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Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China

Campylobacter jejuni (C. jejuni) is considered as an opportunistic zoonotic pathogen that may cause gastroenteritis in humans and other animals. Wild birds may be as potential vectors of C. jejuni around urban and suburban areas. Here, 520 samples were collected from 33 wild bird species in urban an...

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Autores principales: Du, Juan, Luo, Jing, Huang, Jingjing, Wang, Chengmin, Li, Meng, Wang, Bojun, Wang, Bo, Chang, Han, Ji, Jianwei, Sen, Keya, He, Hongxuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829156/
https://www.ncbi.nlm.nih.gov/pubmed/31736887
http://dx.doi.org/10.3389/fmicb.2019.02433
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author Du, Juan
Luo, Jing
Huang, Jingjing
Wang, Chengmin
Li, Meng
Wang, Bojun
Wang, Bo
Chang, Han
Ji, Jianwei
Sen, Keya
He, Hongxuan
author_facet Du, Juan
Luo, Jing
Huang, Jingjing
Wang, Chengmin
Li, Meng
Wang, Bojun
Wang, Bo
Chang, Han
Ji, Jianwei
Sen, Keya
He, Hongxuan
author_sort Du, Juan
collection PubMed
description Campylobacter jejuni (C. jejuni) is considered as an opportunistic zoonotic pathogen that may cause gastroenteritis in humans and other animals. Wild birds may be as potential vectors of C. jejuni around urban and suburban areas. Here, 520 samples were collected from 33 wild bird species in urban and suburban areas, Beijing. In total 57 C. jejuni were isolated from seven species. It was found that Nineteen (33.33%, 19/57) isolates were resistant to at least one of 11 antibiotics, especially streptomycin (36.84%) and four isolates resistant to all. Nineteen (33.33%, 19/57) isolates were multi-drug resistance. Multilocus sequence typing (MLST) analysis of the isolates showed that 36 different sequence types (STs) belonged to four Clonal complexes and unassigned. Twenty STs (55.56%) and six alleles among them were first detected. Virulence genes including flaA, cadF, and the cytolethal distending toxin (CDT) gene cluster, were detected in all isolates, but truncated cdt gene clusters only detected in the isolates from the crow, daurian jackdaw and silver pheasant. In conclusion, it was the first detection of C. jejuni involved truncated cdt gene clusters from the silver pheasant. These wild birds around urban and suburban areas may pose potential public health problems as reservoir vectors of C. jejuni.
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spelling pubmed-68291562019-11-15 Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China Du, Juan Luo, Jing Huang, Jingjing Wang, Chengmin Li, Meng Wang, Bojun Wang, Bo Chang, Han Ji, Jianwei Sen, Keya He, Hongxuan Front Microbiol Microbiology Campylobacter jejuni (C. jejuni) is considered as an opportunistic zoonotic pathogen that may cause gastroenteritis in humans and other animals. Wild birds may be as potential vectors of C. jejuni around urban and suburban areas. Here, 520 samples were collected from 33 wild bird species in urban and suburban areas, Beijing. In total 57 C. jejuni were isolated from seven species. It was found that Nineteen (33.33%, 19/57) isolates were resistant to at least one of 11 antibiotics, especially streptomycin (36.84%) and four isolates resistant to all. Nineteen (33.33%, 19/57) isolates were multi-drug resistance. Multilocus sequence typing (MLST) analysis of the isolates showed that 36 different sequence types (STs) belonged to four Clonal complexes and unassigned. Twenty STs (55.56%) and six alleles among them were first detected. Virulence genes including flaA, cadF, and the cytolethal distending toxin (CDT) gene cluster, were detected in all isolates, but truncated cdt gene clusters only detected in the isolates from the crow, daurian jackdaw and silver pheasant. In conclusion, it was the first detection of C. jejuni involved truncated cdt gene clusters from the silver pheasant. These wild birds around urban and suburban areas may pose potential public health problems as reservoir vectors of C. jejuni. Frontiers Media S.A. 2019-10-29 /pmc/articles/PMC6829156/ /pubmed/31736887 http://dx.doi.org/10.3389/fmicb.2019.02433 Text en Copyright © 2019 Du, Luo, Huang, Wang, Li, Wang, Wang, Chang, Ji, Sen and He. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Du, Juan
Luo, Jing
Huang, Jingjing
Wang, Chengmin
Li, Meng
Wang, Bojun
Wang, Bo
Chang, Han
Ji, Jianwei
Sen, Keya
He, Hongxuan
Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China
title Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China
title_full Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China
title_fullStr Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China
title_full_unstemmed Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China
title_short Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China
title_sort emergence of genetic diversity and multi-drug resistant campylobacter jejuni from wild birds in beijing, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829156/
https://www.ncbi.nlm.nih.gov/pubmed/31736887
http://dx.doi.org/10.3389/fmicb.2019.02433
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