The Anti-Oxidation and Mechanism of Essential Oil of Paederia scandens in the NAFLD Model of Chicken

SIMPLE SUMMARY: The essential oil of Paederia scandens can remedy non-alcoholic fatty liver disease of chicken, but the mechanisms remain unclear. In this study, proteomics technology was used to declare the anti-non-alcoholic fatty liver disease mechanism of Paederia scandens essential oil. The results show that the essential oil of Paederia scandens significant decreased the oxidative stress of non-alcoholic fatty liver disease in chicken, which was mainly due to the center regulation protein of HSP7C being significantly inhibited. ABSTRACT: The aim of the study is to determine the underlying pathogenic mechanisms of oxidative stress and detect the anti-oxidative target of essential oil of Paederia scandens in non-alcoholic fatty liver disease (NAFLD). Chicken NAFLD was modeled by feeding with a high-capacity diet and Paederia scandens essential oil was used to treat the disease. The levels of hepatic reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), and the differential proteins and network of protein–protein interactions were investigated in model and drug-treated groups. The results showed that essential oil of Paederia scandens down regulated the hepatic ROS and MDA level significantly (p < 0.05 and 0.01, respectively). The heat shock cognate 71 kDa protein (HSP7C) was down regulated significantly, which was in the center of the network and interacted with 22 other proteins. The results showed that oxidative stress played an important role in the pathogenesis of chicken NAFLD. The essential oil of Paederia scandens showed good anti-oxidation activity by down regulating the HSP7C protein, which can be used as a potential therapeutic target in chicken NAFLD.