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C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration

Tobacco smoking is the major cause of disability and death in the United States and around the world. In addition, tobacco dependence and addiction express themselves as complex behaviors involving an interplay of genetics, environment, and psychological state. Mouse genetic studies could potentiall...

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Autores principales: Akinola, Lois S., Mckiver, Bryan, Toma, Wisam, Zhu, Andy Z. X., Tyndale, Rachel F., Kumar, Vivek, Damaj, M. Imad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827359/
https://www.ncbi.nlm.nih.gov/pubmed/31546627
http://dx.doi.org/10.3390/brainsci9100244
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author Akinola, Lois S.
Mckiver, Bryan
Toma, Wisam
Zhu, Andy Z. X.
Tyndale, Rachel F.
Kumar, Vivek
Damaj, M. Imad
author_facet Akinola, Lois S.
Mckiver, Bryan
Toma, Wisam
Zhu, Andy Z. X.
Tyndale, Rachel F.
Kumar, Vivek
Damaj, M. Imad
author_sort Akinola, Lois S.
collection PubMed
description Tobacco smoking is the major cause of disability and death in the United States and around the world. In addition, tobacco dependence and addiction express themselves as complex behaviors involving an interplay of genetics, environment, and psychological state. Mouse genetic studies could potentially elucidate the novel genes and/or gene networks regulating various aspects of nicotine dependence. Using the closely related C57BL/6 (B6) mice substrains, recent reports have noted phenotypic differences within C57BL/6J (B6J) and C57BL/6N (B6N) mice for some drugs of abuse: alcohol, opiates, and cocaine. However, the differences in nicotine’s effects have not yet been described in these substrains. We examined the phenotypic differences in these substrains following the acute and repeated administration of nicotine in several pharmacological measures, including locomotion (after acute and repeated exposure), body temperature, nociception, and anxiety-like behaviors. We report substrain differences in the pharmacological effects of acute and repeated nicotine administration in the B6 substrains. Overall, we show enhanced nicotine sensitivity to locomotion, hypothermia, antinociception, and anxiety-like behaviors in the B6J mouse substrain compared to B6N. In the repeated administration paradigm, both the B6N and B6J substrains showed no sensitized locomotor responses after repeated exposure to nicotine at the two doses tested. This study thus provides evidence that the B6 mouse substrains may be useful for genetic studies to elucidate some of the genetic variants involved in tobacco dependence and addiction.
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spelling pubmed-68273592019-11-18 C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration Akinola, Lois S. Mckiver, Bryan Toma, Wisam Zhu, Andy Z. X. Tyndale, Rachel F. Kumar, Vivek Damaj, M. Imad Brain Sci Article Tobacco smoking is the major cause of disability and death in the United States and around the world. In addition, tobacco dependence and addiction express themselves as complex behaviors involving an interplay of genetics, environment, and psychological state. Mouse genetic studies could potentially elucidate the novel genes and/or gene networks regulating various aspects of nicotine dependence. Using the closely related C57BL/6 (B6) mice substrains, recent reports have noted phenotypic differences within C57BL/6J (B6J) and C57BL/6N (B6N) mice for some drugs of abuse: alcohol, opiates, and cocaine. However, the differences in nicotine’s effects have not yet been described in these substrains. We examined the phenotypic differences in these substrains following the acute and repeated administration of nicotine in several pharmacological measures, including locomotion (after acute and repeated exposure), body temperature, nociception, and anxiety-like behaviors. We report substrain differences in the pharmacological effects of acute and repeated nicotine administration in the B6 substrains. Overall, we show enhanced nicotine sensitivity to locomotion, hypothermia, antinociception, and anxiety-like behaviors in the B6J mouse substrain compared to B6N. In the repeated administration paradigm, both the B6N and B6J substrains showed no sensitized locomotor responses after repeated exposure to nicotine at the two doses tested. This study thus provides evidence that the B6 mouse substrains may be useful for genetic studies to elucidate some of the genetic variants involved in tobacco dependence and addiction. MDPI 2019-09-21 /pmc/articles/PMC6827359/ /pubmed/31546627 http://dx.doi.org/10.3390/brainsci9100244 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akinola, Lois S.
Mckiver, Bryan
Toma, Wisam
Zhu, Andy Z. X.
Tyndale, Rachel F.
Kumar, Vivek
Damaj, M. Imad
C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration
title C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration
title_full C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration
title_fullStr C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration
title_full_unstemmed C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration
title_short C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration
title_sort c57bl/6 substrain differences in pharmacological effects after acute and repeated nicotine administration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827359/
https://www.ncbi.nlm.nih.gov/pubmed/31546627
http://dx.doi.org/10.3390/brainsci9100244
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