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Cutaneous candidiasis in Tehran-Iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic Candida species
BACKGROUND AND OBJECTIVES: Cutaneous candidiasis is a multipicture fungal infection caused by members of the genus Candida which is considered as a public health problem all over the world with urgency of effective treatment and control. This study was performed to analyze the clinical epidemiology...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829109/ https://www.ncbi.nlm.nih.gov/pubmed/31719957 |
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author | Sadeghi, Golnar Ebrahimi-Rad, Mina Shams-Ghahfarokhi, Masoomeh Jahanshiri, Zahra Ardakani, Esmat Mirabzadeh Eslamifar, Ali Mousavi, Seyed Fazlollah Razzaghi-Abyaneh, Mehdi |
author_facet | Sadeghi, Golnar Ebrahimi-Rad, Mina Shams-Ghahfarokhi, Masoomeh Jahanshiri, Zahra Ardakani, Esmat Mirabzadeh Eslamifar, Ali Mousavi, Seyed Fazlollah Razzaghi-Abyaneh, Mehdi |
author_sort | Sadeghi, Golnar |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: Cutaneous candidiasis is a multipicture fungal infection caused by members of the genus Candida which is considered as a public health problem all over the world with urgency of effective treatment and control. This study was performed to analyze the clinical epidemiology and molecular aspects of cutaneous candidiasis in Tehran-Iran in relation to antifungal susceptibility and virulence factors of etiologic Candida species. MATERIALS AND METHODS: Candida species were isolated from skin (27.3%) and nail scrapings (72.7%) of suspected patients and identified by ITS sequencing. Phylogeny of the isolates was evaluated using multilocus sequence typing (MLST) and antifungal susceptibility and virulence factors of the isolates were determined in relation to clinical presentation. RESULTS: Candida albicans was the most prevalent species (39.8%), followed by C. parapsilosis (32.9%), C. orthopsilosis (10.4%), C. tropicalis (7.9%), C. glabrata and C. guilliermondii, each (4.5%). Molecular typing of 35 C. albicans isolates by MLST revealed 28 novel sequence types with 11 singletons with 80.0% new diploid sequence types (DSTs). Majority of the isolates were susceptible to amphotericin B (91.5%), followed by posaconazole (90.3%), fluconazole (84.3%), itraconazole (74.1%), caspofungin (53.6%), and voriconazole (26.8%). Biofilm formation, yeast-to-hyphae transformation and phospholipase activity were reported species-dependent. CONCLUSION: Our results demonstrated clinical epidemiology of various Candida species from cutaneous candidiasis distributed in new molecular types with increasing importance of drug resistant of non-albicans Candida species. Our results showed that drug susceptibility and genetic variability of Candida species may be attributed to their clinical features and source of isolation. |
format | Online Article Text |
id | pubmed-6829109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-68291092019-11-12 Cutaneous candidiasis in Tehran-Iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic Candida species Sadeghi, Golnar Ebrahimi-Rad, Mina Shams-Ghahfarokhi, Masoomeh Jahanshiri, Zahra Ardakani, Esmat Mirabzadeh Eslamifar, Ali Mousavi, Seyed Fazlollah Razzaghi-Abyaneh, Mehdi Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Cutaneous candidiasis is a multipicture fungal infection caused by members of the genus Candida which is considered as a public health problem all over the world with urgency of effective treatment and control. This study was performed to analyze the clinical epidemiology and molecular aspects of cutaneous candidiasis in Tehran-Iran in relation to antifungal susceptibility and virulence factors of etiologic Candida species. MATERIALS AND METHODS: Candida species were isolated from skin (27.3%) and nail scrapings (72.7%) of suspected patients and identified by ITS sequencing. Phylogeny of the isolates was evaluated using multilocus sequence typing (MLST) and antifungal susceptibility and virulence factors of the isolates were determined in relation to clinical presentation. RESULTS: Candida albicans was the most prevalent species (39.8%), followed by C. parapsilosis (32.9%), C. orthopsilosis (10.4%), C. tropicalis (7.9%), C. glabrata and C. guilliermondii, each (4.5%). Molecular typing of 35 C. albicans isolates by MLST revealed 28 novel sequence types with 11 singletons with 80.0% new diploid sequence types (DSTs). Majority of the isolates were susceptible to amphotericin B (91.5%), followed by posaconazole (90.3%), fluconazole (84.3%), itraconazole (74.1%), caspofungin (53.6%), and voriconazole (26.8%). Biofilm formation, yeast-to-hyphae transformation and phospholipase activity were reported species-dependent. CONCLUSION: Our results demonstrated clinical epidemiology of various Candida species from cutaneous candidiasis distributed in new molecular types with increasing importance of drug resistant of non-albicans Candida species. Our results showed that drug susceptibility and genetic variability of Candida species may be attributed to their clinical features and source of isolation. Tehran University of Medical Sciences 2019-08 /pmc/articles/PMC6829109/ /pubmed/31719957 Text en Copyright© 2019 Iranian Neuroscience Society http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Sadeghi, Golnar Ebrahimi-Rad, Mina Shams-Ghahfarokhi, Masoomeh Jahanshiri, Zahra Ardakani, Esmat Mirabzadeh Eslamifar, Ali Mousavi, Seyed Fazlollah Razzaghi-Abyaneh, Mehdi Cutaneous candidiasis in Tehran-Iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic Candida species |
title | Cutaneous candidiasis in Tehran-Iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic Candida species |
title_full | Cutaneous candidiasis in Tehran-Iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic Candida species |
title_fullStr | Cutaneous candidiasis in Tehran-Iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic Candida species |
title_full_unstemmed | Cutaneous candidiasis in Tehran-Iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic Candida species |
title_short | Cutaneous candidiasis in Tehran-Iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic Candida species |
title_sort | cutaneous candidiasis in tehran-iran: from epidemiology to multilocus sequence types, virulence factors and antifungal susceptibility of etiologic candida species |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829109/ https://www.ncbi.nlm.nih.gov/pubmed/31719957 |
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