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Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications

A high degree of developmental plasticity enables plants to adapt to continuous, often unfavorable and unpredictable changes in their environment. At the molecular level, adaptive advantages for plants are primarily provided by epigenetic machinery including DNA methylation, histone modifications, a...

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Autores principales: Lochmanová, Gabriela, Ihnatová, Ivana, Kuchaříková, Hana, Brabencová, Sylva, Zachová, Dagmar, Fajkus, Jiří, Zdráhal, Zbyněk, Fojtová, Miloslava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829310/
https://www.ncbi.nlm.nih.gov/pubmed/31615119
http://dx.doi.org/10.3390/ijms20205093
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author Lochmanová, Gabriela
Ihnatová, Ivana
Kuchaříková, Hana
Brabencová, Sylva
Zachová, Dagmar
Fajkus, Jiří
Zdráhal, Zbyněk
Fojtová, Miloslava
author_facet Lochmanová, Gabriela
Ihnatová, Ivana
Kuchaříková, Hana
Brabencová, Sylva
Zachová, Dagmar
Fajkus, Jiří
Zdráhal, Zbyněk
Fojtová, Miloslava
author_sort Lochmanová, Gabriela
collection PubMed
description A high degree of developmental plasticity enables plants to adapt to continuous, often unfavorable and unpredictable changes in their environment. At the molecular level, adaptive advantages for plants are primarily provided by epigenetic machinery including DNA methylation, histone modifications, and the activity of noncoding RNA molecules. Using a mass spectrometry-based proteomic approach, we examined the levels of acetylated histone peptide forms in Arabidopsis plants with a loss of function of histone deacetylase 6 (HDA6), and in plants germinated in the presence of HDA inhibitors trichostatin A (TSA) and sodium butyrate (NaB). Our analyses revealed particular lysine sites at histone sequences targeted by the HDA6 enzyme, and by TSA- and NaB-sensitive HDAs. Compared with plants exposed to drugs, more dramatic changes in the overall profiles of histone post-translational modifications were identified in hda6 mutants. However, loss of HDA6 was not sufficient by itself to induce hyperacetylation to the maximum degree, implying complementary activities of other HDAs. In contrast to hda6 mutants that did not exhibit any obvious phenotypic defects, the phenotypes of seedlings exposed to HDA inhibitors were markedly affected, showing that the effect of these drugs on early plant development is not limited to the modulation of histone acetylation levels.
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spelling pubmed-68293102019-11-18 Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications Lochmanová, Gabriela Ihnatová, Ivana Kuchaříková, Hana Brabencová, Sylva Zachová, Dagmar Fajkus, Jiří Zdráhal, Zbyněk Fojtová, Miloslava Int J Mol Sci Article A high degree of developmental plasticity enables plants to adapt to continuous, often unfavorable and unpredictable changes in their environment. At the molecular level, adaptive advantages for plants are primarily provided by epigenetic machinery including DNA methylation, histone modifications, and the activity of noncoding RNA molecules. Using a mass spectrometry-based proteomic approach, we examined the levels of acetylated histone peptide forms in Arabidopsis plants with a loss of function of histone deacetylase 6 (HDA6), and in plants germinated in the presence of HDA inhibitors trichostatin A (TSA) and sodium butyrate (NaB). Our analyses revealed particular lysine sites at histone sequences targeted by the HDA6 enzyme, and by TSA- and NaB-sensitive HDAs. Compared with plants exposed to drugs, more dramatic changes in the overall profiles of histone post-translational modifications were identified in hda6 mutants. However, loss of HDA6 was not sufficient by itself to induce hyperacetylation to the maximum degree, implying complementary activities of other HDAs. In contrast to hda6 mutants that did not exhibit any obvious phenotypic defects, the phenotypes of seedlings exposed to HDA inhibitors were markedly affected, showing that the effect of these drugs on early plant development is not limited to the modulation of histone acetylation levels. MDPI 2019-10-14 /pmc/articles/PMC6829310/ /pubmed/31615119 http://dx.doi.org/10.3390/ijms20205093 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lochmanová, Gabriela
Ihnatová, Ivana
Kuchaříková, Hana
Brabencová, Sylva
Zachová, Dagmar
Fajkus, Jiří
Zdráhal, Zbyněk
Fojtová, Miloslava
Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
title Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
title_full Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
title_fullStr Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
title_full_unstemmed Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
title_short Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
title_sort different modes of action of genetic and chemical downregulation of histone deacetylases with respect to plant development and histone modifications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829310/
https://www.ncbi.nlm.nih.gov/pubmed/31615119
http://dx.doi.org/10.3390/ijms20205093
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