Cargando…
Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats
Reports from previous studies now provide evidence that dyslipidaemia and oxidative stress play crucial roles in the pathogenesis and progression of diabetes and its related complications. This research is aimed to investigate the potential effects of protein isolate from Parkia biglobosa seeds (PBP...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826478/ https://www.ncbi.nlm.nih.gov/pubmed/31614841 http://dx.doi.org/10.3390/antiox8100481 |
_version_ | 1783465096905752576 |
---|---|
author | Ogunyinka, Bolajoko Idiat Oyinloye, Babatunji Emmanuel Osunsanmi, Foluso Oluwagbemiga Kolanisi, Unathi Opoku, Andrew Rowland Kappo, Abidemi Paul |
author_facet | Ogunyinka, Bolajoko Idiat Oyinloye, Babatunji Emmanuel Osunsanmi, Foluso Oluwagbemiga Kolanisi, Unathi Opoku, Andrew Rowland Kappo, Abidemi Paul |
author_sort | Ogunyinka, Bolajoko Idiat |
collection | PubMed |
description | Reports from previous studies now provide evidence that dyslipidaemia and oxidative stress play crucial roles in the pathogenesis and progression of diabetes and its related complications. This research is aimed to investigate the potential effects of protein isolate from Parkia biglobosa seeds (PBPI) in streptozotocin (STZ)-induced diabetic rats by measuring blood glucose levels, changes in lipid metabolism and biomarkers of oxidative stress. Diabetic rats were treated orally with graded doses of PBPI, 200 mg/kg bw and 400 mg/kg bw, and 5 U/kg, intraperitoneal (i.p.) of insulin once daily for 28 days with the fasting blood glucose (FBG) monitored weekly. The effect of PBPI on the serum lipid profile was measured while the extent of lipid peroxidation (LPO), as well as antioxidant parameters (superoxide dismutase; SOD, catalase; CAT, glutathione-S-transferase; GST and total glutathione; total GSH), was determined in the cardiac homogenates of diabetic rats. At the tested doses, treatment with PBPI was significantly effective in lowering FBG, serum triglyceride, cholesterol, low-density lipoprotein cholesterol (LDL-c) and very low-density lipoprotein cholesterol (VLDL-c), while concurrently increasing high-density lipoprotein cholesterol (HDL-c). PBPI also significantly decreased the elevations witnessed in LPO levels and restored the biomarkers of oxidative stress in the cardiac homogenate of experimental rats. The results from this study demonstrate that PBPI could improve dyslipidaemia and cardiac oxidative stress in the experimental diabetic animal model possibly by reducing and effectively scavenging reactive oxygen species (ROS) production as well as by increasing antioxidant capacity in combating oxidative stress. Therefore, it can be concluded that PBPI could be explored in the development of a potent cardioprotective supplement or adjuvant therapy towards the management of diabetes and its related complications. |
format | Online Article Text |
id | pubmed-6826478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68264782019-11-18 Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats Ogunyinka, Bolajoko Idiat Oyinloye, Babatunji Emmanuel Osunsanmi, Foluso Oluwagbemiga Kolanisi, Unathi Opoku, Andrew Rowland Kappo, Abidemi Paul Antioxidants (Basel) Article Reports from previous studies now provide evidence that dyslipidaemia and oxidative stress play crucial roles in the pathogenesis and progression of diabetes and its related complications. This research is aimed to investigate the potential effects of protein isolate from Parkia biglobosa seeds (PBPI) in streptozotocin (STZ)-induced diabetic rats by measuring blood glucose levels, changes in lipid metabolism and biomarkers of oxidative stress. Diabetic rats were treated orally with graded doses of PBPI, 200 mg/kg bw and 400 mg/kg bw, and 5 U/kg, intraperitoneal (i.p.) of insulin once daily for 28 days with the fasting blood glucose (FBG) monitored weekly. The effect of PBPI on the serum lipid profile was measured while the extent of lipid peroxidation (LPO), as well as antioxidant parameters (superoxide dismutase; SOD, catalase; CAT, glutathione-S-transferase; GST and total glutathione; total GSH), was determined in the cardiac homogenates of diabetic rats. At the tested doses, treatment with PBPI was significantly effective in lowering FBG, serum triglyceride, cholesterol, low-density lipoprotein cholesterol (LDL-c) and very low-density lipoprotein cholesterol (VLDL-c), while concurrently increasing high-density lipoprotein cholesterol (HDL-c). PBPI also significantly decreased the elevations witnessed in LPO levels and restored the biomarkers of oxidative stress in the cardiac homogenate of experimental rats. The results from this study demonstrate that PBPI could improve dyslipidaemia and cardiac oxidative stress in the experimental diabetic animal model possibly by reducing and effectively scavenging reactive oxygen species (ROS) production as well as by increasing antioxidant capacity in combating oxidative stress. Therefore, it can be concluded that PBPI could be explored in the development of a potent cardioprotective supplement or adjuvant therapy towards the management of diabetes and its related complications. MDPI 2019-10-12 /pmc/articles/PMC6826478/ /pubmed/31614841 http://dx.doi.org/10.3390/antiox8100481 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ogunyinka, Bolajoko Idiat Oyinloye, Babatunji Emmanuel Osunsanmi, Foluso Oluwagbemiga Kolanisi, Unathi Opoku, Andrew Rowland Kappo, Abidemi Paul Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats |
title | Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats |
title_full | Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats |
title_fullStr | Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats |
title_full_unstemmed | Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats |
title_short | Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats |
title_sort | protein isolate from parkia biglobosa seeds improves dyslipidaemia and cardiac oxidative stress in streptozotocin-induced diabetic rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826478/ https://www.ncbi.nlm.nih.gov/pubmed/31614841 http://dx.doi.org/10.3390/antiox8100481 |
work_keys_str_mv | AT ogunyinkabolajokoidiat proteinisolatefromparkiabiglobosaseedsimprovesdyslipidaemiaandcardiacoxidativestressinstreptozotocininduceddiabeticrats AT oyinloyebabatunjiemmanuel proteinisolatefromparkiabiglobosaseedsimprovesdyslipidaemiaandcardiacoxidativestressinstreptozotocininduceddiabeticrats AT osunsanmifolusooluwagbemiga proteinisolatefromparkiabiglobosaseedsimprovesdyslipidaemiaandcardiacoxidativestressinstreptozotocininduceddiabeticrats AT kolanisiunathi proteinisolatefromparkiabiglobosaseedsimprovesdyslipidaemiaandcardiacoxidativestressinstreptozotocininduceddiabeticrats AT opokuandrewrowland proteinisolatefromparkiabiglobosaseedsimprovesdyslipidaemiaandcardiacoxidativestressinstreptozotocininduceddiabeticrats AT kappoabidemipaul proteinisolatefromparkiabiglobosaseedsimprovesdyslipidaemiaandcardiacoxidativestressinstreptozotocininduceddiabeticrats |