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Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications

The use of ureteral stents to relieve urinary tract obstruction is still challenged by the problems of infection, encrustation, and compression, leading to the need for early removal procedures. Biodegradable ureteral stents, commonly made of polymers, have been proposed to overcome these problems....

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Detalles Bibliográficos
Autores principales: Paramitha, Devi, Chabaud, Stéphane, Bolduc, Stéphane, Hermawan, Hendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829415/
https://www.ncbi.nlm.nih.gov/pubmed/31614757
http://dx.doi.org/10.3390/ma12203325
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author Paramitha, Devi
Chabaud, Stéphane
Bolduc, Stéphane
Hermawan, Hendra
author_facet Paramitha, Devi
Chabaud, Stéphane
Bolduc, Stéphane
Hermawan, Hendra
author_sort Paramitha, Devi
collection PubMed
description The use of ureteral stents to relieve urinary tract obstruction is still challenged by the problems of infection, encrustation, and compression, leading to the need for early removal procedures. Biodegradable ureteral stents, commonly made of polymers, have been proposed to overcome these problems. Recently, absorbable metals have been considered as potential materials offering both biodegradation and strength. This work proposed zinc-based absorbable metals by firstly evaluating their cytocompatibility toward normal primary human urothelial cells using 2D and 3D assays. In the 2D assay, the cells were exposed to different concentrations of metal extracts (i.e., 10 mg/mL of Zn–1Mg and 8.75 mg/mL of Zn–0.5Al) for up to 3 days and found that their cytoskeletal networks were affected but were recovered at day 3, as observed by immunofluorescence. In the 3D ureteral wall tissue construct, the cells formed a multilayered urothelium, as found in native tissue, with the presence of tight junctions at the superficial layer and laminin at the basal layer, indicating a healthy tissue condition even with the presence of the metal samples for up to 7 days of exposure. The basal cells attached to the metal surface as seen in a natural spreading state with pseudopodia and fusiform morphologies, indicating that the metals were non-toxic.
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spelling pubmed-68294152019-11-18 Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications Paramitha, Devi Chabaud, Stéphane Bolduc, Stéphane Hermawan, Hendra Materials (Basel) Article The use of ureteral stents to relieve urinary tract obstruction is still challenged by the problems of infection, encrustation, and compression, leading to the need for early removal procedures. Biodegradable ureteral stents, commonly made of polymers, have been proposed to overcome these problems. Recently, absorbable metals have been considered as potential materials offering both biodegradation and strength. This work proposed zinc-based absorbable metals by firstly evaluating their cytocompatibility toward normal primary human urothelial cells using 2D and 3D assays. In the 2D assay, the cells were exposed to different concentrations of metal extracts (i.e., 10 mg/mL of Zn–1Mg and 8.75 mg/mL of Zn–0.5Al) for up to 3 days and found that their cytoskeletal networks were affected but were recovered at day 3, as observed by immunofluorescence. In the 3D ureteral wall tissue construct, the cells formed a multilayered urothelium, as found in native tissue, with the presence of tight junctions at the superficial layer and laminin at the basal layer, indicating a healthy tissue condition even with the presence of the metal samples for up to 7 days of exposure. The basal cells attached to the metal surface as seen in a natural spreading state with pseudopodia and fusiform morphologies, indicating that the metals were non-toxic. MDPI 2019-10-12 /pmc/articles/PMC6829415/ /pubmed/31614757 http://dx.doi.org/10.3390/ma12203325 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paramitha, Devi
Chabaud, Stéphane
Bolduc, Stéphane
Hermawan, Hendra
Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications
title Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications
title_full Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications
title_fullStr Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications
title_full_unstemmed Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications
title_short Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications
title_sort biological assessment of zn–based absorbable metals for ureteral stent applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829415/
https://www.ncbi.nlm.nih.gov/pubmed/31614757
http://dx.doi.org/10.3390/ma12203325
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