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Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China

Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G. duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3–12 months ol...

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Autores principales: Dan, Jiaming, Zhang, Xueping, Ren, Zhihua, Wang, Liqin, Cao, Suizhong, Shen, Liuhong, Deng, Junliang, Zuo, Zhicai, Yu, Shumin, Wang, Ya, Ma, Xiaoping, Liu, Haifeng, Zhou, Ziyao, Hu, Yanchun, Fu, Hualin, He, Changliang, Geng, Yi, Gu, Xiaobin, Peng, Guangneng, Zhong, Zhijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827885/
https://www.ncbi.nlm.nih.gov/pubmed/31682629
http://dx.doi.org/10.1371/journal.pone.0224627
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author Dan, Jiaming
Zhang, Xueping
Ren, Zhihua
Wang, Liqin
Cao, Suizhong
Shen, Liuhong
Deng, Junliang
Zuo, Zhicai
Yu, Shumin
Wang, Ya
Ma, Xiaoping
Liu, Haifeng
Zhou, Ziyao
Hu, Yanchun
Fu, Hualin
He, Changliang
Geng, Yi
Gu, Xiaobin
Peng, Guangneng
Zhong, Zhijun
author_facet Dan, Jiaming
Zhang, Xueping
Ren, Zhihua
Wang, Liqin
Cao, Suizhong
Shen, Liuhong
Deng, Junliang
Zuo, Zhicai
Yu, Shumin
Wang, Ya
Ma, Xiaoping
Liu, Haifeng
Zhou, Ziyao
Hu, Yanchun
Fu, Hualin
He, Changliang
Geng, Yi
Gu, Xiaobin
Peng, Guangneng
Zhong, Zhijun
author_sort Dan, Jiaming
collection PubMed
description Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G. duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3–12 months old) from 10 farms, including 4 intensive feeding farms and 6 free-ranging farms. The overall infection rate of G. duodenalis was 41.2% (126/306) based on the PCR results at any of the three genetic loci: beta-giardin (bg), triose-phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Giardia duodenalis assemblages E (n = 115, 91.3%), A (n = 3, 2.4%), and A mixed with E (n = 8, 6.3%) were identified among the 126 positive specimens. Multilocus sequence typing of G. duodenalis revealed 34 assemblage E multilocus genotypes (MLGs), 1 assemblage A MLG and 7 mixed assemblage (A and E) MLGs. The eBURST data showed a high degree of genetic diversity within assemblage E MLGs. The phylogenetic tree revealed that MLG E3 was the primary MLG subtype in Sichuan province and also the most widely distributed in China.
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spelling pubmed-68278852019-11-12 Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China Dan, Jiaming Zhang, Xueping Ren, Zhihua Wang, Liqin Cao, Suizhong Shen, Liuhong Deng, Junliang Zuo, Zhicai Yu, Shumin Wang, Ya Ma, Xiaoping Liu, Haifeng Zhou, Ziyao Hu, Yanchun Fu, Hualin He, Changliang Geng, Yi Gu, Xiaobin Peng, Guangneng Zhong, Zhijun PLoS One Research Article Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G. duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3–12 months old) from 10 farms, including 4 intensive feeding farms and 6 free-ranging farms. The overall infection rate of G. duodenalis was 41.2% (126/306) based on the PCR results at any of the three genetic loci: beta-giardin (bg), triose-phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Giardia duodenalis assemblages E (n = 115, 91.3%), A (n = 3, 2.4%), and A mixed with E (n = 8, 6.3%) were identified among the 126 positive specimens. Multilocus sequence typing of G. duodenalis revealed 34 assemblage E multilocus genotypes (MLGs), 1 assemblage A MLG and 7 mixed assemblage (A and E) MLGs. The eBURST data showed a high degree of genetic diversity within assemblage E MLGs. The phylogenetic tree revealed that MLG E3 was the primary MLG subtype in Sichuan province and also the most widely distributed in China. Public Library of Science 2019-11-04 /pmc/articles/PMC6827885/ /pubmed/31682629 http://dx.doi.org/10.1371/journal.pone.0224627 Text en © 2019 Dan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dan, Jiaming
Zhang, Xueping
Ren, Zhihua
Wang, Liqin
Cao, Suizhong
Shen, Liuhong
Deng, Junliang
Zuo, Zhicai
Yu, Shumin
Wang, Ya
Ma, Xiaoping
Liu, Haifeng
Zhou, Ziyao
Hu, Yanchun
Fu, Hualin
He, Changliang
Geng, Yi
Gu, Xiaobin
Peng, Guangneng
Zhong, Zhijun
Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China
title Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China
title_full Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China
title_fullStr Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China
title_full_unstemmed Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China
title_short Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China
title_sort occurrence and multilocus genotyping of giardia duodenalis from post-weaned dairy calves in sichuan province, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827885/
https://www.ncbi.nlm.nih.gov/pubmed/31682629
http://dx.doi.org/10.1371/journal.pone.0224627
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