Adaptation to mutational inactivation of an essential gene converges to an accessible suboptimal fitness peak

The mechanisms of adaptation to inactivation of essential genes remain unknown. Here we inactivate E. coli dihydrofolate reductase (DHFR) by introducing D27G,N,F chromosomal mutations in a key catalytic residue with subsequent adaptation by an automated serial transfer protocol. The partial reversal...

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Detalles Bibliográficos
Autores principales: Rodrigues, João V, Shakhnovich, Eugene I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Computational and Systems Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6828540/
https://www.ncbi.nlm.nih.gov/pubmed/31573512
http://dx.doi.org/10.7554/eLife.50509

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6828540/
https://www.ncbi.nlm.nih.gov/pubmed/31573512
http://dx.doi.org/10.7554/eLife.50509

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