Cargando…
The G119S Acetylcholinesterase (Ace-1) Target Site Mutation Confers Carbamate Resistance in the Major Malaria Vector Anopheles gambiae from Cameroon: A Challenge for the Coming IRS Implementation
Growing resistance is reported to carbamate insecticides in malaria vectors in Cameroon. However, the contribution of acetylcholinesterase (Ace-1) to this resistance remains uncharacterised. Here, we established that the G119S mutation is driving resistance to carbamates in Anopheles gambiae populat...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826778/ https://www.ncbi.nlm.nih.gov/pubmed/31614683 http://dx.doi.org/10.3390/genes10100790 |
_version_ | 1783465171238256640 |
---|---|
author | Elanga-Ndille, Emmanuel Nouage, Lynda Ndo, Cyrille Binyang, Achille Assatse, Tatiane Nguiffo-Nguete, Daniel Djonabaye, Doumani Irving, Helen Tene-Fossog, Billy Wondji, Charles S. |
author_facet | Elanga-Ndille, Emmanuel Nouage, Lynda Ndo, Cyrille Binyang, Achille Assatse, Tatiane Nguiffo-Nguete, Daniel Djonabaye, Doumani Irving, Helen Tene-Fossog, Billy Wondji, Charles S. |
author_sort | Elanga-Ndille, Emmanuel |
collection | PubMed |
description | Growing resistance is reported to carbamate insecticides in malaria vectors in Cameroon. However, the contribution of acetylcholinesterase (Ace-1) to this resistance remains uncharacterised. Here, we established that the G119S mutation is driving resistance to carbamates in Anopheles gambiae populations from Cameroon. Insecticide bioassay on field-collected mosquitoes from Bankeng, a locality in southern Cameroon, showed high resistance to the carbamates bendiocarb (64.8% ± 3.5% mortality) and propoxur (55.71% ± 2.9%) but a full susceptibility to the organophosphate fenitrothion. The TaqMan genotyping of the G119S mutation in field-collected adults revealed the presence of this resistance allele (39%). A significant correlation was observed between the Ace-1(R) and carbamate resistance at allelic ((bendiocarb; odds ratio (OR) = 75.9; p < 0.0001) and (propoxur; OR = 1514; p < 0.0001)) and genotypic (homozygote resistant vs. homozygote susceptible (bendiocarb; OR = 120.8; p < 0.0001) and (propoxur; OR = 3277; p < 0.0001)) levels. Furthermore, the presence of the mutation was confirmed by sequencing an Ace-1 portion flanking codon 119. The cloning of this fragment revealed a likely duplication of Ace-1 in Cameroon as mosquitoes exhibited at least three distinct haplotypes. Phylogenetic analyses showed that the predominant Ace-1(R) allele is identical to that from West Africa suggesting a recent introduction of this allele in Central Africa from the West. The spread of this Ace-1(R) represents a serious challenge to future implementation of indoor residual spraying (IRS)-based interventions using carbamates or organophosphates in Cameroon. |
format | Online Article Text |
id | pubmed-6826778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68267782019-11-18 The G119S Acetylcholinesterase (Ace-1) Target Site Mutation Confers Carbamate Resistance in the Major Malaria Vector Anopheles gambiae from Cameroon: A Challenge for the Coming IRS Implementation Elanga-Ndille, Emmanuel Nouage, Lynda Ndo, Cyrille Binyang, Achille Assatse, Tatiane Nguiffo-Nguete, Daniel Djonabaye, Doumani Irving, Helen Tene-Fossog, Billy Wondji, Charles S. Genes (Basel) Article Growing resistance is reported to carbamate insecticides in malaria vectors in Cameroon. However, the contribution of acetylcholinesterase (Ace-1) to this resistance remains uncharacterised. Here, we established that the G119S mutation is driving resistance to carbamates in Anopheles gambiae populations from Cameroon. Insecticide bioassay on field-collected mosquitoes from Bankeng, a locality in southern Cameroon, showed high resistance to the carbamates bendiocarb (64.8% ± 3.5% mortality) and propoxur (55.71% ± 2.9%) but a full susceptibility to the organophosphate fenitrothion. The TaqMan genotyping of the G119S mutation in field-collected adults revealed the presence of this resistance allele (39%). A significant correlation was observed between the Ace-1(R) and carbamate resistance at allelic ((bendiocarb; odds ratio (OR) = 75.9; p < 0.0001) and (propoxur; OR = 1514; p < 0.0001)) and genotypic (homozygote