Effects of Dietary Threonine Levels on Intestinal Immunity and Antioxidant Capacity Based on Cecal Metabolites and Transcription Sequencing of Broiler

SIMPLE SUMMARY: Threonine (Thr), an indispensable amino acid for animals and the third limiting amino acid of broilers, plays a vital role in the synthesis of gut mucosal proteins, which also has better effects on growth performance, biochemical indexes, antioxidant function, and gut morphology, as well as acting as a nutrient immunomodulator that affects the intestinal barrier function of broilers. However, it is not clear how it works in depth. The objective of the current study was to investigate the mechanism of effects of different dietary threonine levels on the antioxidant and immune capacity of broilers. Our findings suggest that a Thr level of 125% NRC (Nutrient Requirements of Poultry, 1994) recommendations had better effects on antioxidant and immune capacity, including resisting viruses and decreasing the abnormal proliferation of cells. As well as this, it also had better effects on maintaining the homeostasis of the body. ABSTRACT: This study aimed to determine the effects of different dietary threonine levels on the antioxidant and immune capacity and the immunity of broilers. A total of 432 one-day-old Arbor Acres (AA) broilers were randomly assigned to 4 groups, each with 6 replicates of 18 broilers. The amount of dietary threonine in the four treatments reached 85%, 100%, 125%, and 150% of the NRC (Nutrient Requirements of Poultry, 1994) recommendation for broilers (marked as THR85, THR100, THR125, and THR150). After 42 days of feeding, the cecum contents and jejunum mucosa were collected for metabolic analysis and transcriptional sequencing. The results indicated that under the condition of regular and non-disease growth of broilers, compared with that of the THR85 and THR150 groups, the metabolic profile of the THR125 group was significantly higher than that of the standard requirement group. Compared with the THR100 group, the THR125 group improved antioxidant ability and immunity of broilers and enhanced the ability of resisting viruses. The antioxidant gene CAT was upregulated. PLCD1, which is involved in immune signal transduction and plays a role in cancer suppression, was also upregulated. Carcinogenic or indirect genes PKM2, ACY1, HK2, and TBXA2 were down-regulated. The genes GPT2, glude2, and G6PC, which played an important role in maintaining homeostasis, were up-regulated. Therefore, the present study suggests that 125% of the NRC recommendations for Thr level had better effects on antioxidant and immune capacity, as well as maintaining the homeostasis of the body.