (LRV2) found in Leishmania major isolated from human patients with cutaneous leishmaniasis in south Uzbekistan. These new virus-infected flagellates were isolated in the same region of Uzbekistan and the viral sequences differed by only nineteen SNPs, all except one being silent mutations. Therefore, we concluded that they belong to a single LRV2 species. New viruses are closely related to the LRV2-Lmj-ASKH documented in Turkmenistan in 1995, which is congruent with their shared host (L. major) and common geographical origin.
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spelling pubmed-68264562019-11-18 Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan Kleschenko, Yuliya Grybchuk, Danyil Matveeva, Nadezhda S. Macedo, Diego H. Ponirovsky, Evgeny N. Lukashev, Alexander N. Yurchenko, Vyacheslav Genes (Basel) Article Here we report sequence and phylogenetic analysis of two new isolates of Leishmania RNA virus 2 (LRV2) found in Leishmania major isolated from human patients with cutaneous leishmaniasis in south Uzbekistan. These new virus-infected flagellates were isolated in the same region of Uzbekistan and the viral sequences differed by only nineteen SNPs, all except one being silent mutations. Therefore, we concluded that they belong to a single LRV2 species. New viruses are closely related to the LRV2-Lmj-ASKH documented in Turkmenistan in 1995, which is congruent with their shared host (L. major) and common geographical origin. MDPI 2019-10-21 /pmc/articles/PMC6826456/ /pubmed/31640177 http://dx.doi.org/10.3390/genes10100830 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kleschenko, Yuliya
Grybchuk, Danyil
Matveeva, Nadezhda S.
Macedo, Diego H.
Ponirovsky, Evgeny N.
Lukashev, Alexander N.
Yurchenko, Vyacheslav
Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan
title Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan
title_full Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan
title_fullStr Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan
title_full_unstemmed Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan
title_short Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan
title_sort molecular characterization of leishmania rna virus 2 in leishmania major from uzbekistan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826456/
https://www.ncbi.nlm.nih.gov/pubmed/31640177
http://dx.doi.org/10.3390/genes10100830
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