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Evolution of the Proto Sex-Chromosome in Solea senegalensis
Solea senegalensis is a flatfish belonging to the Soleidae family within the Pleuronectiformes order. It has a karyotype of 2n = 42 (FN = 60; 6M + 4 SM + 8 St + 24 T) and a XX/XY system. The first pair of metacentric chromosomes has been proposed as a proto sex-chromosome originated by a Robertsonia...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829477/ https://www.ncbi.nlm.nih.gov/pubmed/31618912 http://dx.doi.org/10.3390/ijms20205111 |
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author | Rodríguez, María Esther Molina, Belén Merlo, Manuel Alejandro Arias-Pérez, Alberto Portela-Bens, Silvia García-Angulo, Aglaya Cross, Ismael Liehr, Thomas Rebordinos, Laureana |
author_facet | Rodríguez, María Esther Molina, Belén Merlo, Manuel Alejandro Arias-Pérez, Alberto Portela-Bens, Silvia García-Angulo, Aglaya Cross, Ismael Liehr, Thomas Rebordinos, Laureana |
author_sort | Rodríguez, María Esther |
collection | PubMed |
description | Solea senegalensis is a flatfish belonging to the Soleidae family within the Pleuronectiformes order. It has a karyotype of 2n = 42 (FN = 60; 6M + 4 SM + 8 St + 24 T) and a XX/XY system. The first pair of metacentric chromosomes has been proposed as a proto sex-chromosome originated by a Robertsonian fusion between acrocentric chromosomes. In order to elucidate a possible evolutionary origin of this chromosome 1, studies of genomic synteny were carried out with eight fish species. A total of 88 genes annotated within of 14 BACs located in the chromosome 1 of S. senegalensis were used to elaborate syntenic maps. Six BACs (BAC5K5, BAC52C17, BAC53B20, BAC84K7, BAC56H24, and BAC48P7) were distributed in, at least, 5 chromosomes in the species studied, and a group of four genes from BAC53B20 (grsf1, rufy3, slc4a4 and npffr2) and genes from BAC48K7 (dmrt2, dmrt3, dmrt1, c9orf117, kank1 and fbp1) formed a conserved cluster in all species. The analysis of repetitive sequences showed that the number of retroelements and simple repeat per BAC showed its highest value in the subcentromeric region where 53B20, 16E16 and 48K7 BACs were localized. This region contains all the dmrt genes, which are associated with sex determination in some species. In addition, the presence of a satellite “chromosome Y” (motif length: 860 bp) was detected in this region. These findings allowed to trace an evolutionary trend for the large metacentric chromosome of S. senegalensis, throughout different rearrangements, which could be at an initial phase of differentiation as sex chromosome. |
format | Online Article Text |
id | pubmed-6829477 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68294772019-11-18 Evolution of the Proto Sex-Chromosome in Solea senegalensis Rodríguez, María Esther Molina, Belén Merlo, Manuel Alejandro Arias-Pérez, Alberto Portela-Bens, Silvia García-Angulo, Aglaya Cross, Ismael Liehr, Thomas Rebordinos, Laureana Int J Mol Sci Article Solea senegalensis is a flatfish belonging to the Soleidae family within the Pleuronectiformes order. It has a karyotype of 2n = 42 (FN = 60; 6M + 4 SM + 8 St + 24 T) and a XX/XY system. The first pair of metacentric chromosomes has been proposed as a proto sex-chromosome originated by a Robertsonian fusion between acrocentric chromosomes. In order to elucidate a possible evolutionary origin of this chromosome 1, studies of genomic synteny were carried out with eight fish species. A total of 88 genes annotated within of 14 BACs located in the chromosome 1 of S. senegalensis were used to elaborate syntenic maps. Six BACs (BAC5K5, BAC52C17, BAC53B20, BAC84K7, BAC56H24, and BAC48P7) were distributed in, at least, 5 chromosomes in the species studied, and a group of four genes from BAC53B20 (grsf1, rufy3, slc4a4 and npffr2) and genes from BAC48K7 (dmrt2, dmrt3, dmrt1, c9orf117, kank1 and fbp1) formed a conserved cluster in all species. The analysis of repetitive sequences showed that the number of retroelements and simple repeat per BAC showed its highest value in the subcentromeric region where 53B20, 16E16 and 48K7 BACs were localized. This region contains all the dmrt genes, which are associated with sex determination in some species. In addition, the presence of a satellite “chromosome Y” (motif length: 860 bp) was detected in this region. These findings allowed to trace an evolutionary trend for the large metacentric chromosome of S. senegalensis, throughout different rearrangements, which could be at an initial phase of differentiation as sex chromosome. MDPI 2019-10-15 /pmc/articles/PMC6829477/ /pubmed/31618912 http://dx.doi.org/10.3390/ijms20205111 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rodríguez, María Esther Molina, Belén Merlo, Manuel Alejandro Arias-Pérez, Alberto Portela-Bens, Silvia García-Angulo, Aglaya Cross, Ismael Liehr, Thomas Rebordinos, Laureana Evolution of the Proto Sex-Chromosome in Solea senegalensis |
title | Evolution of the Proto Sex-Chromosome in Solea senegalensis |
title_full | Evolution of the Proto Sex-Chromosome in Solea senegalensis |
title_fullStr | Evolution of the Proto Sex-Chromosome in Solea senegalensis |
title_full_unstemmed | Evolution of the Proto Sex-Chromosome in Solea senegalensis |
title_short | Evolution of the Proto Sex-Chromosome in Solea senegalensis |
title_sort | evolution of the proto sex-chromosome in solea senegalensis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829477/ https://www.ncbi.nlm.nih.gov/pubmed/31618912 http://dx.doi.org/10.3390/ijms20205111 |
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