Cargando…

Methyl Donor Deficiency during Gestation and Lactation in the Rat Affects the Expression of Neuropeptides and Related Receptors in the Hypothalamus

The micronutrients vitamins B9 and B12 act as methyl donors in the one-carbon metabolism involved in transmethylation reactions which critically influence epigenetic mechanisms and gene expression. Both vitamins are essential for proper development, and their deficiency during pregnancy has been ass...

Descripción completa

Detalles Bibliográficos
Autores principales: Saber Cherif, Lynda, Pourié, Grégory, Geoffroy, Andréa, Julien, Amélia, Helle, Déborah, Robert, Aurélie, Umoret, Rémy, Guéant, Jean-Louis, Bossenmeyer-Pourié, Carine, Daval, Jean-Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829491/
https://www.ncbi.nlm.nih.gov/pubmed/31615150
http://dx.doi.org/10.3390/ijms20205097
_version_ 1783465568936919040
author Saber Cherif, Lynda
Pourié, Grégory
Geoffroy, Andréa
Julien, Amélia
Helle, Déborah
Robert, Aurélie
Umoret, Rémy
Guéant, Jean-Louis
Bossenmeyer-Pourié, Carine
Daval, Jean-Luc
author_facet Saber Cherif, Lynda
Pourié, Grégory
Geoffroy, Andréa
Julien, Amélia
Helle, Déborah
Robert, Aurélie
Umoret, Rémy
Guéant, Jean-Louis
Bossenmeyer-Pourié, Carine
Daval, Jean-Luc
author_sort Saber Cherif, Lynda
collection PubMed
description The micronutrients vitamins B9 and B12 act as methyl donors in the one-carbon metabolism involved in transmethylation reactions which critically influence epigenetic mechanisms and gene expression. Both vitamins are essential for proper development, and their deficiency during pregnancy has been associated with a wide range of disorders, including persisting growth retardation. Energy homeostasis and feeding are centrally regulated by the hypothalamus which integrates peripheral signals and acts through several orexigenic and anorexigenic mediators. We studied this regulating system in a rat model of methyl donor deficiency during gestation and lactation. At weaning, a predominance of the anorexigenic pathway was observed in deficient pups, with increased plasma peptide YY and increased hypothalamic pro-opiomelanocortin (POMC) mRNA, in line with abnormal leptin, ghrelin, and insulin secretion and/or signaling during critical periods of fetal and/or postnatal development of the hypothalamus. These results suggest that early methyl donor deficiency can affect the development and function of energy balance circuits, resulting in growth and weight deficits. Maternal administration of folic acid (3 mg/kg/day) during the perinatal period tended to rectify peripheral metabolic signaling and central neuropeptide and receptor expression, leading to reduced growth retardation.
format Online
Article
Text
id pubmed-6829491
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68294912019-11-18 Methyl Donor Deficiency during Gestation and Lactation in the Rat Affects the Expression of Neuropeptides and Related Receptors in the Hypothalamus Saber Cherif, Lynda Pourié, Grégory Geoffroy, Andréa Julien, Amélia Helle, Déborah Robert, Aurélie Umoret, Rémy Guéant, Jean-Louis Bossenmeyer-Pourié, Carine Daval, Jean-Luc Int J Mol Sci Article The micronutrients vitamins B9 and B12 act as methyl donors in the one-carbon metabolism involved in transmethylation reactions which critically influence epigenetic mechanisms and gene expression. Both vitamins are essential for proper development, and their deficiency during pregnancy has been associated with a wide range of disorders, including persisting growth retardation. Energy homeostasis and feeding are centrally regulated by the hypothalamus which integrates peripheral signals and acts through several orexigenic and anorexigenic mediators. We studied this regulating system in a rat model of methyl donor deficiency during gestation and lactation. At weaning, a predominance of the anorexigenic pathway was observed in deficient pups, with increased plasma peptide YY and increased hypothalamic pro-opiomelanocortin (POMC) mRNA, in line with abnormal leptin, ghrelin, and insulin secretion and/or signaling during critical periods of fetal and/or postnatal development of the hypothalamus. These results suggest that early methyl donor deficiency can affect the development and function of energy balance circuits, resulting in growth and weight deficits. Maternal administration of folic acid (3 mg/kg/day) during the perinatal period tended to rectify peripheral metabolic signaling and central neuropeptide and receptor expression, leading to reduced growth retardation. MDPI 2019-10-14 /pmc/articles/PMC6829491/ /pubmed/31615150 http://dx.doi.org/10.3390/ijms20205097 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saber Cherif, Lynda
Pourié, Grégory
Geoffroy, Andréa
Julien, Amélia
Helle, Déborah
Robert, Aurélie
Umoret, Rémy
Guéant, Jean-Louis
Bossenmeyer-Pourié, Carine
Daval, Jean-Luc
Methyl Donor Deficiency during Gestation and Lactation in the Rat Affects the Expression of Neuropeptides and Related Receptors in the Hypothalamus
title Methyl Donor Deficiency during Gestation and Lactation in the Rat Affects the Expression of Neuropeptides and Related Receptors in the Hypothalamus
title_full Methyl Donor Deficiency during Gestation and Lactation in the Rat Affects the Expression of Neuropeptides and Related Receptors in the Hypothalamus
title_fullStr Methyl Donor Deficiency during Gestation and Lactation in the Rat Affects the Expression of Neuropeptides and Related Receptors in the Hypothalamus
title_full_unstemmed Methyl Donor Deficiency during Gestation and Lactation in the Rat Affects the Expression of Neuropeptides and Related Receptors in the Hypothalamus
title_short Methyl Donor Deficiency during Gestation and Lactation in the Rat Affects the Expression of Neuropeptides and Related Receptors in the Hypothalamus
title_sort methyl donor deficiency during gestation and lactation in the rat affects the expression of neuropeptides and related receptors in the hypothalamus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829491/
https://www.ncbi.nlm.nih.gov/pubmed/31615150
http://dx.doi.org/10.3390/ijms20205097
work_keys_str_mv AT sabercheriflynda methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT pouriegregory methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT geoffroyandrea methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT julienamelia methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT helledeborah methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT robertaurelie methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT umoretremy methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT gueantjeanlouis methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT bossenmeyerpouriecarine methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus
AT davaljeanluc methyldonordeficiencyduringgestationandlactationintherataffectstheexpressionofneuropeptidesandrelatedreceptorsinthehypothalamus