resistant vs. homozygote susceptible (bendiocarb; OR = 120.8; p < 0.0001) and (propoxur; OR = 3277; p < 0.0001)) levels. Furthermore, the presence of the mutation was confirmed by sequencing an Ace-1 portion flanking codon 119. The cloning of this fragment revealed a likely duplication of Ace-1 in Cameroon as mosquitoes exhibited at least three distinct haplotypes. Phylogenetic analyses showed that the predominant Ace-1(R) allele is identical to that from West Africa suggesting a recent introduction of this allele in Central Africa from the West. The spread of this Ace-1(R) represents a serious challenge to future implementation of indoor residual spraying (IRS)-based interventions using carbamates or organophosphates in Cameroon. MDPI 2019-10-11 /pmc/articles/PMC6826778/ /pubmed/31614683 http://dx.doi.org/10.3390/genes10100790 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Elanga-Ndille, Emmanuel Nouage, Lynda Ndo, Cyrille Binyang, Achille Assatse, Tatiane Nguiffo-Nguete, Daniel Djonabaye, Doumani Irving, Helen Tene-Fossog, Billy Wondji, Charles S. The G119S Acetylcholinesterase (Ace-1) Target Site Mutation Confers Carbamate Resistance in the Major Malaria Vector Anopheles gambiae from Cameroon: A Challenge for the Coming IRS Implementation |
title | The G119S Acetylcholinesterase (Ace-1) Target Site Mutation Confers Carbamate Resistance in the Major Malaria Vector Anopheles gambiae from Cameroon: A Challenge for the Coming IRS Implementation |
title_full | The G119S Acetylcholinesterase (Ace-1) Target Site Mutation Confers Carbamate Resistance in the Major Malaria Vector Anopheles gambiae from Cameroon: A Challenge for the Coming IRS Implementation |
title_fullStr | The G119S Acetylcholinesterase (Ace-1) Target Site Mutation Confers Carbamate Resistance in the Major Malaria Vector Anopheles gambiae from Cameroon: A Challenge for the Coming IRS Implementation |
title_full_unstemmed | The G119S Acetylcholinesterase (Ace-1) Target Site Mutation Confers Carbamate Resistance in the Major Malaria Vector Anopheles gambiae from Cameroon: A Challenge for the Coming IRS Implementation |
title_short | The G119S Acetylcholinesterase (Ace-1) Target Site Mutation Confers Carbamate Resistance in the Major Malaria Vector Anopheles gambiae from Cameroon: A Challenge for the Coming IRS Implementation |
title_sort | g119s acetylcholinesterase (ace-1) target site mutation confers carbamate resistance in the major malaria vector anopheles gambiae from cameroon: a challenge for the coming irs implementation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826778/ https://www.ncbi.nlm.nih.gov/pubmed/31614683 http://dx.doi.org/10.3390/genes10100790 |
work_keys_str_mv | AT elangandilleemmanuel theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT nouagelynda theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT ndocyrille theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT binyangachille theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT assatsetatiane theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT nguiffonguetedaniel theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT djonabayedoumani theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT irvinghelen theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT tenefossogbilly theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT wondjicharless theg119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT elangandilleemmanuel g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT nouagelynda g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT ndocyrille g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT binyangachille g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT assatsetatiane g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT nguiffonguetedaniel g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT djonabayedoumani g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT irvinghelen g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT tenefossogbilly g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation AT wondjicharless g119sacetylcholinesteraseace1targetsitemutationconferscarbamateresistanceinthemajormalariavectoranophelesgambiaefromcameroonachallengeforthecomingirsimplementation |