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Physicochemical Properties of Hyaluronic Acid–Based Lubricant Eye Drops

PURPOSE: To assess the physicochemical properties of hyaluronic acid (HA)-based artificial tears. METHODS: The average molecular weight (MW) and polydispersion index (PDI) of HA in 18 commercially available artificial tears were determined by light scattering/high-performance liquid chromatography....

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Autores principales: Aragona, Pasquale, Simmons, Peter A., Wang, Hongpeng, Wang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827422/
https://www.ncbi.nlm.nih.gov/pubmed/31695963
http://dx.doi.org/10.1167/tvst.8.6.2
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author Aragona, Pasquale
Simmons, Peter A.
Wang, Hongpeng
Wang, Tao
author_facet Aragona, Pasquale
Simmons, Peter A.
Wang, Hongpeng
Wang, Tao
author_sort Aragona, Pasquale
collection PubMed
description PURPOSE: To assess the physicochemical properties of hyaluronic acid (HA)-based artificial tears. METHODS: The average molecular weight (MW) and polydispersion index (PDI) of HA in 18 commercially available artificial tears were determined by light scattering/high-performance liquid chromatography. Osmolality, pH, viscosity, and sodium concentration were determined using an osmometer, pH meter, rheometer, and inductively coupled plasma mass spectrometer, respectively. RESULTS: The MW of HA varied considerably between formulations. The PDI was >2.0 in two formulations (2.28 and 4.94), suggesting the presence of a copolymer and/or HA size variability. Three formulations exhibited viscosity exceeding the blur threshold at different shear rates. Viscosity at low shear rates was generally highest in formulations containing high-MW HA. Correlations were found between observed viscosity and a predictive/calculated value, except for four copolymer-containing formulations, and osmolality (range, 154–335 mOsm/kg) and sodium concentration (range, 22–183 mM), with two exceptions. Compared with organic osmolytes, adding sodium decreased viscosity, particularly at lower shear rates. CONCLUSIONS: In the context of the literature, our findings suggest that for most patients with dry eye disease, the ideal HA-based artificial tear should include high-MW HA with a low PDI and exhibit enhanced viscosity at low shear rate (without exceeding the blur threshold). The inclusion of synergistic copolymers and a low sodium concentration may increase viscosity, but whether any of these physicochemical properties or correlations can predict clinical efficacy will require further investigation. TRANSLATIONAL RELEVANCE: Understanding the properties of HA-based artificial tears will support the development of unique formulations that target specific ocular surface conditions.
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spelling pubmed-68274222019-11-06 Physicochemical Properties of Hyaluronic Acid–Based Lubricant Eye Drops Aragona, Pasquale Simmons, Peter A. Wang, Hongpeng Wang, Tao Transl Vis Sci Technol Articles PURPOSE: To assess the physicochemical properties of hyaluronic acid (HA)-based artificial tears. METHODS: The average molecular weight (MW) and polydispersion index (PDI) of HA in 18 commercially available artificial tears were determined by light scattering/high-performance liquid chromatography. Osmolality, pH, viscosity, and sodium concentration were determined using an osmometer, pH meter, rheometer, and inductively coupled plasma mass spectrometer, respectively. RESULTS: The MW of HA varied considerably between formulations. The PDI was >2.0 in two formulations (2.28 and 4.94), suggesting the presence of a copolymer and/or HA size variability. Three formulations exhibited viscosity exceeding the blur threshold at different shear rates. Viscosity at low shear rates was generally highest in formulations containing high-MW HA. Correlations were found between observed viscosity and a predictive/calculated value, except for four copolymer-containing formulations, and osmolality (range, 154–335 mOsm/kg) and sodium concentration (range, 22–183 mM), with two exceptions. Compared with organic osmolytes, adding sodium decreased viscosity, particularly at lower shear rates. CONCLUSIONS: In the context of the literature, our findings suggest that for most patients with dry eye disease, the ideal HA-based artificial tear should include high-MW HA with a low PDI and exhibit enhanced viscosity at low shear rate (without exceeding the blur threshold). The inclusion of synergistic copolymers and a low sodium concentration may increase viscosity, but whether any of these physicochemical properties or correlations can predict clinical efficacy will require further investigation. TRANSLATIONAL RELEVANCE: Understanding the properties of HA-based artificial tears will support the development of unique formulations that target specific ocular surface conditions. The Association for Research in Vision and Ophthalmology 2019-11-01 /pmc/articles/PMC6827422/ /pubmed/31695963 http://dx.doi.org/10.1167/tvst.8.6.2 Text en Copyright 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Articles
Aragona, Pasquale
Simmons, Peter A.
Wang, Hongpeng
Wang, Tao
Physicochemical Properties of Hyaluronic Acid–Based Lubricant Eye Drops
title Physicochemical Properties of Hyaluronic Acid–Based Lubricant Eye Drops
title_full Physicochemical Properties of Hyaluronic Acid–Based Lubricant Eye Drops
title_fullStr Physicochemical Properties of Hyaluronic Acid–Based Lubricant Eye Drops
title_full_unstemmed Physicochemical Properties of Hyaluronic Acid–Based Lubricant Eye Drops
title_short Physicochemical Properties of Hyaluronic Acid–Based Lubricant Eye Drops
title_sort physicochemical properties of hyaluronic acid–based lubricant eye drops
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827422/
https://www.ncbi.nlm.nih.gov/pubmed/31695963
http://dx.doi.org/10.1167/tvst.8.6.2
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