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Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis
Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research, the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6828593/ https://www.ncbi.nlm.nih.gov/pubmed/31693013 http://dx.doi.org/10.4252/wjsc.v11.i10.803 |
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author | Kim, Jeong Ah Hong, Soohyun Rhee, Won Jong |
author_facet | Kim, Jeong Ah Hong, Soohyun Rhee, Won Jong |
author_sort | Kim, Jeong Ah |
collection | PubMed |
description | Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research, the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell microenvironment and the limited availability of high-throughput systems. Recently, researchers have been actively developing and evaluating three-dimensional (3D) cell culture-based platforms using microfluidic technologies, such as organ-on-a-chip and organoid-on-a-chip platforms, and they have achieved promising breakthroughs in stem cell engineering. In this review, we start with a comprehensive discussion on the importance of microfluidic 3D cell culture techniques in stem cell research and their technical strategies in the field of drug discovery. In a subsequent section, we discuss microfluidic 3D cell culture techniques for high-throughput analysis for use in stem cell research. In addition, some potential and practical applications of organ-on-a-chip or organoid-on-a-chip platforms using stem cells as drug screening and disease models are highlighted. |
format | Online Article Text |
id | pubmed-6828593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-68285932019-11-05 Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis Kim, Jeong Ah Hong, Soohyun Rhee, Won Jong World J Stem Cells Review Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research, the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell microenvironment and the limited availability of high-throughput systems. Recently, researchers have been actively developing and evaluating three-dimensional (3D) cell culture-based platforms using microfluidic technologies, such as organ-on-a-chip and organoid-on-a-chip platforms, and they have achieved promising breakthroughs in stem cell engineering. In this review, we start with a comprehensive discussion on the importance of microfluidic 3D cell culture techniques in stem cell research and their technical strategies in the field of drug discovery. In a subsequent section, we discuss microfluidic 3D cell culture techniques for high-throughput analysis for use in stem cell research. In addition, some potential and practical applications of organ-on-a-chip or organoid-on-a-chip platforms using stem cells as drug screening and disease models are highlighted. Baishideng Publishing Group Inc 2019-10-26 2019-10-26 /pmc/articles/PMC6828593/ /pubmed/31693013 http://dx.doi.org/10.4252/wjsc.v11.i10.803 Text en ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Review Kim, Jeong Ah Hong, Soohyun Rhee, Won Jong Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis |
title | Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis |
title_full | Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis |
title_fullStr | Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis |
title_full_unstemmed | Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis |
title_short | Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis |
title_sort | microfluidic three-dimensional cell culture of stem cells for high-throughput analysis |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6828593/ https://www.ncbi.nlm.nih.gov/pubmed/31693013 http://dx.doi.org/10.4252/wjsc.v11.i10.803 |
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