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Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment
BACKGROUND AND OBJECTIVES: Fibrinolytic drugs are commonly used for fibrin clot lysis but due to their inappropriate side effects, as well as their high costs, using fibrinolytic enzymes has been paid attention. Bacterial sources of this enzyme are a good alternative for this purpose. The aim was fi...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829113/ https://www.ncbi.nlm.nih.gov/pubmed/31719965 |
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author | Nikkhoy, Zahra Motamedi, Hossein |
author_facet | Nikkhoy, Zahra Motamedi, Hossein |
author_sort | Nikkhoy, Zahra |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: Fibrinolytic drugs are commonly used for fibrin clot lysis but due to their inappropriate side effects, as well as their high costs, using fibrinolytic enzymes has been paid attention. Bacterial sources of this enzyme are a good alternative for this purpose. The aim was fibrinolysin production through screening of fibrinolysin producing bacteria from environmental samples. MATERIALS AND METHODS: Bacterial isolation was performed from different environmental samples and was screened based on sheep blood clot digestion and culture on plasma plate. The most potent isolate was optimized for different growth parameters including temperature, pH and fibrinolysin production at optimum growth conditions. The stability of produced enzyme at various temperatures and pH and treatment with MgSO(4), NiSO(4), SDS and EDTA was then investigated. Finally this isolate was identified based on the 16S rRNA sequencing. RESULTS: As a result, from 79 different isolates, the most potent fibrinolysin producer was identified as Alcaligenes faecalis strain 26. This isolate produced 12 mm halo zone on plasma plate. Its optimum growth temperature and pH was 43°C and 7, respectively. The produced enzyme had the best stability at pH 7 and was also active up to 60°C. The fibrinolytic activity of this isolate was reduced following treatment with MgSO(4), NiSO(4) and also with protease inhibitors, such as SDS and EDTA. CONCLUSION: Based on the obtained results it can be suggested that Alcaligenes faecalis strain 26 has appropriate efficiency for fibrinolysin production that can be used in food industry and medicine. |
format | Online Article Text |
id | pubmed-6829113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-68291132019-11-12 Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment Nikkhoy, Zahra Motamedi, Hossein Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Fibrinolytic drugs are commonly used for fibrin clot lysis but due to their inappropriate side effects, as well as their high costs, using fibrinolytic enzymes has been paid attention. Bacterial sources of this enzyme are a good alternative for this purpose. The aim was fibrinolysin production through screening of fibrinolysin producing bacteria from environmental samples. MATERIALS AND METHODS: Bacterial isolation was performed from different environmental samples and was screened based on sheep blood clot digestion and culture on plasma plate. The most potent isolate was optimized for different growth parameters including temperature, pH and fibrinolysin production at optimum growth conditions. The stability of produced enzyme at various temperatures and pH and treatment with MgSO(4), NiSO(4), SDS and EDTA was then investigated. Finally this isolate was identified based on the 16S rRNA sequencing. RESULTS: As a result, from 79 different isolates, the most potent fibrinolysin producer was identified as Alcaligenes faecalis strain 26. This isolate produced 12 mm halo zone on plasma plate. Its optimum growth temperature and pH was 43°C and 7, respectively. The produced enzyme had the best stability at pH 7 and was also active up to 60°C. The fibrinolytic activity of this isolate was reduced following treatment with MgSO(4), NiSO(4) and also with protease inhibitors, such as SDS and EDTA. CONCLUSION: Based on the obtained results it can be suggested that Alcaligenes faecalis strain 26 has appropriate efficiency for fibrinolysin production that can be used in food industry and medicine. Tehran University of Medical Sciences 2019-08 /pmc/articles/PMC6829113/ /pubmed/31719965 Text en Copyright© 2019 Iranian Neuroscience Society http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nikkhoy, Zahra Motamedi, Hossein Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment |
title | Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment |
title_full | Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment |
title_fullStr | Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment |
title_full_unstemmed | Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment |
title_short | Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment |
title_sort | fibrinolysin production by alcaligenes faecalis strain 26 isolated from environment |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829113/ https://www.ncbi.nlm.nih.gov/pubmed/31719965 |
work_keys_str_mv | AT nikkhoyzahra fibrinolysinproductionbyalcaligenesfaecalisstrain26isolatedfromenvironment AT motamedihossein fibrinolysinproductionbyalcaligenesfaecalisstrain26isolatedfromenvironment |