Cargando…

Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis

Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research, the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jeong Ah, Hong, Soohyun, Rhee, Won Jong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6828593/
https://www.ncbi.nlm.nih.gov/pubmed/31693013
http://dx.doi.org/10.4252/wjsc.v11.i10.803
_version_ 1783465381084528640
author Kim, Jeong Ah
Hong, Soohyun
Rhee, Won Jong
author_facet Kim, Jeong Ah
Hong, Soohyun
Rhee, Won Jong
author_sort Kim, Jeong Ah
collection PubMed
description Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research, the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell microenvironment and the limited availability of high-throughput systems. Recently, researchers have been actively developing and evaluating three-dimensional (3D) cell culture-based platforms using microfluidic technologies, such as organ-on-a-chip and organoid-on-a-chip platforms, and they have achieved promising breakthroughs in stem cell engineering. In this review, we start with a comprehensive discussion on the importance of microfluidic 3D cell culture techniques in stem cell research and their technical strategies in the field of drug discovery. In a subsequent section, we discuss microfluidic 3D cell culture techniques for high-throughput analysis for use in stem cell research. In addition, some potential and practical applications of organ-on-a-chip or organoid-on-a-chip platforms using stem cells as drug screening and disease models are highlighted.
format Online
Article
Text
id pubmed-6828593
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Baishideng Publishing Group Inc
record_format MEDLINE/PubMed
spelling pubmed-68285932019-11-05 Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis Kim, Jeong Ah Hong, Soohyun Rhee, Won Jong World J Stem Cells Review Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research, the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell microenvironment and the limited availability of high-throughput systems. Recently, researchers have been actively developing and evaluating three-dimensional (3D) cell culture-based platforms using microfluidic technologies, such as organ-on-a-chip and organoid-on-a-chip platforms, and they have achieved promising breakthroughs in stem cell engineering. In this review, we start with a comprehensive discussion on the importance of microfluidic 3D cell culture techniques in stem cell research and their technical strategies in the field of drug discovery. In a subsequent section, we discuss microfluidic 3D cell culture techniques for high-throughput analysis for use in stem cell research. In addition, some potential and practical applications of organ-on-a-chip or organoid-on-a-chip platforms using stem cells as drug screening and disease models are highlighted. Baishideng Publishing Group Inc 2019-10-26 2019-10-26 /pmc/articles/PMC6828593/ /pubmed/31693013 http://dx.doi.org/10.4252/wjsc.v11.i10.803 Text en ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Review
Kim, Jeong Ah
Hong, Soohyun
Rhee, Won Jong
Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis
title Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis
title_full Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis
title_fullStr Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis
title_full_unstemmed Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis
title_short Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis
title_sort microfluidic three-dimensional cell culture of stem cells for high-throughput analysis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6828593/
https://www.ncbi.nlm.nih.gov/pubmed/31693013
http://dx.doi.org/10.4252/wjsc.v11.i10.803
work_keys_str_mv AT kimjeongah microfluidicthreedimensionalcellcultureofstemcellsforhighthroughputanalysis
AT hongsoohyun microfluidicthreedimensionalcellcultureofstemcellsforhighthroughputanalysis
AT rheewonjong microfluidicthreedimensionalcellcultureofstemcellsforhighthroughputanalysis