Off-line and on-line optical monitoring of microalgal growth

The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, using classic off-line optical techniques (optical density and fluorescence) and on-line analysis of digital images. In this study, we found that the chlorophyll fluorescence ratio F(685)/F(740) has a linear corr...

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Autores principales: Lazcano-Hernández, Hugo-Enrique, Aguilar, Gabriela, Dzul-Cetz, Gabriela Antonia, Patiño, Rodrigo, Arellano-Verdejo, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827447/
https://www.ncbi.nlm.nih.gov/pubmed/31695966
http://dx.doi.org/10.7717/peerj.7956
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author Lazcano-Hernández, Hugo-Enrique
Aguilar, Gabriela
Dzul-Cetz, Gabriela Antonia
Patiño, Rodrigo
Arellano-Verdejo, Javier
author_facet Lazcano-Hernández, Hugo-Enrique
Aguilar, Gabriela
Dzul-Cetz, Gabriela Antonia
Patiño, Rodrigo
Arellano-Verdejo, Javier
author_sort Lazcano-Hernández, Hugo-Enrique
collection PubMed
description The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, using classic off-line optical techniques (optical density and fluorescence) and on-line analysis of digital images. In this study, we found that the chlorophyll fluorescence ratio F(685)/F(740) has a linear correlation with the logarithmic concentration of microalgae. By using digital images, the biomass concentration correlated with the luminosity of the images through an exponential equation and the length of penetration of a super luminescent blue beam (λ = 440 nm) through an inversely proportional function. The outcomes of this study are useful to monitor both research and industrial microalgae cultures.
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spelling pubmed-68274472019-11-06 Off-line and on-line optical monitoring of microalgal growth Lazcano-Hernández, Hugo-Enrique Aguilar, Gabriela Dzul-Cetz, Gabriela Antonia Patiño, Rodrigo Arellano-Verdejo, Javier PeerJ Bioengineering The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, using classic off-line optical techniques (optical density and fluorescence) and on-line analysis of digital images. In this study, we found that the chlorophyll fluorescence ratio F(685)/F(740) has a linear correlation with the logarithmic concentration of microalgae. By using digital images, the biomass concentration correlated with the luminosity of the images through an exponential equation and the length of penetration of a super luminescent blue beam (λ = 440 nm) through an inversely proportional function. The outcomes of this study are useful to monitor both research and industrial microalgae cultures. PeerJ Inc. 2019-11-01 /pmc/articles/PMC6827447/ /pubmed/31695966 http://dx.doi.org/10.7717/peerj.7956 Text en © 2019 Lazcano-Hernández et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioengineering
Lazcano-Hernández, Hugo-Enrique
Aguilar, Gabriela
Dzul-Cetz, Gabriela Antonia
Patiño, Rodrigo
Arellano-Verdejo, Javier
Off-line and on-line optical monitoring of microalgal growth
title Off-line and on-line optical monitoring of microalgal growth
title_full Off-line and on-line optical monitoring of microalgal growth
title_fullStr Off-line and on-line optical monitoring of microalgal growth
title_full_unstemmed Off-line and on-line optical monitoring of microalgal growth
title_short Off-line and on-line optical monitoring of microalgal growth
title_sort off-line and on-line optical monitoring of microalgal growth
topic Bioengineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827447/
https://www.ncbi.nlm.nih.gov/pubmed/31695966
http://dx.doi.org/10.7717/peerj.7956
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