Cargando…
Complementary Immunometabolic Effects of Exercise and PPARβ/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice
Regular aerobic exercise, independently of weight loss, improves metabolic and anti-inflammatory states, and can be regarded as beneficial in counteracting obesity-induced low-grade inflammation. However, it is still unknown how exercise alters immunometabolism in a context of dietary changes. Agoni...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829333/ https://www.ncbi.nlm.nih.gov/pubmed/31635041 http://dx.doi.org/10.3390/ijms20205182 |
_version_ | 1783465530389168128 |
---|---|
author | Le Garf, Sébastien Murdaca, Joseph Mothe-Satney, Isabelle Sibille, Brigitte Le Menn, Gwenaëlle Chinetti, Giulia Neels, Jaap G. Rousseau, Anne-Sophie |
author_facet | Le Garf, Sébastien Murdaca, Joseph Mothe-Satney, Isabelle Sibille, Brigitte Le Menn, Gwenaëlle Chinetti, Giulia Neels, Jaap G. Rousseau, Anne-Sophie |
author_sort | Le Garf, Sébastien |
collection | PubMed |
description | Regular aerobic exercise, independently of weight loss, improves metabolic and anti-inflammatory states, and can be regarded as beneficial in counteracting obesity-induced low-grade inflammation. However, it is still unknown how exercise alters immunometabolism in a context of dietary changes. Agonists of the Peroxisome Proliferator Activated-Receptor beta/delta (PPARβ/δ) have been studied this last decade as “exercise-mimetics”, which are potential therapies for metabolic diseases. In this study, we address the question of whether PPARβ/δ agonist treatment would improve the immunometabolic changes induced by exercise in diet-induced obese female mice, having switched from a high fat diet to a normal diet. 24 mice were assigned to groups according to an 8-week exercise training program and/or an 8-week treatment with 3 mg/kg/day of GW0742, a PPARβ/δ agonist. Our results show metabolic changes of peripheral lymphoid tissues with PPARβ/δ agonist (increase in fatty acid oxidation gene expression) or exercise (increase in AMPK activity) and a potentiating effect of the combination of both on the percentage of anti-inflammatory Foxp3+ T cells. Those effects are associated with a decreased visceral adipose tissue mass and skeletal muscle inflammation (TNF-α, Il-6, Il-1β mRNA level), an increase in skeletal muscle oxidative capacities (citrate synthase activity, endurance capacity), and insulin sensitivity. We conclude that a therapeutic approach targeting the PPARβ/δ pathway would improve obesity treatment. |
format | Online Article Text |
id | pubmed-6829333 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68293332019-11-18 Complementary Immunometabolic Effects of Exercise and PPARβ/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice Le Garf, Sébastien Murdaca, Joseph Mothe-Satney, Isabelle Sibille, Brigitte Le Menn, Gwenaëlle Chinetti, Giulia Neels, Jaap G. Rousseau, Anne-Sophie Int J Mol Sci Article Regular aerobic exercise, independently of weight loss, improves metabolic and anti-inflammatory states, and can be regarded as beneficial in counteracting obesity-induced low-grade inflammation. However, it is still unknown how exercise alters immunometabolism in a context of dietary changes. Agonists of the Peroxisome Proliferator Activated-Receptor beta/delta (PPARβ/δ) have been studied this last decade as “exercise-mimetics”, which are potential therapies for metabolic diseases. In this study, we address the question of whether PPARβ/δ agonist treatment would improve the immunometabolic changes induced by exercise in diet-induced obese female mice, having switched from a high fat diet to a normal diet. 24 mice were assigned to groups according to an 8-week exercise training program and/or an 8-week treatment with 3 mg/kg/day of GW0742, a PPARβ/δ agonist. Our results show metabolic changes of peripheral lymphoid tissues with PPARβ/δ agonist (increase in fatty acid oxidation gene expression) or exercise (increase in AMPK activity) and a potentiating effect of the combination of both on the percentage of anti-inflammatory Foxp3+ T cells. Those effects are associated with a decreased visceral adipose tissue mass and skeletal muscle inflammation (TNF-α, Il-6, Il-1β mRNA level), an increase in skeletal muscle oxidative capacities (citrate synthase activity, endurance capacity), and insulin sensitivity. We conclude that a therapeutic approach targeting the PPARβ/δ pathway would improve obesity treatment. MDPI 2019-10-19 /pmc/articles/PMC6829333/ /pubmed/31635041 http://dx.doi.org/10.3390/ijms20205182 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Le Garf, Sébastien Murdaca, Joseph Mothe-Satney, Isabelle Sibille, Brigitte Le Menn, Gwenaëlle Chinetti, Giulia Neels, Jaap G. Rousseau, Anne-Sophie Complementary Immunometabolic Effects of Exercise and PPARβ/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice |
title | Complementary Immunometabolic Effects of Exercise and PPARβ/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice |
title_full | Complementary Immunometabolic Effects of Exercise and PPARβ/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice |
title_fullStr | Complementary Immunometabolic Effects of Exercise and PPARβ/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice |
title_full_unstemmed | Complementary Immunometabolic Effects of Exercise and PPARβ/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice |
title_short | Complementary Immunometabolic Effects of Exercise and PPARβ/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice |
title_sort | complementary immunometabolic effects of exercise and pparβ/δ agonist in the context of diet-induced weight loss in obese female mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829333/ https://www.ncbi.nlm.nih.gov/pubmed/31635041 http://dx.doi.org/10.3390/ijms20205182 |
work_keys_str_mv | AT legarfsebastien complementaryimmunometaboliceffectsofexerciseandpparbdagonistinthecontextofdietinducedweightlossinobesefemalemice AT murdacajoseph complementaryimmunometaboliceffectsofexerciseandpparbdagonistinthecontextofdietinducedweightlossinobesefemalemice AT mothesatneyisabelle complementaryimmunometaboliceffectsofexerciseandpparbdagonistinthecontextofdietinducedweightlossinobesefemalemice AT sibillebrigitte complementaryimmunometaboliceffectsofexerciseandpparbdagonistinthecontextofdietinducedweightlossinobesefemalemice AT lemenngwenaelle complementaryimmunometaboliceffectsofexerciseandpparbdagonistinthecontextofdietinducedweightlossinobesefemalemice AT chinettigiulia complementaryimmunometaboliceffectsofexerciseandpparbdagonistinthecontextofdietinducedweightlossinobesefemalemice AT neelsjaapg complementaryimmunometaboliceffectsofexerciseandpparbdagonistinthecontextofdietinducedweightlossinobesefemalemice AT rousseauannesophie complementaryimmunometaboliceffectsofexerciseandpparbdagonistinthecontextofdietinducedweightlossinobesefemalemice |