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Quantitative determination of cellular [Na(+)] by fluorescence lifetime imaging with CoroNaGreen

Fluorescence lifetime imaging microscopy (FLIM) with fluorescent ion sensors enables the measurement of ion concentrations based on the detection of photon emission events after brief excitation with a pulsed laser source. In contrast to intensity-based imaging, it is independent of dye concentratio...

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Detalles Bibliográficos
Autores principales: Meyer, Jan, Untiet, Verena, Fahlke, Christoph, Gensch, Thomas, Rose, Christine R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829561/
https://www.ncbi.nlm.nih.gov/pubmed/31597684
http://dx.doi.org/10.1085/jgp.201912404